Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients
Objective: To evaluate and compare the associations of VEGFAserum levels and SNPs (rs1570360, rs699947, rs3025033, and rs2146323) with periodontitis in study participants grouped by gender. Methods: The study enrolled 261 patients with periodontitis and 441 healthy controls as a reference group....
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Medical Journals Sweden
2024-09-01
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| Series: | Acta Odontologica Scandinavica |
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| Online Access: | https://medicaljournalssweden.se/actaodontologica/article/view/42042 |
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| author | Albertas Kriauciunas Gediminas Zekonis Greta Gedvilaite Monika Duseikaitė Enrika Pileckaitė Ingrida Pacauskiene Rasa Liutkeviciene |
| author_facet | Albertas Kriauciunas Gediminas Zekonis Greta Gedvilaite Monika Duseikaitė Enrika Pileckaitė Ingrida Pacauskiene Rasa Liutkeviciene |
| author_sort | Albertas Kriauciunas |
| collection | DOAJ |
| description |
Objective: To evaluate and compare the associations of VEGFAserum levels and SNPs (rs1570360, rs699947, rs3025033, and rs2146323) with periodontitis in study participants grouped by gender.
Methods: The study enrolled 261 patients with periodontitis and 441 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNAsalting-out method was used for DNAextraction from peripheral venous blood. Genotyping of VEGFA(rs1570360, rs699947, rs3025033, and rs2146323) was performed using real-time polymerase chain reaction (RT-PCR) and serum level analysis was done for 80 individuals − 40 periodontitis-affected patients and 40 reference group subjects.
Results: The analysis of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) showed that the rs3025033 GG genotype was less frequent in the periodontitis group than in the reference group (1.6% vs. 5.7%,p = 0.008). VEGFAserum levels were not statistically significantly different between periodontitis patients and reference group subjects (554.29 (522.38) ng/ml vs. 581.32 (348.16) ng/ ml, p = 0.786). Individuals carrying rs1570360, rs699947, rs3025033, and rs2146323 haplotype A-A-G-Ahad decreased risks of periodontitis, while rare haplotype of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) was associated with increased odds of periodontitis (OR= 0.42; 95% CI: 0.20–0.85; p < 0.017; OR= 4.08; 95% CI: 1.86–8.94; p < 0.0001, respectively).
Conclusion: The rs3025033 GG genotype and the rs1570360, rs699947, rs3025033, and rs2146323 A-A-G-Ahaplotypes may play a protective role in the development of periodontitis, but a less common haplotype of the same VEGFApolymorphism may be associated with the risk of developing periodontitis.
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| format | Article |
| id | doaj-art-9fc04ff7e1f34d04b114fe224b40cb94 |
| institution | OA Journals |
| issn | 0001-6357 1502-3850 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Medical Journals Sweden |
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| series | Acta Odontologica Scandinavica |
| spelling | doaj-art-9fc04ff7e1f34d04b114fe224b40cb942025-08-20T01:55:57ZengMedical Journals SwedenActa Odontologica Scandinavica0001-63571502-38502024-09-0182110.1080/00016357.2023.2260000Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patientsAlbertas Kriauciunas0Gediminas Zekonis1Greta Gedvilaite2Monika Duseikaitė3Enrika Pileckaitė4Ingrida Pacauskiene5Rasa Liutkeviciene6Department of Prosthodontics, Lithuanian University of Health Sciences, Kaunas, LithuaniaDepartment of Prosthodontics, Lithuanian University of Health Sciences, Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania; Medical faculty, Lithuanian University of Health Sciences, Kaunas, LithuaniaMedical faculty, Lithuanian University of Health Sciences, Kaunas, Lithuania; Pharmacy faculty, Lithuanian University of Health Sciences, Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania; Medical faculty, Lithuanian University of Health Sciences, Kaunas, LithuaniaDepartment of Dental and Oral Pathology, Lithuanian University of Health Sciences, Kaunas, LithuaniaLaboratory of Ophthalmology, Institute of Neuroscience, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania Objective: To evaluate and compare the associations of VEGFAserum levels and SNPs (rs1570360, rs699947, rs3025033, and rs2146323) with periodontitis in study participants grouped by gender. Methods: The study enrolled 261 patients with periodontitis and 441 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNAsalting-out method was used for DNAextraction from peripheral venous blood. Genotyping of VEGFA(rs1570360, rs699947, rs3025033, and rs2146323) was performed using real-time polymerase chain reaction (RT-PCR) and serum level analysis was done for 80 individuals − 40 periodontitis-affected patients and 40 reference group subjects. Results: The analysis of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) showed that the rs3025033 GG genotype was less frequent in the periodontitis group than in the reference group (1.6% vs. 5.7%,p = 0.008). VEGFAserum levels were not statistically significantly different between periodontitis patients and reference group subjects (554.29 (522.38) ng/ml vs. 581.32 (348.16) ng/ ml, p = 0.786). Individuals carrying rs1570360, rs699947, rs3025033, and rs2146323 haplotype A-A-G-Ahad decreased risks of periodontitis, while rare haplotype of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) was associated with increased odds of periodontitis (OR= 0.42; 95% CI: 0.20–0.85; p < 0.017; OR= 4.08; 95% CI: 1.86–8.94; p < 0.0001, respectively). Conclusion: The rs3025033 GG genotype and the rs1570360, rs699947, rs3025033, and rs2146323 A-A-G-Ahaplotypes may play a protective role in the development of periodontitis, but a less common haplotype of the same VEGFApolymorphism may be associated with the risk of developing periodontitis. https://medicaljournalssweden.se/actaodontologica/article/view/42042PeriodontitisVEGFArs1570360rs699947rs3025033rs2146323) |
| spellingShingle | Albertas Kriauciunas Gediminas Zekonis Greta Gedvilaite Monika Duseikaitė Enrika Pileckaitė Ingrida Pacauskiene Rasa Liutkeviciene Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients Acta Odontologica Scandinavica Periodontitis VEGFA rs1570360 rs699947 rs3025033 rs2146323) |
| title | Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| title_full | Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| title_fullStr | Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| title_full_unstemmed | Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| title_short | Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| title_sort | vascular endothelial growth factor a serum levels and common gene polymorphisms in generalized periodontitis affected patients |
| topic | Periodontitis VEGFA rs1570360 rs699947 rs3025033 rs2146323) |
| url | https://medicaljournalssweden.se/actaodontologica/article/view/42042 |
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