Turnip mosaic virus in rhubarb grown on farms in Poland
The aim of this study was the detection and molecular characterization of newly identified turnip mosaic virus (TuMV) isolates infecting rhubarb in Poland. The presence of the virus in rhubarb was confirmed by ELISA and RT-PCR techniques. The specificity of the obtained products was verified by Sang...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Academy of Sciences
2025-07-01
|
| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/135804/PDF/280%E2%80%93285_OA_JPPR_65_2_2293_Komorowska.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850103843166093312 |
|---|---|
| author | Beata Komorowska Anna Jarecka-Boncela Magdalena Ptaszek |
| author_facet | Beata Komorowska Anna Jarecka-Boncela Magdalena Ptaszek |
| author_sort | Beata Komorowska |
| collection | DOAJ |
| description | The aim of this study was the detection and molecular characterization of newly identified turnip mosaic virus (TuMV) isolates infecting rhubarb in Poland. The presence of the virus in rhubarb was confirmed by ELISA and RT-PCR techniques. The specificity of the obtained products was verified by Sanger sequencing. Two sequences (1,077 nt) of the TuMV coat protein gene and the 3’-terminal non-coding region were uploaded to the Genbank database (access no. MG882689 and MG882690). The phylogenetic analysis was performed based on the coat protein gene sequences of two new Polish isolates from rhubarb and 43 other TuMV sequences retrieved from the Genbank. The isolates studied were grouped with an isolate from rhubarb (AB701709) found in the UK (98% nucleotide identity). It is the first phylogenetic analysis of TuMV isolates infecting rhubarb in Poland. |
| format | Article |
| id | doaj-art-9fb0527710804e5bae7e5682cfc1a6d2 |
| institution | DOAJ |
| issn | 1427-4345 1899-007X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj-art-9fb0527710804e5bae7e5682cfc1a6d22025-08-20T02:39:27ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2025-07-01vol. 65No 2https://doi.org/10.24425/jppr.2025.155056Turnip mosaic virus in rhubarb grown on farms in PolandBeata Komorowska0https://orcid.org/0000-0002-0093-3842Anna Jarecka-Boncela1https://orcid.org/0000-0002-0326-148XMagdalena Ptaszek2https://orcid.org/0000-0002-4738-3681Plant Protection, The National Institute of Horticultural Research, Skierniewice, PolandPlant Protection, The National Institute of Horticultural Research, Skierniewice, PolandPlant Protection, The National Institute of Horticultural Research, Skierniewice, PolandThe aim of this study was the detection and molecular characterization of newly identified turnip mosaic virus (TuMV) isolates infecting rhubarb in Poland. The presence of the virus in rhubarb was confirmed by ELISA and RT-PCR techniques. The specificity of the obtained products was verified by Sanger sequencing. Two sequences (1,077 nt) of the TuMV coat protein gene and the 3’-terminal non-coding region were uploaded to the Genbank database (access no. MG882689 and MG882690). The phylogenetic analysis was performed based on the coat protein gene sequences of two new Polish isolates from rhubarb and 43 other TuMV sequences retrieved from the Genbank. The isolates studied were grouped with an isolate from rhubarb (AB701709) found in the UK (98% nucleotide identity). It is the first phylogenetic analysis of TuMV isolates infecting rhubarb in Poland.https://journals.pan.pl/Content/135804/PDF/280%E2%80%93285_OA_JPPR_65_2_2293_Komorowska.pdfphylogenesisrheum rhabarbarumrt-pcrturnip mosaic virus (tumv) |
| spellingShingle | Beata Komorowska Anna Jarecka-Boncela Magdalena Ptaszek Turnip mosaic virus in rhubarb grown on farms in Poland Journal of Plant Protection Research phylogenesis rheum rhabarbarum rt-pcr turnip mosaic virus (tumv) |
| title | Turnip mosaic virus in rhubarb grown on farms in Poland |
| title_full | Turnip mosaic virus in rhubarb grown on farms in Poland |
| title_fullStr | Turnip mosaic virus in rhubarb grown on farms in Poland |
| title_full_unstemmed | Turnip mosaic virus in rhubarb grown on farms in Poland |
| title_short | Turnip mosaic virus in rhubarb grown on farms in Poland |
| title_sort | turnip mosaic virus in rhubarb grown on farms in poland |
| topic | phylogenesis rheum rhabarbarum rt-pcr turnip mosaic virus (tumv) |
| url | https://journals.pan.pl/Content/135804/PDF/280%E2%80%93285_OA_JPPR_65_2_2293_Komorowska.pdf |
| work_keys_str_mv | AT beatakomorowska turnipmosaicvirusinrhubarbgrownonfarmsinpoland AT annajareckaboncela turnipmosaicvirusinrhubarbgrownonfarmsinpoland AT magdalenaptaszek turnipmosaicvirusinrhubarbgrownonfarmsinpoland |