The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine

Background. The purpose of this study was to evaluate the combination of an enamel matrix derivative (EMD) and an osteoconductive bone ceramic (BC) in improving bone regeneration. Materials and Methods. Four cylindrical cavities (6×6mm) were prepared bilaterally in the mandible in three dogs. The de...

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Main Authors: Reza Birang, Mohammad Shah Abouei, Sayed Mohammad Razavi, Peyaman Zia, Ahmad Soolari
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/196791
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author Reza Birang
Mohammad Shah Abouei
Sayed Mohammad Razavi
Peyaman Zia
Ahmad Soolari
author_facet Reza Birang
Mohammad Shah Abouei
Sayed Mohammad Razavi
Peyaman Zia
Ahmad Soolari
author_sort Reza Birang
collection DOAJ
description Background. The purpose of this study was to evaluate the combination of an enamel matrix derivative (EMD) and an osteoconductive bone ceramic (BC) in improving bone regeneration. Materials and Methods. Four cylindrical cavities (6×6mm) were prepared bilaterally in the mandible in three dogs. The defects were randomly assigned to four different treatments—filled with EMD/BC and covered with a nonresorbable membrane, filled with EMD/BC without membrane, membrane coverage only, or control (left untreated)—and healed for 2, 4, or 6 weeks. Harvested specimens were prepared for histologic, histomorphometric, and immunohistochemical analyses. Results. Sites treated with EMD/BC with or without membrane showed more total bone formation and lamellar bone formation than membrane-only and control defects. There were no statistically significant differences in total bone formation between EMD/BC with or without membrane. Conclusion. EMD with BC might improve bone formation in osseous defects more than membrane coverage alone; the use of a membrane had no significant additive effect on total bone formation.
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institution Kabale University
issn 1537-744X
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publishDate 2012-01-01
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series The Scientific World Journal
spelling doaj-art-9f3ce2e67d6c44dca7868bdc636c43a02025-08-20T03:38:30ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/196791196791The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the CanineReza Birang0Mohammad Shah Abouei1Sayed Mohammad Razavi2Peyaman Zia3Ahmad Soolari4Department of Periodontics, School of Dentistry and Torabinejad Research Centre, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Periodontics, School of Dentistry and Torabinejad Research Centre, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pathology, School of Dentistry and Torabinejad Research Centre, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Periodontics, School of Dentistry and Torabinejad Research Centre, Isfahan University of Medical Sciences, Isfahan, IranDiplomate of American Board of Periodontology, Private Practice in Periodontics, Silver Spring and Potomac, 11616 Toulone Dr., Potomac, Maryland, MD 20854, USABackground. The purpose of this study was to evaluate the combination of an enamel matrix derivative (EMD) and an osteoconductive bone ceramic (BC) in improving bone regeneration. Materials and Methods. Four cylindrical cavities (6×6mm) were prepared bilaterally in the mandible in three dogs. The defects were randomly assigned to four different treatments—filled with EMD/BC and covered with a nonresorbable membrane, filled with EMD/BC without membrane, membrane coverage only, or control (left untreated)—and healed for 2, 4, or 6 weeks. Harvested specimens were prepared for histologic, histomorphometric, and immunohistochemical analyses. Results. Sites treated with EMD/BC with or without membrane showed more total bone formation and lamellar bone formation than membrane-only and control defects. There were no statistically significant differences in total bone formation between EMD/BC with or without membrane. Conclusion. EMD with BC might improve bone formation in osseous defects more than membrane coverage alone; the use of a membrane had no significant additive effect on total bone formation.http://dx.doi.org/10.1100/2012/196791
spellingShingle Reza Birang
Mohammad Shah Abouei
Sayed Mohammad Razavi
Peyaman Zia
Ahmad Soolari
The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
The Scientific World Journal
title The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
title_full The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
title_fullStr The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
title_full_unstemmed The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
title_short The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
title_sort effect of an enamel matrix derivative emdogain combined with bone ceramic on bone formation in mandibular defects a histomorphometric and immunohistochemical study in the canine
url http://dx.doi.org/10.1100/2012/196791
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