Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum
Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant var...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1516963/full |
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| _version_ | 1823861047356817408 |
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| author | Bingjie Li Bingjie Li Chen Chen Chen Chen Mengmeng Cui Yuhe Sun Jing Lv Changbo Dai |
| author_facet | Bingjie Li Bingjie Li Chen Chen Chen Chen Mengmeng Cui Yuhe Sun Jing Lv Changbo Dai |
| author_sort | Bingjie Li |
| collection | DOAJ |
| description | Glyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management. |
| format | Article |
| id | doaj-art-9f77efe92a854dc4a11e4c78d9cf689e |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-9f77efe92a854dc4a11e4c78d9cf689e2025-02-10T06:48:27ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011610.3389/fpls.2025.15169631516963Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacumBingjie Li0Bingjie Li1Chen Chen2Chen Chen3Mengmeng Cui4Yuhe Sun5Jing Lv6Changbo Dai7Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaGraduate School of Chinese Academy of Agricultural Sciences, Beijing, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaGraduate School of Chinese Academy of Agricultural Sciences, Beijing, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaTobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, ChinaGlyphosate is a widely used non-selective, broad-spectrum, systemic herbicide by interfering with the biosynthesis of aromatic amino acids. However, the emergence of glyphosate-resistant weeds has driven the need for enhanced herbicide resistance in crops. In this study, we engineered two mutant variants of the tobacco EPSPS gene through amino acid substitution (TIPS-NtEPSPS and P180S-NtEPSPS). These mutated EPSPS genes were overexpressed in tobacco under the control of CaMV35S promoters. Our results demonstrate that overexpression of TIPS-NtEPSPS significantly enhances glyphosate tolerance, allowing plants to withstand up to four times the recommended dose without compromising their fitness. This research highlights the potential of the TIPS-NtEPSPS mutant to improve herbicide resistance in tobacco, offering a viable approach for effective weed management.https://www.frontiersin.org/articles/10.3389/fpls.2025.1516963/fullglyphosateherbicide resistanceEPSP synthasetobaccogene editing |
| spellingShingle | Bingjie Li Bingjie Li Chen Chen Chen Chen Mengmeng Cui Yuhe Sun Jing Lv Changbo Dai Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum Frontiers in Plant Science glyphosate herbicide resistance EPSP synthase tobacco gene editing |
| title | Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum |
| title_full | Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum |
| title_fullStr | Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum |
| title_full_unstemmed | Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum |
| title_short | Exploring the potential role of EPSPS mutations for enhanced glyphosate resistance in Nicotiana tabacum |
| title_sort | exploring the potential role of epsps mutations for enhanced glyphosate resistance in nicotiana tabacum |
| topic | glyphosate herbicide resistance EPSP synthase tobacco gene editing |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1516963/full |
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