One-week 96-well soft agar growth assay for cancer target validation
Soft agar growth, used to measure cell anchorage-independent proliferation potential, is one of the most important and most commonly used assays to detect cell transformation. However, the traditional soft agar assay is time-consuming, labor-intensive, and plagued with inconsistencies due to individ...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2004-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04365ST07 |
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| _version_ | 1850151801944276992 |
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| author | Ning Ke Aaron Albers Gisela Claassen De-hua Yu Jon E. Chatterton Xiuyuan Hu Bernd Meyhack Flossie Wong-Staal Qi-Xiang Li |
| author_facet | Ning Ke Aaron Albers Gisela Claassen De-hua Yu Jon E. Chatterton Xiuyuan Hu Bernd Meyhack Flossie Wong-Staal Qi-Xiang Li |
| author_sort | Ning Ke |
| collection | DOAJ |
| description | Soft agar growth, used to measure cell anchorage-independent proliferation potential, is one of the most important and most commonly used assays to detect cell transformation. However, the traditional soft agar assay is time-consuming, labor-intensive, and plagued with inconsistencies due to individual subjectivity. It does not, therefore, meet the increasing demands of today's oncology drug target screening or validation processes. This report describes an alternative 96-well soft agar growth assay that can function as a replacement for the traditional method and overcomes the aforementioned limitations. It offers the following advantages: a shortened assay duration (1 week instead of 4 weeks) that makes transient transfection or treatment possible; plate reader quantification of soft agar growth (measuring cloning efficiency and colony size); and a significant reduction in required labor. Higher throughput also makes it possible to process large numbers of samples and treatments simultaneously and in a much more efficient manner, while saving precious workspace and overall cost. |
| format | Article |
| id | doaj-art-9fa135bf8e21487ea30e62b1edc78b17 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2004-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-9fa135bf8e21487ea30e62b1edc78b172025-08-20T02:26:07ZengTaylor & Francis GroupBioTechniques0736-62051940-98182004-05-0136582683310.2144/04365ST07One-week 96-well soft agar growth assay for cancer target validationNing Ke0Aaron Albers1Gisela Claassen2De-hua Yu3Jon E. Chatterton4Xiuyuan Hu5Bernd Meyhack6Flossie Wong-Staal7Qi-Xiang Li81Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USA2Novartis Pharma AG, Basel, Switzerland1Immusol, Inc., San Diego, CA, USA1Immusol, Inc., San Diego, CA, USASoft agar growth, used to measure cell anchorage-independent proliferation potential, is one of the most important and most commonly used assays to detect cell transformation. However, the traditional soft agar assay is time-consuming, labor-intensive, and plagued with inconsistencies due to individual subjectivity. It does not, therefore, meet the increasing demands of today's oncology drug target screening or validation processes. This report describes an alternative 96-well soft agar growth assay that can function as a replacement for the traditional method and overcomes the aforementioned limitations. It offers the following advantages: a shortened assay duration (1 week instead of 4 weeks) that makes transient transfection or treatment possible; plate reader quantification of soft agar growth (measuring cloning efficiency and colony size); and a significant reduction in required labor. Higher throughput also makes it possible to process large numbers of samples and treatments simultaneously and in a much more efficient manner, while saving precious workspace and overall cost.https://www.future-science.com/doi/10.2144/04365ST07 |
| spellingShingle | Ning Ke Aaron Albers Gisela Claassen De-hua Yu Jon E. Chatterton Xiuyuan Hu Bernd Meyhack Flossie Wong-Staal Qi-Xiang Li One-week 96-well soft agar growth assay for cancer target validation BioTechniques |
| title | One-week 96-well soft agar growth assay for cancer target validation |
| title_full | One-week 96-well soft agar growth assay for cancer target validation |
| title_fullStr | One-week 96-well soft agar growth assay for cancer target validation |
| title_full_unstemmed | One-week 96-well soft agar growth assay for cancer target validation |
| title_short | One-week 96-well soft agar growth assay for cancer target validation |
| title_sort | one week 96 well soft agar growth assay for cancer target validation |
| url | https://www.future-science.com/doi/10.2144/04365ST07 |
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