Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen
This study evaluated aspects of the pollen viability of the species Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae), both native trees with ornamental, medicinal and industrial potential. The study used different concentrations of sucrose to determine pollen...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade do Estado de Santa Catarina
2024-09-01
|
| Series: | Revista de Ciências Agroveterinárias |
| Subjects: | |
| Online Access: | https://www.periodicos.udesc.br/index.php/agroveterinaria/article/view/24458 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850132108346916864 |
|---|---|
| author | Bruno Jan Schramm Corrêa Alexandra Cristina Schatz Sá Luciana Magda de Oliveira Adelar Mantovani Roseli Lopes da Costa Bortoluzzi |
| author_facet | Bruno Jan Schramm Corrêa Alexandra Cristina Schatz Sá Luciana Magda de Oliveira Adelar Mantovani Roseli Lopes da Costa Bortoluzzi |
| author_sort | Bruno Jan Schramm Corrêa |
| collection | DOAJ |
| description |
This study evaluated aspects of the pollen viability of the species Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae), both native trees with ornamental, medicinal and industrial potential. The study used different concentrations of sucrose to determine pollen viability in vitro and through colorimetric tests with specific dyes (Lugol, carmine acetic, and tetrazolium chloride – TTC – 0.1 and 0.05%). The two species also had their pollen stored for 30 and 90 days in freezers, refrigerators, and natural environments to check the best method for preserving their viability. The results showed that the highest average germination of M. euosma pollen was in a culture medium containing 30% sucrose. After 30 and 90 days of storage, pollen viability was significantly reduced, having preserved part of its potential only in the freezer. The pollen of S. reitzii had a higher average germination rate in a medium with 20% sucrose, but also showed a reduction in viability after 30 and 90 days of storage. The colorimetric tests showed that the pollen of both species responded to the dyes. However, the viability of Lugol and carmine acetic was considered to be overestimated in relation to the in vitro germination tests. Tetrazolium at 0.1% proved to be an alternative for checking the viability of S. reitzii pollen. It can be concluded that different concentrations of sucrose affect germination, and that storing pollen at a low temperature can preserve some of its viability in the short term.
|
| format | Article |
| id | doaj-art-9f9a0291ef78460dabd7ea0db24c106b |
| institution | OA Journals |
| issn | 2238-1171 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Universidade do Estado de Santa Catarina |
| record_format | Article |
| series | Revista de Ciências Agroveterinárias |
| spelling | doaj-art-9f9a0291ef78460dabd7ea0db24c106b2025-08-20T02:32:16ZengUniversidade do Estado de Santa CatarinaRevista de Ciências Agroveterinárias2238-11712024-09-0123210.5965/223811712322024203Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollenBruno Jan Schramm Corrêa0https://orcid.org/0000-0003-3528-4042Alexandra Cristina Schatz Sá1https://orcid.org/0000-0003-1532-8765Luciana Magda de Oliveira2https://orcid.org/0000-0001-7362-1041Adelar Mantovani3https://orcid.org/0000-0003-2952-2171Roseli Lopes da Costa Bortoluzzi4https://orcid.org/0000-0002-7445-7244Universidade do Estado de Santa Catarina Universidade do Estado de Santa Catarina Universidade do Estado de Santa Catarina Universidade do Estado de Santa Catarina Universidade do Estado de Santa Catarina This study evaluated aspects of the pollen viability of the species Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae), both native trees with ornamental, medicinal and industrial potential. The study used different concentrations of sucrose to determine pollen viability in vitro and through colorimetric tests with specific dyes (Lugol, carmine acetic, and tetrazolium chloride – TTC – 0.1 and 0.05%). The two species also had their pollen stored for 30 and 90 days in freezers, refrigerators, and natural environments to check the best method for preserving their viability. The results showed that the highest average germination of M. euosma pollen was in a culture medium containing 30% sucrose. After 30 and 90 days of storage, pollen viability was significantly reduced, having preserved part of its potential only in the freezer. The pollen of S. reitzii had a higher average germination rate in a medium with 20% sucrose, but also showed a reduction in viability after 30 and 90 days of storage. The colorimetric tests showed that the pollen of both species responded to the dyes. However, the viability of Lugol and carmine acetic was considered to be overestimated in relation to the in vitro germination tests. Tetrazolium at 0.1% proved to be an alternative for checking the viability of S. reitzii pollen. It can be concluded that different concentrations of sucrose affect germination, and that storing pollen at a low temperature can preserve some of its viability in the short term. https://www.periodicos.udesc.br/index.php/agroveterinaria/article/view/24458in vitro germinationtetrazoliumgrowth mediumfreezer |
| spellingShingle | Bruno Jan Schramm Corrêa Alexandra Cristina Schatz Sá Luciana Magda de Oliveira Adelar Mantovani Roseli Lopes da Costa Bortoluzzi Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen Revista de Ciências Agroveterinárias in vitro germination tetrazolium growth medium freezer |
| title | Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen |
| title_full | Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen |
| title_fullStr | Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen |
| title_full_unstemmed | Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen |
| title_short | Viability and storage of Myrceugenia euosma (O. Berg) D. Legrand and Siphoneugena reitzii D. Legrand (Myrtaceae) pollen |
| title_sort | viability and storage of myrceugenia euosma o berg d legrand and siphoneugena reitzii d legrand myrtaceae pollen |
| topic | in vitro germination tetrazolium growth medium freezer |
| url | https://www.periodicos.udesc.br/index.php/agroveterinaria/article/view/24458 |
| work_keys_str_mv | AT brunojanschrammcorrea viabilityandstorageofmyrceugeniaeuosmaobergdlegrandandsiphoneugenareitziidlegrandmyrtaceaepollen AT alexandracristinaschatzsa viabilityandstorageofmyrceugeniaeuosmaobergdlegrandandsiphoneugenareitziidlegrandmyrtaceaepollen AT lucianamagdadeoliveira viabilityandstorageofmyrceugeniaeuosmaobergdlegrandandsiphoneugenareitziidlegrandmyrtaceaepollen AT adelarmantovani viabilityandstorageofmyrceugeniaeuosmaobergdlegrandandsiphoneugenareitziidlegrandmyrtaceaepollen AT roselilopesdacostabortoluzzi viabilityandstorageofmyrceugeniaeuosmaobergdlegrandandsiphoneugenareitziidlegrandmyrtaceaepollen |