Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria
The current study aimed to optimize the production of cellulase and chitinase enzymes by plant growth promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) an...
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2020-02-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_73437_150d71f0be1f789aaa31b01224b6385c.pdf |
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| author | Alaa Fathalla Mohammed |
| author_facet | Alaa Fathalla Mohammed |
| author_sort | Alaa Fathalla Mohammed |
| collection | DOAJ |
| description | The current study aimed to optimize the production of cellulase and chitinase enzymes by plant growth
promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens
NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) and Bacillus
megaterium NBRC 15308 (TF2). The effect of different growth parameters including; pH, temperature, carbon
and nitrogen sources, and incubation periods were optimized for production of cellulase and chitinase enzymes by
the selected bacterial strains. Three strains mainly, B. subtilis SBMP4, S. liquefaciens ATCC 27592 and P.
fluorescens NBRC) recorded positive results for cellulase production. B. subtilis SBMP4 (EF1) and S.
liquefaciens ATCC 27592 (EW1) strains demonstrated significant ability to produce cellulase at 40°C, while P.
fluorescens NBRC (KW1) strain showed the maximum enzyme production at 30°C. Carboxymethyl cellulose
gave the highest cellulase production compared to the other carbon sources. Chitinase enzyme was optimally
produced by B. subtilis SBMP4 and S. liquefaciens ATCC 27592 strains under primary screening. B. subtilis
SBMP4 had the strongest ability to produce chitinase at 40oC, while S. liquefaciens ATCC 27592 at 30oC. The
optimum pH observed was at pH 6.0 to get the maximum chitinase production by S. liquefaciens ATCC 27592.
Potassium nitrate (KNO3) as an inorganic nitrogen source presented the highest chitinase production by B. subtilis
SBMP4, whereas yeast extract demonstrated significant chitinase production by S. liquefaciens ATCC 27592. |
| format | Article |
| id | doaj-art-9f77e22518ff4e89a3522d89103fd491 |
| institution | DOAJ |
| issn | 2537-0286 2537-0294 |
| language | English |
| publishDate | 2020-02-01 |
| publisher | ResearchersLinks, Ltd |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj-art-9f77e22518ff4e89a3522d89103fd4912025-08-20T03:18:55ZengResearchersLinks, LtdNovel Research in Microbiology Journal2537-02862537-02942020-02-014164165210.21608/nrmj.2020.73437Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteriaAlaa Fathalla Mohammed0Department of Agriculture Botany, Faculty of Agriculture, Suez Canal University, Ismailia, EgyptThe current study aimed to optimize the production of cellulase and chitinase enzymes by plant growth promoting rhizobacteria (PGPR). Bacteria were pre-isolated from soil and identified as; Pseudomonas fluorescens NBRC (KW1), Serratia liquefaciens ATCC 27592 (EW1), Bacillus subtilis SBMP4 (EF1) and Bacillus megaterium NBRC 15308 (TF2). The effect of different growth parameters including; pH, temperature, carbon and nitrogen sources, and incubation periods were optimized for production of cellulase and chitinase enzymes by the selected bacterial strains. Three strains mainly, B. subtilis SBMP4, S. liquefaciens ATCC 27592 and P. fluorescens NBRC) recorded positive results for cellulase production. B. subtilis SBMP4 (EF1) and S. liquefaciens ATCC 27592 (EW1) strains demonstrated significant ability to produce cellulase at 40°C, while P. fluorescens NBRC (KW1) strain showed the maximum enzyme production at 30°C. Carboxymethyl cellulose gave the highest cellulase production compared to the other carbon sources. Chitinase enzyme was optimally produced by B. subtilis SBMP4 and S. liquefaciens ATCC 27592 strains under primary screening. B. subtilis SBMP4 had the strongest ability to produce chitinase at 40oC, while S. liquefaciens ATCC 27592 at 30oC. The optimum pH observed was at pH 6.0 to get the maximum chitinase production by S. liquefaciens ATCC 27592. Potassium nitrate (KNO3) as an inorganic nitrogen source presented the highest chitinase production by B. subtilis SBMP4, whereas yeast extract demonstrated significant chitinase production by S. liquefaciens ATCC 27592.https://nrmj.journals.ekb.eg/article_73437_150d71f0be1f789aaa31b01224b6385c.pdfpseudomonas sp.serratia sp.bacillus sp.chitinasecellulase |
| spellingShingle | Alaa Fathalla Mohammed Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria Novel Research in Microbiology Journal pseudomonas sp. serratia sp. bacillus sp. chitinase cellulase |
| title | Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| title_full | Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| title_fullStr | Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| title_full_unstemmed | Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| title_short | Optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| title_sort | optimization of cellulase and chitinase enzymes production by plant growth promoting rhizobacteria |
| topic | pseudomonas sp. serratia sp. bacillus sp. chitinase cellulase |
| url | https://nrmj.journals.ekb.eg/article_73437_150d71f0be1f789aaa31b01224b6385c.pdf |
| work_keys_str_mv | AT alaafathallamohammed optimizationofcellulaseandchitinaseenzymesproductionbyplantgrowthpromotingrhizobacteria |