Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.

Brucellosis and Q-fever are two highly contagious bacterial diseases with significant zoonotic potential and economic threats, yet they often remain underreported and neglected in low- and middle-income countries. The present study aimed to determine the seroprevalence of Brucellosis and Q-fever in...

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Main Authors: Dharanya Muthiah, Mahavir Singh, Rajesh Chhabra, Renu Gupta, Swati Dahiya, Priya, Jinu Manoj
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2024-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0314726
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author Dharanya Muthiah
Mahavir Singh
Rajesh Chhabra
Renu Gupta
Swati Dahiya
Priya
Jinu Manoj
author_facet Dharanya Muthiah
Mahavir Singh
Rajesh Chhabra
Renu Gupta
Swati Dahiya
Priya
Jinu Manoj
author_sort Dharanya Muthiah
collection DOAJ
description Brucellosis and Q-fever are two highly contagious bacterial diseases with significant zoonotic potential and economic threats, yet they often remain underreported and neglected in low- and middle-income countries. The present study aimed to determine the seroprevalence of Brucellosis and Q-fever in water buffaloes in the Haryana state of India to implement effective preventive measures for disease control. The study covered all 22 districts of Haryana and involved 400 serum samples collected from female buffaloes belonging to two age groups and three distinct agro-climatic zones. The collected sera were tested using the Rose Bengal Plate agglutination test (RBPT) and commercial indirect enzyme linked immunosorbent assay (i-ELISA) for the presence of antibodies against smooth strains of Brucella spp. Additionally, the same serum samples were examined by i-ELISA for antibodies against Coxiella burnetii. The overall seroprevalence of brucellosis was observed as 8.25% (CI: 5.75-11.39) using the RBPT and 7.5% (CI: 5.12-10.53) by i-ELISA. The overall observed seroprevalence of Q-fever was 2.00% (CI: 0.87-3.90). No significant variation was seen in seropositivity of both diseases based on age and agroclimatic zones of the state. The findings of this study provide critical insights to farmers, agricultural organizations, veterinary services, and healthcare providers, facilitating more effective implementation of disease control measures.
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spelling doaj-art-9f8e545231194ddaa2ea305e6e4feead2025-08-20T02:38:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011912e031472610.1371/journal.pone.0314726Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.Dharanya MuthiahMahavir SinghRajesh ChhabraRenu GuptaSwati DahiyaPriyaJinu ManojBrucellosis and Q-fever are two highly contagious bacterial diseases with significant zoonotic potential and economic threats, yet they often remain underreported and neglected in low- and middle-income countries. The present study aimed to determine the seroprevalence of Brucellosis and Q-fever in water buffaloes in the Haryana state of India to implement effective preventive measures for disease control. The study covered all 22 districts of Haryana and involved 400 serum samples collected from female buffaloes belonging to two age groups and three distinct agro-climatic zones. The collected sera were tested using the Rose Bengal Plate agglutination test (RBPT) and commercial indirect enzyme linked immunosorbent assay (i-ELISA) for the presence of antibodies against smooth strains of Brucella spp. Additionally, the same serum samples were examined by i-ELISA for antibodies against Coxiella burnetii. The overall seroprevalence of brucellosis was observed as 8.25% (CI: 5.75-11.39) using the RBPT and 7.5% (CI: 5.12-10.53) by i-ELISA. The overall observed seroprevalence of Q-fever was 2.00% (CI: 0.87-3.90). No significant variation was seen in seropositivity of both diseases based on age and agroclimatic zones of the state. The findings of this study provide critical insights to farmers, agricultural organizations, veterinary services, and healthcare providers, facilitating more effective implementation of disease control measures.https://doi.org/10.1371/journal.pone.0314726
spellingShingle Dharanya Muthiah
Mahavir Singh
Rajesh Chhabra
Renu Gupta
Swati Dahiya
Priya
Jinu Manoj
Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
PLoS ONE
title Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
title_full Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
title_fullStr Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
title_full_unstemmed Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
title_short Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India.
title_sort seroprevalance of brucellosis and q fever in water buffaloes bubalus bubalis in haryana state india
url https://doi.org/10.1371/journal.pone.0314726
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