Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency
To investigate effects of reduced Leishman staining time on cell morphology, 3 thin blood films were made for 60 subjects. First group were flooded with Leishman stain for 3 minutes and further stained with diluted stain for 12 minutes while second group were stained for 6 minutes after initial floo...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2014-08-01
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| Series: | Mediterranean Journal of Hematology and Infectious Diseases |
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| Online Access: | https://mjhid.org/index.php/mjhid/article/view/1727 |
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| author | MUSA ABIDEMI MUHIBI RASIDAT OLUBUNMI HASSAN TAWAKALTU O ZAKARIYAHU BUSRA A TIJANI ABDUL WASIU O HASSAN OLUWASEUN S AKANDE MULIKAT O MUHIBI |
| author_facet | MUSA ABIDEMI MUHIBI RASIDAT OLUBUNMI HASSAN TAWAKALTU O ZAKARIYAHU BUSRA A TIJANI ABDUL WASIU O HASSAN OLUWASEUN S AKANDE MULIKAT O MUHIBI |
| author_sort | MUSA ABIDEMI MUHIBI |
| collection | DOAJ |
| description | To investigate effects of reduced Leishman staining time on cell morphology, 3 thin blood films were made for 60 subjects. First group were flooded with Leishman stain for 3 minutes and further stained with diluted stain for 12 minutes while second group were stained for 6 minutes after initial flooding for 3 minutes. Third group were exposed to undiluted stain for 3 minutes only. The differential WBC values were determined and presence of malaria parasites and sickle cells were noted. Mean ± SEM of the results compared using SPSS software showed no significant difference in all the cells (P>0.05) except in monocytes values between ‘3minutes’ and ‘15 minutes’ procedures (P<0.05). Presence of sickle cells and malaria parasites were recorded in 2 slides (3.3%) and 5 slides (8.3%) respectively across board. It could be concluded that 3 minutes and 9 minutes are enough to stain thin blood films. |
| format | Article |
| id | doaj-art-9f3f99f7afed420caaac5afcc1e90383 |
| institution | Kabale University |
| issn | 2035-3006 |
| language | English |
| publishDate | 2014-08-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Mediterranean Journal of Hematology and Infectious Diseases |
| spelling | doaj-art-9f3f99f7afed420caaac5afcc1e903832025-01-02T00:36:51ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062014-08-011Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergencyMUSA ABIDEMI MUHIBI0RASIDAT OLUBUNMI HASSANTAWAKALTU O ZAKARIYAHUBUSRA A TIJANIABDUL WASIU O HASSANOLUWASEUN S AKANDEMULIKAT O MUHIBILAUTECH TEACHING HOSPITAL, OSOGBO, OSUN STATE, NIGERIATo investigate effects of reduced Leishman staining time on cell morphology, 3 thin blood films were made for 60 subjects. First group were flooded with Leishman stain for 3 minutes and further stained with diluted stain for 12 minutes while second group were stained for 6 minutes after initial flooding for 3 minutes. Third group were exposed to undiluted stain for 3 minutes only. The differential WBC values were determined and presence of malaria parasites and sickle cells were noted. Mean ± SEM of the results compared using SPSS software showed no significant difference in all the cells (P>0.05) except in monocytes values between ‘3minutes’ and ‘15 minutes’ procedures (P<0.05). Presence of sickle cells and malaria parasites were recorded in 2 slides (3.3%) and 5 slides (8.3%) respectively across board. It could be concluded that 3 minutes and 9 minutes are enough to stain thin blood films.https://mjhid.org/index.php/mjhid/article/view/1727Leishman stainSOPBlood filmEmergency |
| spellingShingle | MUSA ABIDEMI MUHIBI RASIDAT OLUBUNMI HASSAN TAWAKALTU O ZAKARIYAHU BUSRA A TIJANI ABDUL WASIU O HASSAN OLUWASEUN S AKANDE MULIKAT O MUHIBI Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency Mediterranean Journal of Hematology and Infectious Diseases Leishman stain SOP Blood film Emergency |
| title | Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency |
| title_full | Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency |
| title_fullStr | Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency |
| title_full_unstemmed | Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency |
| title_short | Shortened staining procedures of thin blood films with leishman stain; a suitable alternative under emergency |
| title_sort | shortened staining procedures of thin blood films with leishman stain a suitable alternative under emergency |
| topic | Leishman stain SOP Blood film Emergency |
| url | https://mjhid.org/index.php/mjhid/article/view/1727 |
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