Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State
Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) are the pluripotent stem cells (PSCs), derived from the inner cell mass (ICM) of preimplantation embryos at embryonic day 3.5 (E3.5) and postimplantation embryos at E5.5-E7.5, respectively. Depending on their environment, PSC...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/8818356 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849400138993238016 |
|---|---|
| author | A. S. Devika Anna Montebaur S. Saravanan Raghu Bhushan Frederic Koch Smita Sudheer |
| author_facet | A. S. Devika Anna Montebaur S. Saravanan Raghu Bhushan Frederic Koch Smita Sudheer |
| author_sort | A. S. Devika |
| collection | DOAJ |
| description | Mouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) are the pluripotent stem cells (PSCs), derived from the inner cell mass (ICM) of preimplantation embryos at embryonic day 3.5 (E3.5) and postimplantation embryos at E5.5-E7.5, respectively. Depending on their environment, PSCs can exist in the so-called naïve (ESCs) or primed (EpiSCs) states. Exposure to EpiSC or human ESC (hESC) culture condition can convert mESCs towards an EpiSC-like state. Here, we show that the undifferentiated epiblast state is however not stabilized in a sustained manner when exposing mESCs to hESC or EpiSC culture condition. Rather, prolonged exposure to EpiSC condition promotes a transition to a primitive streak- (PS-) like state via an unbiased epiblast-like intermediate. We show that the Brachyury-positive PS-like state is likely promoted by endogenous WNT signaling, highlighting a possible species difference between mouse epiblast-like stem cells and human Embryonic Stem Cells. |
| format | Article |
| id | doaj-art-9fcc7a9bf47c41d8a0daa6ee7b97c6da |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9fcc7a9bf47c41d8a0daa6ee7b97c6da2025-08-20T03:38:09ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/88183568818356Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast StateA. S. Devika0Anna Montebaur1S. Saravanan2Raghu Bhushan3Frederic Koch4Smita Sudheer5Stem Cell Laboratory, Department of Genomic Science, Krishna Building, Central University of Kerala, Tejaswini Hills, Periye. P. O, Kasaragod District, Kerala 671316, IndiaDepartment of Developmental Genetics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, GermanyStem Cell Laboratory, Department of Genomic Science, Krishna Building, Central University of Kerala, Tejaswini Hills, Periye. P. O, Kasaragod District, Kerala 671316, IndiaYenepoya (Deemed to be University), University Road, Deralakatte, Mangalore, 575018 Karnataka, IndiaDepartment of Developmental Genetics, Max Planck Institute for Molecular Genetics, Ihnestrasse 63-73, 14195 Berlin, GermanyStem Cell Laboratory, Department of Genomic Science, Krishna Building, Central University of Kerala, Tejaswini Hills, Periye. P. O, Kasaragod District, Kerala 671316, IndiaMouse embryonic stem cells (mESCs) and mouse epiblast stem cells (mEpiSCs) are the pluripotent stem cells (PSCs), derived from the inner cell mass (ICM) of preimplantation embryos at embryonic day 3.5 (E3.5) and postimplantation embryos at E5.5-E7.5, respectively. Depending on their environment, PSCs can exist in the so-called naïve (ESCs) or primed (EpiSCs) states. Exposure to EpiSC or human ESC (hESC) culture condition can convert mESCs towards an EpiSC-like state. Here, we show that the undifferentiated epiblast state is however not stabilized in a sustained manner when exposing mESCs to hESC or EpiSC culture condition. Rather, prolonged exposure to EpiSC condition promotes a transition to a primitive streak- (PS-) like state via an unbiased epiblast-like intermediate. We show that the Brachyury-positive PS-like state is likely promoted by endogenous WNT signaling, highlighting a possible species difference between mouse epiblast-like stem cells and human Embryonic Stem Cells.http://dx.doi.org/10.1155/2021/8818356 |
| spellingShingle | A. S. Devika Anna Montebaur S. Saravanan Raghu Bhushan Frederic Koch Smita Sudheer Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State Stem Cells International |
| title | Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State |
| title_full | Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State |
| title_fullStr | Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State |
| title_full_unstemmed | Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State |
| title_short | Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State |
| title_sort | human es cell culture conditions fail to preserve the mouse epiblast state |
| url | http://dx.doi.org/10.1155/2021/8818356 |
| work_keys_str_mv | AT asdevika humanescellcultureconditionsfailtopreservethemouseepiblaststate AT annamontebaur humanescellcultureconditionsfailtopreservethemouseepiblaststate AT ssaravanan humanescellcultureconditionsfailtopreservethemouseepiblaststate AT raghubhushan humanescellcultureconditionsfailtopreservethemouseepiblaststate AT frederickoch humanescellcultureconditionsfailtopreservethemouseepiblaststate AT smitasudheer humanescellcultureconditionsfailtopreservethemouseepiblaststate |