Genome <i>De Novo</i> (WGS) Sequence Resource of the <i>Lasiodiplodia theobromae</i> Bot-2018-LT45 Isolate Causing Dieback in Apple

Genome <i>De Novo</i> (WGS) Sequence Resource of the <i>Lasiodiplodia theobromae</i> Bot-2018-LT45 Isolate Causing Dieback in Apple

<i>Lasiodiplodia theobromae</i> is a pathogenic fungus associated with tropical perennial fruit plants worldwide. In apple trees, <i>L. theobromae</i> causes dieback and canker, a disease that affects the architecture of the wood producing the progressive death of branches an...

Full description

Saved in:
Bibliographic Details
Main Authors: Adrián V. Valdez-Tenezaca, Sergio A. Hernández Covarrubias, Alexis G. Murillo Carrasco, Matías I. Guerra Peñalosa, Jean F. Castro Figueroa, M. Ernesto Delgado Fernández, José A. Corona-Gómez, Gonzalo A. Díaz Ulloa
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:International Journal of Plant Biology
Subjects:
apple
canker and dieback
genome
<i>Lasiodiplodia theobromae</i>
whole genome sequencing
Online Access:https://www.mdpi.com/2037-0164/16/1/10
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:<i>Lasiodiplodia theobromae</i> is a pathogenic fungus associated with tropical perennial fruit plants worldwide. In apple trees, <i>L. theobromae</i> causes dieback and canker, a disease that affects the architecture of the wood producing the progressive death of branches and stems, from the tips to the base, invading the vascular tissue, manifesting necrotic lesions in the bark, impeding the flow of nutrients and water. The present work reports the whole genome <i>de novo</i> sequencing (WGS) of <i>L. theobromae</i> strain Bot-2018-LT45 isolated from apple trees with dieback symptoms. Genomic DNA of <i>L. theobromae</i> was sequenced using Illumina paired-end short-read technology (NovaSeq6000) and PacBio SMRTbell<sup>TM</sup> (Single Molecule, Real-Time) long-read technology. The genome size was 44.17 Mb. Then, assembly and annotation revealed a total of 12,948 genes of which 11,634 encoded proteins. The genome was assembled into 34 contigs with an N50 (Mb) value of 3.23. This study is the first report of the <i>L. theobromae</i> genome <i>de novo</i> obtained from apple trees with dieback and canker symptoms in the Maule Region, Chile. This genetic information may set the basis for future study of the mechanisms of <i>L. theobromae</i> and establish the possibility of specific molecular improvements for the control of dieback and canker.
ISSN:2037-0164

Similar Items