Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter
Aloin, a kind of active phenolic component, is sourced from <i>Aloe vera</i>. Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin prot...
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MDPI AG
2025-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/15/7/1052 |
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| author | Guo Wei Chuanliang Wang Rui Wang Peng Zhang Xuhui Geng Jinhua Li Abbas Ostovan Lingxin Chen Zhihua Song |
| author_facet | Guo Wei Chuanliang Wang Rui Wang Peng Zhang Xuhui Geng Jinhua Li Abbas Ostovan Lingxin Chen Zhihua Song |
| author_sort | Guo Wei |
| collection | DOAJ |
| description | Aloin, a kind of active phenolic component, is sourced from <i>Aloe vera</i>. Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin protection activities) and painful side effects (increased carcinogenicity caused by excessive use of aloin) impacting human health. This investigation was inspired by the good fluorescence properties of carbon dots (CDs); CD-based sensors have aroused a great deal of interest due to their excellent sensitivity and selectivity. Thus, it is of great significance to develop novel CD-based sensors for aloin determination. Herein, N,F-CDs were designed and synthesized through a convenient hydrothermal strategy; the synthesized N,F-CDs possessed good fluorescence performance and a small particle size (near 4.3 nm), which demonstrated the successful preparation of N,F-CDs. The resulting N,F-CDs possessed a large Stokes shift and could emit a highly stable green fluorescence. The fluorescence of the N,F-CDs could be effectively quenched by aloin through the inner filter effect. Furthermore, the synthesis procedure was easy to operate. Finally, the N,F-CD-coated test strips were fabricated and combined with a miniaturized fluorimeter for the fluorescence detection of aloin via the inner filter effect for the first time. The N,F-CD-coated test strips were fabricated and used for the fluorescence sensing of aloin, and the results were compared with a typical ultraviolet (UV) method. The N,F-CD-coated test strips exhibited high recovery (96.9~106.1%) and sensitivity (31.8 nM, n = 3), good selectivity, low sample consumption (1 μL), high speed (5 min), good stability, and anti-interference properties. The results indicate that N,F-CD-coated test strips are applicable for the quantitative determination of aloin in bovine serum, orange juice, and urine samples. |
| format | Article |
| id | doaj-art-9f83e4791d4f440fa5ff4c01ef1a8a2b |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-9f83e4791d4f440fa5ff4c01ef1a8a2b2025-08-20T02:45:37ZengMDPI AGBiomolecules2218-273X2025-07-01157105210.3390/biom15071052Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized FluorimeterGuo Wei0Chuanliang Wang1Rui Wang2Peng Zhang3Xuhui Geng4Jinhua Li5Abbas Ostovan6Lingxin Chen7Zhihua Song8School of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaCAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaCAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaCAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Center for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, ChinaSchool of Pharmacy, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation (Yantai University), Ministry of Education, Yantai University, Yantai 264005, ChinaAloin, a kind of active phenolic component, is sourced from <i>Aloe vera</i>. Recently, the determination of aloin has received enormous attention, owing to its positive performance (including anti-tumor, antibacterial, detoxification, liver protection, anti-stomach damage, and skin protection activities) and painful side effects (increased carcinogenicity caused by excessive use of aloin) impacting human health. This investigation was inspired by the good fluorescence properties of carbon dots (CDs); CD-based sensors have aroused a great deal of interest due to their excellent sensitivity and selectivity. Thus, it is of great significance to develop novel CD-based sensors for aloin determination. Herein, N,F-CDs were designed and synthesized through a convenient hydrothermal strategy; the synthesized N,F-CDs possessed good fluorescence performance and a small particle size (near 4.3 nm), which demonstrated the successful preparation of N,F-CDs. The resulting N,F-CDs possessed a large Stokes shift and could emit a highly stable green fluorescence. The fluorescence of the N,F-CDs could be effectively quenched by aloin through the inner filter effect. Furthermore, the synthesis procedure was easy to operate. Finally, the N,F-CD-coated test strips were fabricated and combined with a miniaturized fluorimeter for the fluorescence detection of aloin via the inner filter effect for the first time. The N,F-CD-coated test strips were fabricated and used for the fluorescence sensing of aloin, and the results were compared with a typical ultraviolet (UV) method. The N,F-CD-coated test strips exhibited high recovery (96.9~106.1%) and sensitivity (31.8 nM, n = 3), good selectivity, low sample consumption (1 μL), high speed (5 min), good stability, and anti-interference properties. The results indicate that N,F-CD-coated test strips are applicable for the quantitative determination of aloin in bovine serum, orange juice, and urine samples.https://www.mdpi.com/2218-273X/15/7/1052aloinnitrogen and fluorine-doped carbon dots (N,F-CDs)test stripsfluorescence analysisreal samples |
| spellingShingle | Guo Wei Chuanliang Wang Rui Wang Peng Zhang Xuhui Geng Jinhua Li Abbas Ostovan Lingxin Chen Zhihua Song Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter Biomolecules aloin nitrogen and fluorine-doped carbon dots (N,F-CDs) test strips fluorescence analysis real samples |
| title | Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter |
| title_full | Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter |
| title_fullStr | Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter |
| title_full_unstemmed | Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter |
| title_short | Sensitive and Facile Detection of Aloin via N,F-CD-Coated Test Strips Coupled with a Miniaturized Fluorimeter |
| title_sort | sensitive and facile detection of aloin via n f cd coated test strips coupled with a miniaturized fluorimeter |
| topic | aloin nitrogen and fluorine-doped carbon dots (N,F-CDs) test strips fluorescence analysis real samples |
| url | https://www.mdpi.com/2218-273X/15/7/1052 |
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