17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia
Summary: Mice administered an inhibin antiserum, equine chorionic gonadotropin (eCG) mixture called CARD HyperOva (OVA)/human chorionic gonadotropin (hCG), ovulate more oocytes than those administered eCG/hCG. In this study, the mechanism by which more oocytes are ovulated was investigated. Apoptoti...
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004225001063 |
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| author | Eri Ueno Mitsuya Watanabe Yoshiko Kondo Naomi Nakagata Toru Takeo Satohiro Nakao Katsueki Ogiwara |
| author_facet | Eri Ueno Mitsuya Watanabe Yoshiko Kondo Naomi Nakagata Toru Takeo Satohiro Nakao Katsueki Ogiwara |
| author_sort | Eri Ueno |
| collection | DOAJ |
| description | Summary: Mice administered an inhibin antiserum, equine chorionic gonadotropin (eCG) mixture called CARD HyperOva (OVA)/human chorionic gonadotropin (hCG), ovulate more oocytes than those administered eCG/hCG. In this study, the mechanism by which more oocytes are ovulated was investigated. Apoptotic cells were not observed in ovaries 24 and 48 h after OVA injection, and INHBA expression was absent in the growing follicles with apoptotic cells, whereas granulosa cells in follicles expressing INHBA also expressed estrogen receptor α (ESR1). ESR1 and CYP19A1 expression and 17β-estradiol (E2) in sera significantly increased in OVA-injected mice. Esr1 and Cyp19a1 expression and E2 concentration increased in ovaries cultured with activin A. The ovulation number increased in mice administered diethylstilbestrol. Taken together, these results suggest that ESR1 and E2 are involved in the inhibition of follicular atresia, which increases ovulation and oocyte number. This study may provide valuable information on the molecular mechanisms underlying mouse follicle selection. |
| format | Article |
| id | doaj-art-9fb516488db949ad90540ba0ccb9ec64 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-9fb516488db949ad90540ba0ccb9ec642025-02-02T05:29:10ZengElsevieriScience2589-00422025-02-0128211184617β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresiaEri Ueno0Mitsuya Watanabe1Yoshiko Kondo2Naomi Nakagata3Toru Takeo4Satohiro Nakao5Katsueki Ogiwara6Laboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanLaboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanLaboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo 060-0810, JapanDivision of Reproductive Biotechnology and Innovation, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, JapanDivision of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, JapanDivision of Reproductive Engineering, Center for Animal Resources and Development, Institute of Resource Development and Analysis, Kumamoto University, 2-2-1 Honjo, Chuo-ku, Kumamoto 860-0811, JapanLaboratory of Reproductive and Developmental Biology, Faculty of Science, Hokkaido University, Sapporo 060-0810, Japan; Corresponding authorSummary: Mice administered an inhibin antiserum, equine chorionic gonadotropin (eCG) mixture called CARD HyperOva (OVA)/human chorionic gonadotropin (hCG), ovulate more oocytes than those administered eCG/hCG. In this study, the mechanism by which more oocytes are ovulated was investigated. Apoptotic cells were not observed in ovaries 24 and 48 h after OVA injection, and INHBA expression was absent in the growing follicles with apoptotic cells, whereas granulosa cells in follicles expressing INHBA also expressed estrogen receptor α (ESR1). ESR1 and CYP19A1 expression and 17β-estradiol (E2) in sera significantly increased in OVA-injected mice. Esr1 and Cyp19a1 expression and E2 concentration increased in ovaries cultured with activin A. The ovulation number increased in mice administered diethylstilbestrol. Taken together, these results suggest that ESR1 and E2 are involved in the inhibition of follicular atresia, which increases ovulation and oocyte number. This study may provide valuable information on the molecular mechanisms underlying mouse follicle selection.http://www.sciencedirect.com/science/article/pii/S2589004225001063EndocrinologyRodent reproductionMolecular physiology |
| spellingShingle | Eri Ueno Mitsuya Watanabe Yoshiko Kondo Naomi Nakagata Toru Takeo Satohiro Nakao Katsueki Ogiwara 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia iScience Endocrinology Rodent reproduction Molecular physiology |
| title | 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| title_full | 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| title_fullStr | 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| title_full_unstemmed | 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| title_short | 17β-estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| title_sort | 17β estradiol and estrogen receptor alpha protect mouse ovarian follicle development by repressing atresia |
| topic | Endocrinology Rodent reproduction Molecular physiology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004225001063 |
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