Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer
DNA methylation, which is mediated by DNMTs, plays crucial roles in regulating gene expression and cell differentiation. In this study, we identified adipogenesis-related genes and analyzed their coexpression with DNMT isoforms in breast cancer samples from the TCGA dataset. Our findings revealed th...
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Komunitas Ilmuwan dan Profesional Muslim Indonesia
2025-07-01
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| Series: | Communications in Science and Technology |
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| Online Access: | https://cst.kipmi.or.id/journal/article/view/1678 |
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| author | Sandeep Mallya Sharath Mohan Bhat Sachin Naie Sumati Kulkarni Manjunath B Joshi Shama Prasada Kabekkodu |
| author_facet | Sandeep Mallya Sharath Mohan Bhat Sachin Naie Sumati Kulkarni Manjunath B Joshi Shama Prasada Kabekkodu |
| author_sort | Sandeep Mallya |
| collection | DOAJ |
| description | DNA methylation, which is mediated by DNMTs, plays crucial roles in regulating gene expression and cell differentiation. In this study, we identified adipogenesis-related genes and analyzed their coexpression with DNMT isoforms in breast cancer samples from the TCGA dataset. Our findings revealed that 114 genes were coexpressed with DNMTs, among which six genes, GATA3, IRS1, LPIN1, ME3, SREBF1, and STAT1, were significantly negatively correlated with methylation and expression levels, as determined using Spearman correlation with false discovery rate correction to account for multiple testing. The differential expression patterns of these genes across breast cancer subtypes and their associations with survival outcomes were examined. Specifically, ME3 and STAT1 showed distinct associations with survival outcomes, where high ME3 expression correlated with significantly better survival rates, whereas low STAT1 expression was associated with improved prognosis. ME3 expression was significantly elevated in tumors with high adipocyte enrichment, particularly in the luminal B subtype, suggesting a subtype-specific relationship between adipogenesis and tumor behavior. Conversely, STAT1 exhibited lower expression in samples with high adipocyte counts, reinforcing its role in the tumor microenvironment. These results underscore the importance of DNMT-mediated DNA methylation in adipogenesis and breast cancer. |
| format | Article |
| id | doaj-art-9f514680e30b427caf17d44106d9e0a5 |
| institution | DOAJ |
| issn | 2502-9258 2502-9266 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Komunitas Ilmuwan dan Profesional Muslim Indonesia |
| record_format | Article |
| series | Communications in Science and Technology |
| spelling | doaj-art-9f514680e30b427caf17d44106d9e0a52025-08-20T02:57:04ZengKomunitas Ilmuwan dan Profesional Muslim IndonesiaCommunications in Science and Technology2502-92582502-92662025-07-0110120120810.21924/cst.10.1.2025.16781678Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancerSandeep MallyaSharath Mohan Bhat0Sachin NaieSumati KulkarniManjunath B JoshiShama Prasada KabekkoduDepartment of Ageing Research, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, IndiaDNA methylation, which is mediated by DNMTs, plays crucial roles in regulating gene expression and cell differentiation. In this study, we identified adipogenesis-related genes and analyzed their coexpression with DNMT isoforms in breast cancer samples from the TCGA dataset. Our findings revealed that 114 genes were coexpressed with DNMTs, among which six genes, GATA3, IRS1, LPIN1, ME3, SREBF1, and STAT1, were significantly negatively correlated with methylation and expression levels, as determined using Spearman correlation with false discovery rate correction to account for multiple testing. The differential expression patterns of these genes across breast cancer subtypes and their associations with survival outcomes were examined. Specifically, ME3 and STAT1 showed distinct associations with survival outcomes, where high ME3 expression correlated with significantly better survival rates, whereas low STAT1 expression was associated with improved prognosis. ME3 expression was significantly elevated in tumors with high adipocyte enrichment, particularly in the luminal B subtype, suggesting a subtype-specific relationship between adipogenesis and tumor behavior. Conversely, STAT1 exhibited lower expression in samples with high adipocyte counts, reinforcing its role in the tumor microenvironment. These results underscore the importance of DNMT-mediated DNA methylation in adipogenesis and breast cancer.https://cst.kipmi.or.id/journal/article/view/1678dna methylation; dna methyltransferase; adipogenesis; obesity-induced breast cancer; epigenetics; me3; tumor microenvironment; stat1; cpg islands; molecular subtypes. |
| spellingShingle | Sandeep Mallya Sharath Mohan Bhat Sachin Naie Sumati Kulkarni Manjunath B Joshi Shama Prasada Kabekkodu Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer Communications in Science and Technology dna methylation; dna methyltransferase; adipogenesis; obesity-induced breast cancer; epigenetics; me3; tumor microenvironment; stat1; cpg islands; molecular subtypes. |
| title | Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer |
| title_full | Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer |
| title_fullStr | Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer |
| title_full_unstemmed | Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer |
| title_short | Investigation of DNMT-mediated DNA methylation and its role in adipogenesis and breast cancer |
| title_sort | investigation of dnmt mediated dna methylation and its role in adipogenesis and breast cancer |
| topic | dna methylation; dna methyltransferase; adipogenesis; obesity-induced breast cancer; epigenetics; me3; tumor microenvironment; stat1; cpg islands; molecular subtypes. |
| url | https://cst.kipmi.or.id/journal/article/view/1678 |
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