Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1

A strain of Ralstonia sp. FD-1 capable of using 4-fluoroaniline (4-FA) as the sole carbon and nitrogen source was investigated for its ability to utilize 4-FA isomers (2-FA, 3-FA) and homologs (2,4-DFA, 3,4-DFA, and 2,3,4-TFA). Both 4-FA and 3-FA could be mineralized as the sole carbon and nitrogen...

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Main Authors: Wenling Cao, Erxi Song, Dongsheng Shen, Meizhen Wang
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2015/206150
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author Wenling Cao
Erxi Song
Dongsheng Shen
Meizhen Wang
author_facet Wenling Cao
Erxi Song
Dongsheng Shen
Meizhen Wang
author_sort Wenling Cao
collection DOAJ
description A strain of Ralstonia sp. FD-1 capable of using 4-fluoroaniline (4-FA) as the sole carbon and nitrogen source was investigated for its ability to utilize 4-FA isomers (2-FA, 3-FA) and homologs (2,4-DFA, 3,4-DFA, and 2,3,4-TFA). Both 4-FA and 3-FA could be mineralized as the sole carbon and nitrogen source by FD-1. 2-FA, 2,4-DFA, 3,4-DFA, and 2,3,4-TFA could not be degraded by FD-1, respectively, and were selected as secondary substrates for cometabolism with 500 mg/L 4-FA as growth substrate. Bacterial growth (OD600), F− concentrations, and fluoroanilines contents were measured to determinate the degradation ability of 4-FA isomers and homologs by FD-1. FD-1 growth was inhibited by 2,4-DFA, 3,4-DFA, and 2,3,4-TFA at higher concentrations (400 mg/L), except for 2-FA. Complete fluoroanilines degradation was achieved while incomplete defluorination was characterized by the stoichiometric fluoride release indicating partial degradation but not total mineralization. When fluoroaniline was supplied to the resting cells of strain FD-1, a relatively effective removal was showed. Strain FD-1 had broadened application prospect of toxicity and low nutrition fluoroanilines wastewater.
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spelling doaj-art-9f99b0f9fd4647c4890403c05f355f8e2025-08-20T03:38:30ZengWileyJournal of Chemistry2090-90632090-90712015-01-01201510.1155/2015/206150206150Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1Wenling Cao0Erxi Song1Dongsheng Shen2Meizhen Wang3Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, ChinaZhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, ChinaZhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, ChinaZhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling, College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, ChinaA strain of Ralstonia sp. FD-1 capable of using 4-fluoroaniline (4-FA) as the sole carbon and nitrogen source was investigated for its ability to utilize 4-FA isomers (2-FA, 3-FA) and homologs (2,4-DFA, 3,4-DFA, and 2,3,4-TFA). Both 4-FA and 3-FA could be mineralized as the sole carbon and nitrogen source by FD-1. 2-FA, 2,4-DFA, 3,4-DFA, and 2,3,4-TFA could not be degraded by FD-1, respectively, and were selected as secondary substrates for cometabolism with 500 mg/L 4-FA as growth substrate. Bacterial growth (OD600), F− concentrations, and fluoroanilines contents were measured to determinate the degradation ability of 4-FA isomers and homologs by FD-1. FD-1 growth was inhibited by 2,4-DFA, 3,4-DFA, and 2,3,4-TFA at higher concentrations (400 mg/L), except for 2-FA. Complete fluoroanilines degradation was achieved while incomplete defluorination was characterized by the stoichiometric fluoride release indicating partial degradation but not total mineralization. When fluoroaniline was supplied to the resting cells of strain FD-1, a relatively effective removal was showed. Strain FD-1 had broadened application prospect of toxicity and low nutrition fluoroanilines wastewater.http://dx.doi.org/10.1155/2015/206150
spellingShingle Wenling Cao
Erxi Song
Dongsheng Shen
Meizhen Wang
Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
Journal of Chemistry
title Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
title_full Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
title_fullStr Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
title_full_unstemmed Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
title_short Cometabolism of Fluoroanilines in the Presence of 4-Fluoroaniline by Ralstonia sp. FD-1
title_sort cometabolism of fluoroanilines in the presence of 4 fluoroaniline by ralstonia sp fd 1
url http://dx.doi.org/10.1155/2015/206150
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