Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment

IntroductionIt is well acknowledged that lipids assume a critical role in oocyte maturation and early embryonic metabolism, this study aimed to evaluate the relationship between the lipid composition of plasma and follicular fluid (FF), and the consequences of embryonic development. This study compa...

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Main Authors: Yingxin Celia Jiang, Qi Che, Xinmei Lu, Miao Liu, Yao Ye, Xiang Cao, Xushuo Li, Yanxia Zhan, Xi Dong, Yunfeng Cheng, Christopher O’Neill
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Endocrinology
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Online Access:https://www.frontiersin.org/articles/10.3389/fendo.2024.1464171/full
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author Yingxin Celia Jiang
Yingxin Celia Jiang
Qi Che
Xinmei Lu
Miao Liu
Yao Ye
Xiang Cao
Xushuo Li
Yanxia Zhan
Xi Dong
Yunfeng Cheng
Yunfeng Cheng
Yunfeng Cheng
Christopher O’Neill
author_facet Yingxin Celia Jiang
Yingxin Celia Jiang
Qi Che
Xinmei Lu
Miao Liu
Yao Ye
Xiang Cao
Xushuo Li
Yanxia Zhan
Xi Dong
Yunfeng Cheng
Yunfeng Cheng
Yunfeng Cheng
Christopher O’Neill
author_sort Yingxin Celia Jiang
collection DOAJ
description IntroductionIt is well acknowledged that lipids assume a critical role in oocyte maturation and early embryonic metabolism, this study aimed to evaluate the relationship between the lipid composition of plasma and follicular fluid (FF), and the consequences of embryonic development. This study compared the lipidomic profiles of paired plasma and FF samples obtained from sixty-five Chinese women who underwent assisted reproductive technology (ART) treatments.MethodsNon-targeted lipidomics analysis.ResultResults not only indicated similarities in lipid composition between these biofluids, but also revealed a number of unique differences. The biomatrix distinction was found to be primarily driven by lipids belonging to the lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE), ether PE, and triglyceride (TG) classes. In addition, specific species from these subclasses were discovered to be correlated with embryo development outcomes during ART. Notably, the composition of the fatty acyl chains appeared to play a crucial role in these associations. Furthermore, thirteen plasma lipid variables were identified, represented by Phosphatidylcholine 18:014:0 and PE P-18:020:1, which correlated with successful blastocyst formation (BF).DiscussionThe present study demonstrated that FF has a distinctive lipid composition, setting it apart from plasma; and the association observed with embryonic development underscored an important role of lipid composition in the healthy development of oocytes.
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publishDate 2024-12-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Endocrinology
spelling doaj-art-9fa5ac9a8cd34759b40affe2f8cc36252025-08-20T01:57:45ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922024-12-011510.3389/fendo.2024.14641711464171Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatmentYingxin Celia Jiang0Yingxin Celia Jiang1Qi Che2Xinmei Lu3Miao Liu4Yao Ye5Xiang Cao6Xushuo Li7Yanxia Zhan8Xi Dong9Yunfeng Cheng10Yunfeng Cheng11Yunfeng Cheng12Christopher O’Neill13Charles Perkins Centre, The University of Sydney, Sydney, NSW, AustraliaCentenary Institute, The University of Sydney, Sydney, NSW, AustraliaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaCenter for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, ChinaDepartment of Hematology, Zhongshan Hospital, Fudan University, Shanghai, ChinaReproductive Medicine Centre, Zhongshan Hospital, Fudan University, Shanghai, ChinaCenter for Tumor Diagnosis & Therapy, Jinshan Hospital, Fudan University, Shanghai, ChinaDepartment of Hematology, Zhongshan Hospital, Fudan University, Shanghai, ChinaInstitute of Clinical Science, Zhongshan Hospital, Fudan University, Shanghai, ChinaWoolcock Institute for Medical Research, University of Technology, Sydney, NSW, AustraliaIntroductionIt is well acknowledged that lipids assume a critical role in oocyte maturation and early embryonic metabolism, this study aimed to evaluate the relationship between the lipid composition of plasma and follicular fluid (FF), and the consequences of embryonic development. This study compared the lipidomic profiles of paired plasma and FF samples obtained from sixty-five Chinese women who underwent assisted reproductive technology (ART) treatments.MethodsNon-targeted lipidomics analysis.ResultResults not only indicated similarities in lipid composition between these biofluids, but also revealed a number of unique differences. The biomatrix distinction was found to be primarily driven by lipids belonging to the lysophosphatidylcholines (LPC), phosphatidylethanolamines (PE), ether PE, and triglyceride (TG) classes. In addition, specific species from these subclasses were discovered to be correlated with embryo development outcomes during ART. Notably, the composition of the fatty acyl chains appeared to play a crucial role in these associations. Furthermore, thirteen plasma lipid variables were identified, represented by Phosphatidylcholine 18:014:0 and PE P-18:020:1, which correlated with successful blastocyst formation (BF).DiscussionThe present study demonstrated that FF has a distinctive lipid composition, setting it apart from plasma; and the association observed with embryonic development underscored an important role of lipid composition in the healthy development of oocytes.https://www.frontiersin.org/articles/10.3389/fendo.2024.1464171/fulllipidomicsblastocyst formationplasmafollicular fluidLC-MSoocyte developmental competence
spellingShingle Yingxin Celia Jiang
Yingxin Celia Jiang
Qi Che
Xinmei Lu
Miao Liu
Yao Ye
Xiang Cao
Xushuo Li
Yanxia Zhan
Xi Dong
Yunfeng Cheng
Yunfeng Cheng
Yunfeng Cheng
Christopher O’Neill
Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
Frontiers in Endocrinology
lipidomics
blastocyst formation
plasma
follicular fluid
LC-MS
oocyte developmental competence
title Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
title_full Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
title_fullStr Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
title_full_unstemmed Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
title_short Follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during ART treatment
title_sort follicular fluid and plasma lipidome profiling and associations towards embryonic development outcomes during art treatment
topic lipidomics
blastocyst formation
plasma
follicular fluid
LC-MS
oocyte developmental competence
url https://www.frontiersin.org/articles/10.3389/fendo.2024.1464171/full
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