Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa
IntroductionIncreasing the research on the development and utilization of unconventional feed resources is one of the effective ways for the sustainable development of herbivorous animal husbandry. China is one of the countries most severely impacted by the invasion of the alien plant Solanum rostra...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiomes |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1510774/full |
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| author | Yuyu Li Yuyu Li Hua Wang Hua Wang Yandong Zhang Yu Ji Yu Ji Lizhu Guo Lizhu Guo Lifen Hao Lifen Hao Kejian Lin Kejian Lin |
| author_facet | Yuyu Li Yuyu Li Hua Wang Hua Wang Yandong Zhang Yu Ji Yu Ji Lizhu Guo Lizhu Guo Lifen Hao Lifen Hao Kejian Lin Kejian Lin |
| author_sort | Yuyu Li |
| collection | DOAJ |
| description | IntroductionIncreasing the research on the development and utilization of unconventional feed resources is one of the effective ways for the sustainable development of herbivorous animal husbandry. China is one of the countries most severely impacted by the invasion of the alien plant Solanum rostratum Dunal (S. rostratum), but this resource has not been used effectively.MethodsThe aim of this study was to investigate the effects of Lactobacillus plantarum and cellulase on the fermentation quality and microbial community in mixed silage of S. rostratum and alfalfa. Treatments were a control treatment with no additive (CK), Lactobacillus plantarum (LP), cellulase (CE), and Lactobacillus plantarum in combination with cellulase (L+C), all of which were stored at ambient temperature for 7, 15, 30, and 60 days.ResultsThe results showed that the mixture could retain dry matter (DM), crude protein (CP), and water soluble carbohydrates (WSC) content, increase lactic acid (LA) content, decrease pH and alkaloid content, and improve fermentation quality during silage. The use of additives increased the abundance of Lactobacillus and Weissella, which was related to the improvement of the quality of mixed silage and the degradation of total alkaloids. Differential microbial functions were mainly carbohydrate metabolism, biosynthesis of secondary metabolites and carbon metabolism.ConclusionThe application of additives and mixed silage provides a new idea for the feed utilization of S. rostratum. |
| format | Article |
| id | doaj-art-9f8c866ca6584950b287fb0bf5860df8 |
| institution | DOAJ |
| issn | 2813-4338 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiomes |
| spelling | doaj-art-9f8c866ca6584950b287fb0bf5860df82025-08-20T02:44:35ZengFrontiers Media S.A.Frontiers in Microbiomes2813-43382025-01-01310.3389/frmbi.2024.15107741510774Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfaYuyu Li0Yuyu Li1Hua Wang2Hua Wang3Yandong Zhang4Yu Ji5Yu Ji6Lizhu Guo7Lizhu Guo8Lifen Hao9Lifen Hao10Kejian Lin11Kejian Lin12Institute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaInstitute of Grassland Research, Chinese Academy of Agricultural Science, Hohhot, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Hohhot, ChinaIntroductionIncreasing the research on the development and utilization of unconventional feed resources is one of the effective ways for the sustainable development of herbivorous animal husbandry. China is one of the countries most severely impacted by the invasion of the alien plant Solanum rostratum Dunal (S. rostratum), but this resource has not been used effectively.MethodsThe aim of this study was to investigate the effects of Lactobacillus plantarum and cellulase on the fermentation quality and microbial community in mixed silage of S. rostratum and alfalfa. Treatments were a control treatment with no additive (CK), Lactobacillus plantarum (LP), cellulase (CE), and Lactobacillus plantarum in combination with cellulase (L+C), all of which were stored at ambient temperature for 7, 15, 30, and 60 days.ResultsThe results showed that the mixture could retain dry matter (DM), crude protein (CP), and water soluble carbohydrates (WSC) content, increase lactic acid (LA) content, decrease pH and alkaloid content, and improve fermentation quality during silage. The use of additives increased the abundance of Lactobacillus and Weissella, which was related to the improvement of the quality of mixed silage and the degradation of total alkaloids. Differential microbial functions were mainly carbohydrate metabolism, biosynthesis of secondary metabolites and carbon metabolism.ConclusionThe application of additives and mixed silage provides a new idea for the feed utilization of S. rostratum.https://www.frontiersin.org/articles/10.3389/frmbi.2024.1510774/fullSolanum rostratumalfalfamixed silageadditivesbacterial community |
| spellingShingle | Yuyu Li Yuyu Li Hua Wang Hua Wang Yandong Zhang Yu Ji Yu Ji Lizhu Guo Lizhu Guo Lifen Hao Lifen Hao Kejian Lin Kejian Lin Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa Frontiers in Microbiomes Solanum rostratum alfalfa mixed silage additives bacterial community |
| title | Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa |
| title_full | Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa |
| title_fullStr | Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa |
| title_full_unstemmed | Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa |
| title_short | Influence of Lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of Solanum rostratum and alfalfa |
| title_sort | influence of lactobacillus plantarum and cellulase on fermentation quality and microbial community in mixed silage of solanum rostratum and alfalfa |
| topic | Solanum rostratum alfalfa mixed silage additives bacterial community |
| url | https://www.frontiersin.org/articles/10.3389/frmbi.2024.1510774/full |
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