Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat

Films formulated with gelatin and chitosan (GL/CH) or chitosan (CH), without or with 0.3% and 0.5% concentrations of rice bran extract (RBE), have been developed. The migrations of rice bran extract and the antioxidant and antimicrobial properties in vitro have been assessed. The effects of the film...

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Main Authors: María Cabeza de Vaca, Rosario Ramírez, Javier Rocha-Pimienta, David Tejerina, Jonathan Delgado-Adámez
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Gels
Subjects:
Online Access:https://www.mdpi.com/2310-2861/11/5/338
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author María Cabeza de Vaca
Rosario Ramírez
Javier Rocha-Pimienta
David Tejerina
Jonathan Delgado-Adámez
author_facet María Cabeza de Vaca
Rosario Ramírez
Javier Rocha-Pimienta
David Tejerina
Jonathan Delgado-Adámez
author_sort María Cabeza de Vaca
collection DOAJ
description Films formulated with gelatin and chitosan (GL/CH) or chitosan (CH), without or with 0.3% and 0.5% concentrations of rice bran extract (RBE), have been developed. The migrations of rice bran extract and the antioxidant and antimicrobial properties in vitro have been assessed. The effects of the film formulations in maintaining color stability, oxidative status and microbial loads on fresh pork meat during 9 days of refrigerated storage were studied. For the films, releases of γ-oryzanol only were observed in low polarity simulant. The highest migrations and antioxidant activity were related to gelatine films, enhanced with the addition of rice bran extract. Only chitosan films showed antimicrobial activity in vitro against <i>Escherichia coli</i> and <i>Listeria innocua</i>, reaching decreases of 7.68 and 8.06 Log CFU at 72 h, respectively. Both gelatin/chitosan and chitosan films prevented the color changes in meat during storage, preventing the paleness, and chitosan films also provoked an increment of redness until 2.88 units of CIE b* at day 9. The films did not prevent either lipid or protein oxidation in meat, despite the rice bran extract inclusion, even increasing the lipid oxidations with chitosan films. However, all films helped to control the microbial counts in meat throughout all the storage, with chitosan being the most effective films, especially with the addition of RBE. Overall, gelatin/chitosan and chitosan films offer a sustainable alternative for fresh pork meat packaging.
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spelling doaj-art-9fd3d64ebec64cc1a4da716dd956d50d2025-08-20T02:33:57ZengMDPI AGGels2310-28612025-04-0111533810.3390/gels11050338Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork MeatMaría Cabeza de Vaca0Rosario Ramírez1Javier Rocha-Pimienta2David Tejerina3Jonathan Delgado-Adámez4Technological Institute of Food and Agriculture (INTAEX), Center for Scientific and Technological Research of Extremadura (CICYTEX), Avda, Adolfo Suárez s/n, 06007 Badajoz, SpainTechnological Institute of Food and Agriculture (INTAEX), Center for Scientific and Technological Research of Extremadura (CICYTEX), Avda, Adolfo Suárez s/n, 06007 Badajoz, SpainTechnological Institute of Food and Agriculture (INTAEX), Center for Scientific and Technological Research of Extremadura (CICYTEX), Avda, Adolfo Suárez s/n, 06007 Badajoz, SpainTechnological Institute of Food and Agriculture (INTAEX), Center for Scientific and Technological Research of Extremadura (CICYTEX), Avda, Adolfo Suárez s/n, 06007 Badajoz, SpainTechnological Institute of Food and Agriculture (INTAEX), Center for Scientific and Technological Research of Extremadura (CICYTEX), Avda, Adolfo Suárez s/n, 06007 Badajoz, SpainFilms formulated with gelatin and chitosan (GL/CH) or chitosan (CH), without or with 0.3% and 0.5% concentrations of rice bran extract (RBE), have been developed. The migrations of rice bran extract and the antioxidant and antimicrobial properties in vitro have been assessed. The effects of the film formulations in maintaining color stability, oxidative status and microbial loads on fresh pork meat during 9 days of refrigerated storage were studied. For the films, releases of γ-oryzanol only were observed in low polarity simulant. The highest migrations and antioxidant activity were related to gelatine films, enhanced with the addition of rice bran extract. Only chitosan films showed antimicrobial activity in vitro against <i>Escherichia coli</i> and <i>Listeria innocua</i>, reaching decreases of 7.68 and 8.06 Log CFU at 72 h, respectively. Both gelatin/chitosan and chitosan films prevented the color changes in meat during storage, preventing the paleness, and chitosan films also provoked an increment of redness until 2.88 units of CIE b* at day 9. The films did not prevent either lipid or protein oxidation in meat, despite the rice bran extract inclusion, even increasing the lipid oxidations with chitosan films. However, all films helped to control the microbial counts in meat throughout all the storage, with chitosan being the most effective films, especially with the addition of RBE. Overall, gelatin/chitosan and chitosan films offer a sustainable alternative for fresh pork meat packaging.https://www.mdpi.com/2310-2861/11/5/338active filmgelatinchitosanactive biodegradable packagingmeat preservationrice bran extract (RBE)
spellingShingle María Cabeza de Vaca
Rosario Ramírez
Javier Rocha-Pimienta
David Tejerina
Jonathan Delgado-Adámez
Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
Gels
active film
gelatin
chitosan
active biodegradable packaging
meat preservation
rice bran extract (RBE)
title Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
title_full Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
title_fullStr Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
title_full_unstemmed Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
title_short Effects of Gelatin/Chitosan and Chitosan Active Films with Rice Bran Extract for the Preservation of Fresh Pork Meat
title_sort effects of gelatin chitosan and chitosan active films with rice bran extract for the preservation of fresh pork meat
topic active film
gelatin
chitosan
active biodegradable packaging
meat preservation
rice bran extract (RBE)
url https://www.mdpi.com/2310-2861/11/5/338
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