Vascular Endothelial Growth Factor A serum levels and common gene polymorphisms in generalized periodontitis affected patients
Objective: To evaluate and compare the associations of VEGFAserum levels and SNPs (rs1570360, rs699947, rs3025033, and rs2146323) with periodontitis in study participants grouped by gender. Methods: The study enrolled 261 patients with periodontitis and 441 healthy controls as a reference group....
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Medical Journals Sweden
2024-09-01
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| Series: | Acta Odontologica Scandinavica |
| Subjects: | |
| Online Access: | https://medicaljournalssweden.se/actaodontologica/article/view/42042 |
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| Summary: | Objective: To evaluate and compare the associations of VEGFAserum levels and SNPs (rs1570360, rs699947, rs3025033, and rs2146323) with periodontitis in study participants grouped by gender.
Methods: The study enrolled 261 patients with periodontitis and 441 healthy controls as a reference group. Patients underwent periodontal examination and radiographic analysis to confirm the periodontitis diagnosis. Blood samples were collected, and the DNAsalting-out method was used for DNAextraction from peripheral venous blood. Genotyping of VEGFA(rs1570360, rs699947, rs3025033, and rs2146323) was performed using real-time polymerase chain reaction (RT-PCR) and serum level analysis was done for 80 individuals − 40 periodontitis-affected patients and 40 reference group subjects.
Results: The analysis of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) showed that the rs3025033 GG genotype was less frequent in the periodontitis group than in the reference group (1.6% vs. 5.7%,p = 0.008). VEGFAserum levels were not statistically significantly different between periodontitis patients and reference group subjects (554.29 (522.38) ng/ml vs. 581.32 (348.16) ng/ ml, p = 0.786). Individuals carrying rs1570360, rs699947, rs3025033, and rs2146323 haplotype A-A-G-Ahad decreased risks of periodontitis, while rare haplotype of VEGFA (rs1570360, rs699947, rs3025033, and rs2146323) was associated with increased odds of periodontitis (OR= 0.42; 95% CI: 0.20–0.85; p < 0.017; OR= 4.08; 95% CI: 1.86–8.94; p < 0.0001, respectively).
Conclusion: The rs3025033 GG genotype and the rs1570360, rs699947, rs3025033, and rs2146323 A-A-G-Ahaplotypes may play a protective role in the development of periodontitis, but a less common haplotype of the same VEGFApolymorphism may be associated with the risk of developing periodontitis.
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| ISSN: | 0001-6357 1502-3850 |