Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash.
The morbidity and mortality related to respiratory tract diseases is enormous, with hundreds of millions of individuals afflicted and four million people dying each year. Understanding the immunological processes in the mucosa that govern outcome following pathogenic encounter could lead to novel th...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169805&type=printable |
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| author | Simon P Jochems Katherine Piddock Jamie Rylance Hugh Adler Beatriz F Carniel Andrea Collins Jenna F Gritzfeld Carole Hancock Helen Hill Jesus Reiné Alexandra Seddon Carla Solórzano Syba Sunny Ashleigh Trimble Angela D Wright Seher Zaidi Stephen B Gordon Daniela M Ferreira |
| author_facet | Simon P Jochems Katherine Piddock Jamie Rylance Hugh Adler Beatriz F Carniel Andrea Collins Jenna F Gritzfeld Carole Hancock Helen Hill Jesus Reiné Alexandra Seddon Carla Solórzano Syba Sunny Ashleigh Trimble Angela D Wright Seher Zaidi Stephen B Gordon Daniela M Ferreira |
| author_sort | Simon P Jochems |
| collection | DOAJ |
| description | The morbidity and mortality related to respiratory tract diseases is enormous, with hundreds of millions of individuals afflicted and four million people dying each year. Understanding the immunological processes in the mucosa that govern outcome following pathogenic encounter could lead to novel therapies. There is a need to study responses at mucosal surfaces in humans for two reasons: (i) Immunological findings in mice, or other animals, often fail to translate to humans. (ii) Compartmentalization of the immune system dictates a need to study sites where pathogens reside. In this manuscript, we describe two novel non-invasive nasal mucosal microsampling techniques and their use for measuring immunological parameters: 1) using nasal curettes to collect cells from the inferior turbinate and; 2) absorptive matrices to collect nasal lining fluid. Both techniques were well tolerated and yielded reproducible and robust data. We demonstrated differences in immune populations and activation state in nasal mucosa compared to blood as well as compared to nasopharyngeal lumen in healthy adults. We also found superior cytokine detection with absorptive matrices compared to nasal wash. These techniques are promising new tools that will facilitate studies of the immunological signatures underlying susceptibility and resistance to respiratory infections. |
| format | Article |
| id | doaj-art-9fc1a4b9902f4cd0b85040843a1bbcfa |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-9fc1a4b9902f4cd0b85040843a1bbcfa2025-08-20T03:24:29ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01121e016980510.1371/journal.pone.0169805Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash.Simon P JochemsKatherine PiddockJamie RylanceHugh AdlerBeatriz F CarnielAndrea CollinsJenna F GritzfeldCarole HancockHelen HillJesus ReinéAlexandra SeddonCarla SolórzanoSyba SunnyAshleigh TrimbleAngela D WrightSeher ZaidiStephen B GordonDaniela M FerreiraThe morbidity and mortality related to respiratory tract diseases is enormous, with hundreds of millions of individuals afflicted and four million people dying each year. Understanding the immunological processes in the mucosa that govern outcome following pathogenic encounter could lead to novel therapies. There is a need to study responses at mucosal surfaces in humans for two reasons: (i) Immunological findings in mice, or other animals, often fail to translate to humans. (ii) Compartmentalization of the immune system dictates a need to study sites where pathogens reside. In this manuscript, we describe two novel non-invasive nasal mucosal microsampling techniques and their use for measuring immunological parameters: 1) using nasal curettes to collect cells from the inferior turbinate and; 2) absorptive matrices to collect nasal lining fluid. Both techniques were well tolerated and yielded reproducible and robust data. We demonstrated differences in immune populations and activation state in nasal mucosa compared to blood as well as compared to nasopharyngeal lumen in healthy adults. We also found superior cytokine detection with absorptive matrices compared to nasal wash. These techniques are promising new tools that will facilitate studies of the immunological signatures underlying susceptibility and resistance to respiratory infections.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169805&type=printable |
| spellingShingle | Simon P Jochems Katherine Piddock Jamie Rylance Hugh Adler Beatriz F Carniel Andrea Collins Jenna F Gritzfeld Carole Hancock Helen Hill Jesus Reiné Alexandra Seddon Carla Solórzano Syba Sunny Ashleigh Trimble Angela D Wright Seher Zaidi Stephen B Gordon Daniela M Ferreira Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. PLoS ONE |
| title | Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. |
| title_full | Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. |
| title_fullStr | Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. |
| title_full_unstemmed | Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. |
| title_short | Novel Analysis of Immune Cells from Nasal Microbiopsy Demonstrates Reliable, Reproducible Data for Immune Populations, and Superior Cytokine Detection Compared to Nasal Wash. |
| title_sort | novel analysis of immune cells from nasal microbiopsy demonstrates reliable reproducible data for immune populations and superior cytokine detection compared to nasal wash |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0169805&type=printable |
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