Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections
Tick-borne encephalitis is a flavivirus disease, endemic to vast areas of Europe and Asia, caused by the tick-borne encephalitis virus from the genus Orthoflavivirus, transmitted through the bite of a tick. The course of infection is characterized by fever followed by the development of potentially...
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| Format: | Article |
| Language: | Russian |
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Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”
2024-10-01
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| Series: | Проблемы особо опасных инфекций |
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| Online Access: | https://journal.microbe.ru/jour/article/view/2054 |
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| author | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko D. V. Shan’shin D. N. Shcherbakov |
| author_facet | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko D. V. Shan’shin D. N. Shcherbakov |
| author_sort | A. G. Poltavchenko |
| collection | DOAJ |
| description | Tick-borne encephalitis is a flavivirus disease, endemic to vast areas of Europe and Asia, caused by the tick-borne encephalitis virus from the genus Orthoflavivirus, transmitted through the bite of a tick. The course of infection is characterized by fever followed by the development of potentially fatal encephalitis. Serological diagnosis of tick-borne encephalitis is complicated by severe cross-reactivity with antibodies to other endemic and imported flavivirus infections. The aim of the study was to obtain a recombinant analogue of domain III of the surface protein E of the tick-borne encephalitis virus (EDIII-TBEV) and assess its ability to detect antibodies to the tick-borne encephalitis virus and differentiate them from antibodies to other flavivirus infections. Materials and methods. A comparative study was performed using panels of sera containing and not containing antibodies to the tick-borne encephalitis virus, and panels of samples with antibodies to yellow fever, dengue, and Zika viruses. The study was conducted using commercial tests and experimental kits based on EDIII-TBEV in the ELISA format and in the dot analysis format. Results and discussion. It has been shown that the use of experimental kits based on EDIII-TBEV makes it possible to effectively detect IgG antibodies to tick-borne encephalitis virus and, in contrast to commercial tests, to differentiate them from antibodies to the pathogens of dengue, yellow and Zika tropical fevers. A recombinant analogue of domain III of the surface protein E in the tick-borne encephalitis virus can be a useful capture reagent when creating diagnostic test systems in the format of enzyme-linked immunosorbent assay and dot-immunoassay for the differentiated detection of IgG antibodies to the tick-borne encephalitis virus. |
| format | Article |
| id | doaj-art-9f7e24ee25fa478f9b9d7813cd593ca8 |
| institution | Kabale University |
| issn | 0370-1069 2658-719X |
| language | Russian |
| publishDate | 2024-10-01 |
| publisher | Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” |
| record_format | Article |
| series | Проблемы особо опасных инфекций |
| spelling | doaj-art-9f7e24ee25fa478f9b9d7813cd593ca82025-08-20T03:50:11ZrusFederal Government Health Institution, Russian Research Anti-Plague Institute “Microbe”Проблемы особо опасных инфекций0370-10692658-719X2024-10-010315416210.21055/0370-1069-2024-3-154-1621587Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus InfectionsA. G. Poltavchenko0A. V. Ersh1P. V. Filatov2N. D. Ushkalenko3D. V. Shan’shin4D. N. Shcherbakov5State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”State Scientific Center of Virology and Biotechnology “Vector”Tick-borne encephalitis is a flavivirus disease, endemic to vast areas of Europe and Asia, caused by the tick-borne encephalitis virus from the genus Orthoflavivirus, transmitted through the bite of a tick. The course of infection is characterized by fever followed by the development of potentially fatal encephalitis. Serological diagnosis of tick-borne encephalitis is complicated by severe cross-reactivity with antibodies to other endemic and imported flavivirus infections. The aim of the study was to obtain a recombinant analogue of domain III of the surface protein E of the tick-borne encephalitis virus (EDIII-TBEV) and assess its ability to detect antibodies to the tick-borne encephalitis virus and differentiate them from antibodies to other flavivirus infections. Materials and methods. A comparative study was performed using panels of sera containing and not containing antibodies to the tick-borne encephalitis virus, and panels of samples with antibodies to yellow fever, dengue, and Zika viruses. The study was conducted using commercial tests and experimental kits based on EDIII-TBEV in the ELISA format and in the dot analysis format. Results and discussion. It has been shown that the use of experimental kits based on EDIII-TBEV makes it possible to effectively detect IgG antibodies to tick-borne encephalitis virus and, in contrast to commercial tests, to differentiate them from antibodies to the pathogens of dengue, yellow and Zika tropical fevers. A recombinant analogue of domain III of the surface protein E in the tick-borne encephalitis virus can be a useful capture reagent when creating diagnostic test systems in the format of enzyme-linked immunosorbent assay and dot-immunoassay for the differentiated detection of IgG antibodies to the tick-borne encephalitis virus.https://journal.microbe.ru/jour/article/view/2054glycoprotein etick-borne encephalitis virusrecombinant proteinsserological markerselisadot immunoassay |
| spellingShingle | A. G. Poltavchenko A. V. Ersh P. V. Filatov N. D. Ushkalenko D. V. Shan’shin D. N. Shcherbakov Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections Проблемы особо опасных инфекций glycoprotein e tick-borne encephalitis virus recombinant proteins serological markers elisa dot immunoassay |
| title | Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections |
| title_full | Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections |
| title_fullStr | Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections |
| title_full_unstemmed | Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections |
| title_short | Recombinant Analogue of Envelope Glycoprotein Domain III of the Tick-Borne Encephalitis Virus and its Application for the Differential Diagnosis of Flavivirus Infections |
| title_sort | recombinant analogue of envelope glycoprotein domain iii of the tick borne encephalitis virus and its application for the differential diagnosis of flavivirus infections |
| topic | glycoprotein e tick-borne encephalitis virus recombinant proteins serological markers elisa dot immunoassay |
| url | https://journal.microbe.ru/jour/article/view/2054 |
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