Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs
Hereditary factors contribute significantly to the development of schizophrenia. However, despite many years of research, the genetic architecture and mechanisms of the participation of genetic factors in the development of schizophrenia are not well understood. Genome-wide analyzes of genetic assoc...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Federal State Budget Scientific Institution National Medical Research Center for Psychiatry and Neurology n.a. V.M. Bekhterev Ministry of Health of the Russian Federation
2019-12-01
|
| Series: | Обозрение психиатрии и медицинской психологии имени В.М. Бехтерева |
| Subjects: | |
| Online Access: | https://www.bekhterevreview.com/jour/article/view/324 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849693578854400000 |
|---|---|
| author | D. A. Abashkin A. O. Kurishev S. V. Smirnova D. S. Karpov V. E. Golimbet |
| author_facet | D. A. Abashkin A. O. Kurishev S. V. Smirnova D. S. Karpov V. E. Golimbet |
| author_sort | D. A. Abashkin |
| collection | DOAJ |
| description | Hereditary factors contribute significantly to the development of schizophrenia. However, despite many years of research, the genetic architecture and mechanisms of the participation of genetic factors in the development of schizophrenia are not well understood. Genome-wide analyzes of genetic associations in various non-coding regions of the genome, including gene enhancers, revealed many loci associated with an increased risk of schizophrenia. In the course of the analysis of the spatial structure of the genome, we revealed the interaction of these enhancers with the promoter regions of genes involved in the metabolism of neurons. To study in more detail the functions of these genes and the participation of enhancers in their regulation, we obtained plasmid and lentiviral constructs of a functionally active transcription repressor based on the CRISPR / SpyCas9 system, as well as the endonuclease system. The use of these constructs in studies of the functions of enhancers and genes associated with the metabolism and regulation of gene expression in neurons is discussed. |
| format | Article |
| id | doaj-art-9f2d85f8b52a478ead0ee1b2ef410bde |
| institution | DOAJ |
| issn | 2313-7053 2713-055X |
| language | Russian |
| publishDate | 2019-12-01 |
| publisher | Federal State Budget Scientific Institution National Medical Research Center for Psychiatry and Neurology n.a. V.M. Bekhterev Ministry of Health of the Russian Federation |
| record_format | Article |
| series | Обозрение психиатрии и медицинской психологии имени В.М. Бехтерева |
| spelling | doaj-art-9f2d85f8b52a478ead0ee1b2ef410bde2025-08-20T03:20:22ZrusFederal State Budget Scientific Institution National Medical Research Center for Psychiatry and Neurology n.a. V.M. Bekhterev Ministry of Health of the Russian FederationОбозрение психиатрии и медицинской психологии имени В.М. Бехтерева2313-70532713-055X2019-12-0104-110310510.31363/2313-7053-2019-4-1-103-105303Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructsD. A. Abashkin0A. O. Kurishev1S. V. Smirnova2D. S. Karpov3V. E. Golimbet4Mental Health Research CenterMental Health Research CenterMental Health Research CenterMental Health Research CenterMental Health Research CenterHereditary factors contribute significantly to the development of schizophrenia. However, despite many years of research, the genetic architecture and mechanisms of the participation of genetic factors in the development of schizophrenia are not well understood. Genome-wide analyzes of genetic associations in various non-coding regions of the genome, including gene enhancers, revealed many loci associated with an increased risk of schizophrenia. In the course of the analysis of the spatial structure of the genome, we revealed the interaction of these enhancers with the promoter regions of genes involved in the metabolism of neurons. To study in more detail the functions of these genes and the participation of enhancers in their regulation, we obtained plasmid and lentiviral constructs of a functionally active transcription repressor based on the CRISPR / SpyCas9 system, as well as the endonuclease system. The use of these constructs in studies of the functions of enhancers and genes associated with the metabolism and regulation of gene expression in neurons is discussed.https://www.bekhterevreview.com/jour/article/view/324schizophrenia, enhancers, crispr / spycas9 |
| spellingShingle | D. A. Abashkin A. O. Kurishev S. V. Smirnova D. S. Karpov V. E. Golimbet Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs Обозрение психиатрии и медицинской психологии имени В.М. Бехтерева schizophrenia, enhancers, crispr / spycas9 |
| title | Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs |
| title_full | Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs |
| title_fullStr | Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs |
| title_full_unstemmed | Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs |
| title_short | Epigenome editing of the potential enhancers of the schizophrenia risk genes: approaches for optimization of the genetic constructs |
| title_sort | epigenome editing of the potential enhancers of the schizophrenia risk genes approaches for optimization of the genetic constructs |
| topic | schizophrenia, enhancers, crispr / spycas9 |
| url | https://www.bekhterevreview.com/jour/article/view/324 |
| work_keys_str_mv | AT daabashkin epigenomeeditingofthepotentialenhancersoftheschizophreniariskgenesapproachesforoptimizationofthegeneticconstructs AT aokurishev epigenomeeditingofthepotentialenhancersoftheschizophreniariskgenesapproachesforoptimizationofthegeneticconstructs AT svsmirnova epigenomeeditingofthepotentialenhancersoftheschizophreniariskgenesapproachesforoptimizationofthegeneticconstructs AT dskarpov epigenomeeditingofthepotentialenhancersoftheschizophreniariskgenesapproachesforoptimizationofthegeneticconstructs AT vegolimbet epigenomeeditingofthepotentialenhancersoftheschizophreniariskgenesapproachesforoptimizationofthegeneticconstructs |