<i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth

Background: In this work the plant growth-promoting (PGP) qualities of the <i>Enterobacter soli strain AF-22b-4245</i> were studied, including screening tests for PGP, whole genome sequencing (WGS) and genome annotation, and greenhouse experiments on wheat. A gene table was formed that a...

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Main Authors: Ekaterina Alexeevna Sokolova, Olga Viktorovna Mishukova, Inna Viktorovna Khlistun, Irina Nikolaevna Tromenschleger, Evgeniya Vladimirovna Chumanova, Elena Nikolaevna Voronina
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Microbiology Research
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Online Access:https://www.mdpi.com/2036-7481/16/2/34
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author Ekaterina Alexeevna Sokolova
Olga Viktorovna Mishukova
Inna Viktorovna Khlistun
Irina Nikolaevna Tromenschleger
Evgeniya Vladimirovna Chumanova
Elena Nikolaevna Voronina
author_facet Ekaterina Alexeevna Sokolova
Olga Viktorovna Mishukova
Inna Viktorovna Khlistun
Irina Nikolaevna Tromenschleger
Evgeniya Vladimirovna Chumanova
Elena Nikolaevna Voronina
author_sort Ekaterina Alexeevna Sokolova
collection DOAJ
description Background: In this work the plant growth-promoting (PGP) qualities of the <i>Enterobacter soli strain AF-22b-4245</i> were studied, including screening tests for PGP, whole genome sequencing (WGS) and genome annotation, and greenhouse experiments on wheat. A gene table was formed that allows us to evaluate the potential PGP properties of a microorganism based on the results of genome-wide sequencing. Results: Based on the results of screening tests and genome annotation, it can be concluded that the <i>E. soli strain AF-22b-4245</i> strain may have PGP properties, which consist in the ability to survive in arid and saline soils contaminated with copper, arsenic, lead, and chromium soils, form biofilms, produce phytohormones, siderophores, and solubilize phosphorus. Based on the results of experiments on wheat, the <i>E. soli strain AF-22b-4245</i> increases the efficiency of mineral fertilizers; this effect persists even in conditions of drought and excess salt. It has been shown that <i>E. soli A F22b-4245</i> can compensate for the lack of soluble phosphorus in the mineral fertilizer, probably by solubilizing insoluble forms in the soil.
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spelling doaj-art-9fb19c2152ec4061b2cb12a77d41f5e82025-08-20T03:12:09ZengMDPI AGMicrobiology Research2036-74812025-01-011623410.3390/microbiolres16020034<i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat GrowthEkaterina Alexeevna Sokolova0Olga Viktorovna Mishukova1Inna Viktorovna Khlistun2Irina Nikolaevna Tromenschleger3Evgeniya Vladimirovna Chumanova4Elena Nikolaevna Voronina5Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, RussiaBackground: In this work the plant growth-promoting (PGP) qualities of the <i>Enterobacter soli strain AF-22b-4245</i> were studied, including screening tests for PGP, whole genome sequencing (WGS) and genome annotation, and greenhouse experiments on wheat. A gene table was formed that allows us to evaluate the potential PGP properties of a microorganism based on the results of genome-wide sequencing. Results: Based on the results of screening tests and genome annotation, it can be concluded that the <i>E. soli strain AF-22b-4245</i> strain may have PGP properties, which consist in the ability to survive in arid and saline soils contaminated with copper, arsenic, lead, and chromium soils, form biofilms, produce phytohormones, siderophores, and solubilize phosphorus. Based on the results of experiments on wheat, the <i>E. soli strain AF-22b-4245</i> increases the efficiency of mineral fertilizers; this effect persists even in conditions of drought and excess salt. It has been shown that <i>E. soli A F22b-4245</i> can compensate for the lack of soluble phosphorus in the mineral fertilizer, probably by solubilizing insoluble forms in the soil.https://www.mdpi.com/2036-7481/16/2/34genome annotationarid soilssaline soilssolubility of phosphatesplant growth-promoting (PGP)whole genome sequencing (WGS)
spellingShingle Ekaterina Alexeevna Sokolova
Olga Viktorovna Mishukova
Inna Viktorovna Khlistun
Irina Nikolaevna Tromenschleger
Evgeniya Vladimirovna Chumanova
Elena Nikolaevna Voronina
<i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
Microbiology Research
genome annotation
arid soils
saline soils
solubility of phosphates
plant growth-promoting (PGP)
whole genome sequencing (WGS)
title <i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
title_full <i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
title_fullStr <i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
title_full_unstemmed <i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
title_short <i>Enterobacter soli Strain AF-22b-4245</i>: Study of the Genome and the Effect on Wheat Growth
title_sort i enterobacter soli strain af 22b 4245 i study of the genome and the effect on wheat growth
topic genome annotation
arid soils
saline soils
solubility of phosphates
plant growth-promoting (PGP)
whole genome sequencing (WGS)
url https://www.mdpi.com/2036-7481/16/2/34
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