Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail
This study investigated the effects of dietary glutamine (Gln) supplementation on the performance, carcass characteristics, blood biochemistry, intestinal morphology, and gut microbiota of laying quail over a 12-week period, with analyses conducted after 6 and 12 weeks of supplementation. Seven-week...
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001166 |
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| author | Ewa Tomaszewska Kamil Drabik Kornel Kasperek Piotr Dobrowolski Monika Hułas-Stasiak Renata Pyz-Łukasik Waldemar Paszkiewicz Monika Ziomek Janine Donaldson Katarzyna Dobrowolska Artur Burmaczuk Justyna Batkowska Marcin B. Arciszewski Siemowit Muszyński |
| author_facet | Ewa Tomaszewska Kamil Drabik Kornel Kasperek Piotr Dobrowolski Monika Hułas-Stasiak Renata Pyz-Łukasik Waldemar Paszkiewicz Monika Ziomek Janine Donaldson Katarzyna Dobrowolska Artur Burmaczuk Justyna Batkowska Marcin B. Arciszewski Siemowit Muszyński |
| author_sort | Ewa Tomaszewska |
| collection | DOAJ |
| description | This study investigated the effects of dietary glutamine (Gln) supplementation on the performance, carcass characteristics, blood biochemistry, intestinal morphology, and gut microbiota of laying quail over a 12-week period, with analyses conducted after 6 and 12 weeks of supplementation. Seven-week-old dual-purpose female quails at the onset of laying were assigned to diets containing 0% (control), 0.5%, 1%, or 1.5% Gln. Performance metrics included laying rate, daily feed intake, and feed efficiency. Supplementation with 0.5% Gln significantly enhanced the laying rate after 12 weeks; daily feed intake and feed efficiency varied over time but were not consistently affected by Gln. Breast muscle weight was highest in quail supplemented with 1.5% Gln after 6 weeks. Blood serum analyses showed that total protein and albumin concentrations were highest in quail receiving 0% and 1% Gln after 12 weeks, while supplementation with 0.5% Gln decreased triacylglycerol levels after 12 weeks. Glucose and creatinine levels varied with Gln supplementation and between the two time points; enzyme activities (alanine transaminase, alkaline phosphatase, and gamma-glutamyl transpeptidase) were affected by both Gln dose and length of supplementation. Intestinal morphology was significantly influenced by Gln dose and supplementation period. The longest villi were observed in quail supplemented with 1% Gln after 6 weeks, decreasing by 12 weeks. The widest villi were recorded in quail receiving 1.5% Gln after 6 weeks, with villus width decreasing over time. Crypt depth varied significantly, with the deepest crypts observed at 6 weeks in quail supplemented with 1% and 1.5% Gln, becoming shallower after 12 weeks. Microbial analysis of digesta samples revealed Lactobacillaceae as the predominant bacterial family, followed by Enterobacteriaceae and genera including Streptococcus and Staphylococcus. Overall, dietary Gln supplementation influenced performance, carcass characteristics, blood biochemical parameters, intestinal morphology, and gut microbiota composition in laying quail, with effects varying between 6 and 12 weeks of supplementation. These findings suggest that Gln supplementation has the potential to enhance quail performance and health over time. |
| format | Article |
| id | doaj-art-9f46f870b21a4fec8a5f11d4a41aa716 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-9f46f870b21a4fec8a5f11d4a41aa7162025-02-10T04:33:28ZengElsevierPoultry Science0032-57912025-03-011043104879Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quailEwa Tomaszewska0Kamil Drabik1Kornel Kasperek2Piotr Dobrowolski3Monika Hułas-Stasiak4Renata Pyz-Łukasik5Waldemar Paszkiewicz6Monika Ziomek7Janine Donaldson8Katarzyna Dobrowolska9Artur Burmaczuk10Justyna Batkowska11Marcin B. Arciszewski12Siemowit Muszyński13Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 12 Akademicka St., Lublin 20-950, Poland; Corresponding author.Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Lublin 20-950, PolandInstitute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Lublin 20-950, PolandDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin 20-033, PolandDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin 20-033, PolandDepartment of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-033, PolandDepartment of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-033, PolandDepartment of Food Hygiene of Animal Origin, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-033, PolandSchool of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South AfricaDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Lublin 20-033, PolandDepartment of Pharmacology, Toxicology and Environmental Protection, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-950, PolandInstitute of Biological Basis of Animal Production, University of Life Sciences in Lublin, Lublin 20-950, PolandDepartment of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-950, PolandDepartment of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, Lublin 20-950, PolandThis study investigated the effects of dietary glutamine (Gln) supplementation on the performance, carcass characteristics, blood biochemistry, intestinal morphology, and gut microbiota of laying quail over a 12-week period, with analyses conducted after 6 and 12 weeks of supplementation. Seven-week-old dual-purpose female quails at the onset of laying were assigned to diets containing 0% (control), 0.5%, 1%, or 1.5% Gln. Performance metrics included laying rate, daily feed intake, and feed efficiency. Supplementation with 0.5% Gln significantly enhanced the laying rate after 12 weeks; daily feed intake and feed efficiency varied over time but were not consistently affected by Gln. Breast muscle weight was highest in quail supplemented with 1.5% Gln after 6 weeks. Blood serum analyses showed that total protein and albumin concentrations were highest in quail receiving 0% and 1% Gln after 12 weeks, while supplementation with 0.5% Gln decreased triacylglycerol levels after 12 weeks. Glucose and creatinine levels varied with Gln supplementation and between the two time points; enzyme activities (alanine transaminase, alkaline phosphatase, and gamma-glutamyl transpeptidase) were affected by both Gln dose and length of supplementation. Intestinal morphology was significantly influenced by Gln dose and supplementation period. The longest villi were observed in quail supplemented with 1% Gln after 6 weeks, decreasing by 12 weeks. The widest villi were recorded in quail receiving 1.5% Gln after 6 weeks, with villus width decreasing over time. Crypt depth varied significantly, with the deepest crypts observed at 6 weeks in quail supplemented with 1% and 1.5% Gln, becoming shallower after 12 weeks. Microbial analysis of digesta samples revealed Lactobacillaceae as the predominant bacterial family, followed by Enterobacteriaceae and genera including Streptococcus and Staphylococcus. Overall, dietary Gln supplementation influenced performance, carcass characteristics, blood biochemical parameters, intestinal morphology, and gut microbiota composition in laying quail, with effects varying between 6 and 12 weeks of supplementation. These findings suggest that Gln supplementation has the potential to enhance quail performance and health over time.http://www.sciencedirect.com/science/article/pii/S0032579125001166GlutamineJapanese quailIntestine |
| spellingShingle | Ewa Tomaszewska Kamil Drabik Kornel Kasperek Piotr Dobrowolski Monika Hułas-Stasiak Renata Pyz-Łukasik Waldemar Paszkiewicz Monika Ziomek Janine Donaldson Katarzyna Dobrowolska Artur Burmaczuk Justyna Batkowska Marcin B. Arciszewski Siemowit Muszyński Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail Poultry Science Glutamine Japanese quail Intestine |
| title | Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail |
| title_full | Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail |
| title_fullStr | Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail |
| title_full_unstemmed | Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail |
| title_short | Evaluating the effects of dietary glutamine on performance, carcass traits, blood biochemistry, and intestine morphology in laying quail |
| title_sort | evaluating the effects of dietary glutamine on performance carcass traits blood biochemistry and intestine morphology in laying quail |
| topic | Glutamine Japanese quail Intestine |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001166 |
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