In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study
Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies includin...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/8309256 |
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| author | Manuel Mata Lara Milian Maria Oliver Javier Zurriaga Maria Sancho-Tello Jose Javier Martin de Llano Carmen Carda |
| author_facet | Manuel Mata Lara Milian Maria Oliver Javier Zurriaga Maria Sancho-Tello Jose Javier Martin de Llano Carmen Carda |
| author_sort | Manuel Mata |
| collection | DOAJ |
| description | Osteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). The effectiveness of ACI has been shown in vitro and in vivo, but the use of other cell types, including bone marrow and adipose-derived mesenchymal stem cells, is necessary because of the poor proliferation rate of isolated articular chondrocytes. In this investigation, we assessed the chondrogenic ability of human dental pulp stem cells (hDPSCs) to regenerate cartilage in vitro and in vivo. hDPSCs and primary isolated rabbit chondrocytes were cultured in chondrogenic culture medium and found to express collagen II and aggrecan. Both cell types were cultured in 3% alginate hydrogels and implanted in a rabbit model of cartilage damage. Three months after surgery, significant cartilage regeneration was observed, particularly in the animals implanted with hDPSCs. Although the results presented here are preliminary, they suggest that hDPSCs may be useful for regeneration of articular cartilage. |
| format | Article |
| id | doaj-art-9f38af542dc44d68b5aef6407f5c63dc |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-9f38af542dc44d68b5aef6407f5c63dc2025-08-20T03:38:27ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/83092568309256In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary StudyManuel Mata0Lara Milian1Maria Oliver2Javier Zurriaga3Maria Sancho-Tello4Jose Javier Martin de Llano5Carmen Carda6Department of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainDepartment of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainDepartment of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainHospital Clinico Universitario de Valencia, Blasco Ibañez Avenue 17, 46010 Valencia, SpainDepartment of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainDepartment of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainDepartment of Pathology, Faculty of Medicine and Odontology, University of Valencia, Blasco Ibáñez Avenue 15, 46010 Valencia, SpainOsteoarthritis is an inflammatory disease in which all joint-related elements, articular cartilage in particular, are affected. The poor regeneration capacity of this tissue together with the lack of pharmacological treatment has led to the development of regenerative medicine methodologies including microfracture and autologous chondrocyte implantation (ACI). The effectiveness of ACI has been shown in vitro and in vivo, but the use of other cell types, including bone marrow and adipose-derived mesenchymal stem cells, is necessary because of the poor proliferation rate of isolated articular chondrocytes. In this investigation, we assessed the chondrogenic ability of human dental pulp stem cells (hDPSCs) to regenerate cartilage in vitro and in vivo. hDPSCs and primary isolated rabbit chondrocytes were cultured in chondrogenic culture medium and found to express collagen II and aggrecan. Both cell types were cultured in 3% alginate hydrogels and implanted in a rabbit model of cartilage damage. Three months after surgery, significant cartilage regeneration was observed, particularly in the animals implanted with hDPSCs. Although the results presented here are preliminary, they suggest that hDPSCs may be useful for regeneration of articular cartilage.http://dx.doi.org/10.1155/2017/8309256 |
| spellingShingle | Manuel Mata Lara Milian Maria Oliver Javier Zurriaga Maria Sancho-Tello Jose Javier Martin de Llano Carmen Carda In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study Stem Cells International |
| title | In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study |
| title_full | In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study |
| title_fullStr | In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study |
| title_full_unstemmed | In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study |
| title_short | In Vivo Articular Cartilage Regeneration Using Human Dental Pulp Stem Cells Cultured in an Alginate Scaffold: A Preliminary Study |
| title_sort | in vivo articular cartilage regeneration using human dental pulp stem cells cultured in an alginate scaffold a preliminary study |
| url | http://dx.doi.org/10.1155/2017/8309256 |
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