Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life

ABSTRACT: There is a large and growing market for natural sweeteners with low glycemic index. Of particular interest are the “rare sugars” (e.g., d-tagatose). Rare sugars could be applied in dairy foods, such as yogurt, as sucrose replacements. Yogurt contains live and active cultures and, consequen...

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Main Authors: Kate Sorensen, Zeynep Atamer, Joy Waite-Cusic
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Journal of Dairy Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S0022030225000384
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author Kate Sorensen
Zeynep Atamer
Joy Waite-Cusic
author_facet Kate Sorensen
Zeynep Atamer
Joy Waite-Cusic
author_sort Kate Sorensen
collection DOAJ
description ABSTRACT: There is a large and growing market for natural sweeteners with low glycemic index. Of particular interest are the “rare sugars” (e.g., d-tagatose). Rare sugars could be applied in dairy foods, such as yogurt, as sucrose replacements. Yogurt contains live and active cultures and, consequently, their enzymes. These enzymes include those capable of sugar hydrolysis or isomerization, which could undermine the nutritional benefits of rare sugars. The purpose of this research was to evaluate sugar stability and influence of added sugars during storage of commercial nonfat Greek yogurts having simple and complex microbial compositions. Two commercial nonfat Greek yogurt products with different bacterial profiles (simple: Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus; complex: S. thermophilus, L. delbrueckii ssp. bulgaricus, Bifidobacterium lactis, Lactobacillus acidophilus, and Lacticaseibacillus rhamnosus) were supplemented with sugars (fructose, galactose, glucose, lactose, sucrose, and d-tagatose) at 10% (wt/wt) in duplicate and sampled every 2 wk. Individual sugar concentrations and lactic acid were measured using a combination of enzyme kits (Megazyme) and high-performance liquid chromatography. Sugar (lactose, glucose, galactose) and lactic acid isomer concentrations were significantly different between the 2 commercial products, but neither product changed significantly during refrigerated storage. Each added sugar (10%) was stable throughout the 6-wk refrigerated storage period. The pH of all yogurts decreased slightly (−0.1 pH) during storage, but with no corresponding change in lactic acid concentration. These results provide foundational data on the stability of various sugars in cultured dairy products throughout refrigerated shelf life.
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spelling doaj-art-9fa08f7ec10b4fe598fe25f69d36fe5c2025-08-20T02:11:58ZengElsevierJournal of Dairy Science0022-03022025-04-0110843247325710.3168/jds.2024-25667Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf lifeKate Sorensen0Zeynep Atamer1Joy Waite-Cusic2Department of Food Science and Technology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331Department of Food Science and Technology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331Corresponding author; Department of Food Science and Technology, College of Agricultural Sciences, Oregon State University, Corvallis, OR 97331ABSTRACT: There is a large and growing market for natural sweeteners with low glycemic index. Of particular interest are the “rare sugars” (e.g., d-tagatose). Rare sugars could be applied in dairy foods, such as yogurt, as sucrose replacements. Yogurt contains live and active cultures and, consequently, their enzymes. These enzymes include those capable of sugar hydrolysis or isomerization, which could undermine the nutritional benefits of rare sugars. The purpose of this research was to evaluate sugar stability and influence of added sugars during storage of commercial nonfat Greek yogurts having simple and complex microbial compositions. Two commercial nonfat Greek yogurt products with different bacterial profiles (simple: Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus; complex: S. thermophilus, L. delbrueckii ssp. bulgaricus, Bifidobacterium lactis, Lactobacillus acidophilus, and Lacticaseibacillus rhamnosus) were supplemented with sugars (fructose, galactose, glucose, lactose, sucrose, and d-tagatose) at 10% (wt/wt) in duplicate and sampled every 2 wk. Individual sugar concentrations and lactic acid were measured using a combination of enzyme kits (Megazyme) and high-performance liquid chromatography. Sugar (lactose, glucose, galactose) and lactic acid isomer concentrations were significantly different between the 2 commercial products, but neither product changed significantly during refrigerated storage. Each added sugar (10%) was stable throughout the 6-wk refrigerated storage period. The pH of all yogurts decreased slightly (−0.1 pH) during storage, but with no corresponding change in lactic acid concentration. These results provide foundational data on the stability of various sugars in cultured dairy products throughout refrigerated shelf life.http://www.sciencedirect.com/science/article/pii/S0022030225000384cultured dairy productsrare sugarslactoseshelf life
spellingShingle Kate Sorensen
Zeynep Atamer
Joy Waite-Cusic
Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
Journal of Dairy Science
cultured dairy products
rare sugars
lactose
shelf life
title Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
title_full Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
title_fullStr Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
title_full_unstemmed Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
title_short Stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
title_sort stability of sugars in yogurts with simple and complex microbial composition during refrigerated shelf life
topic cultured dairy products
rare sugars
lactose
shelf life
url http://www.sciencedirect.com/science/article/pii/S0022030225000384
work_keys_str_mv AT katesorensen stabilityofsugarsinyogurtswithsimpleandcomplexmicrobialcompositionduringrefrigeratedshelflife
AT zeynepatamer stabilityofsugarsinyogurtswithsimpleandcomplexmicrobialcompositionduringrefrigeratedshelflife
AT joywaitecusic stabilityofsugarsinyogurtswithsimpleandcomplexmicrobialcompositionduringrefrigeratedshelflife