Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress
The aggravation of soil salinization has become one of the major factors that threaten crop growth and yield. Rhizobia, as an important biological nitrogen-fixing microorganism, can establish symbiotic relationships with legumes to improve their nitrogen-fixing ability and stress tolerance. Trehalos...
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MDPI AG
2025-04-01
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| Series: | Agronomy |
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| author | Jialin Liu Dong Wang Ruiqi Tong Shengyue Ye Yanhao Zhao Jiangwen Wu Yi Gan |
| author_facet | Jialin Liu Dong Wang Ruiqi Tong Shengyue Ye Yanhao Zhao Jiangwen Wu Yi Gan |
| author_sort | Jialin Liu |
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| description | The aggravation of soil salinization has become one of the major factors that threaten crop growth and yield. Rhizobia, as an important biological nitrogen-fixing microorganism, can establish symbiotic relationships with legumes to improve their nitrogen-fixing ability and stress tolerance. Trehalose, a non-reducing disaccharide that is widely found in bacteria, fungi, and plants, can protect cellular structures and maintain the viability of cells under stress conditions. However, it remains to be determined whether the endogenous trehalose level in rhizobia could affect its stress tolerance and nitrogen-fixing capabilities. In this study, we constructed four engineered rhizobial strains to examine the effects of the overexpression and knockout of the trehalose synthesis genes <i>otsA</i>/<i>otsB</i> in the rhizobium strain <i>CCBAU25338</i> on its salt tolerance and nitrogen-fixing capacity. The results indicated that the overexpression of <i>otsA</i>, rather than the <i>otsB</i> gene, significantly enhanced both the stress tolerance and nitrogen-fixing abilities of the strains. Furthermore, the inoculation of <i>otsA</i>-overexpressing recombinant cells leads to greater agronomic traits in the host plant’s peanuts under salinity conditions. We hope our findings may serve as valuable references for the future development of efficient and superior engineered rhizobial strains for peanut cultivation. |
| format | Article |
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| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-9f0fbdf8cb0c4bb892515ec58ba1fe782025-08-20T03:14:23ZengMDPI AGAgronomy2073-43952025-04-0115497410.3390/agronomy15040974Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity StressJialin Liu0Dong Wang1Ruiqi Tong2Shengyue Ye3Yanhao Zhao4Jiangwen Wu5Yi Gan6College of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, ChinaTonglu County Agricultural Industrialization Development Service Center, Hangzhou 311300, ChinaTonglu County Agricultural Technology Extension Center, Hangzhou 311500, ChinaShitang Town People’s Government of Yunhe County, Lishui 323604, ChinaCollege of Advanced Agricultural Sciences, Zhejiang A & F University, Hangzhou 311300, ChinaThe aggravation of soil salinization has become one of the major factors that threaten crop growth and yield. Rhizobia, as an important biological nitrogen-fixing microorganism, can establish symbiotic relationships with legumes to improve their nitrogen-fixing ability and stress tolerance. Trehalose, a non-reducing disaccharide that is widely found in bacteria, fungi, and plants, can protect cellular structures and maintain the viability of cells under stress conditions. However, it remains to be determined whether the endogenous trehalose level in rhizobia could affect its stress tolerance and nitrogen-fixing capabilities. In this study, we constructed four engineered rhizobial strains to examine the effects of the overexpression and knockout of the trehalose synthesis genes <i>otsA</i>/<i>otsB</i> in the rhizobium strain <i>CCBAU25338</i> on its salt tolerance and nitrogen-fixing capacity. The results indicated that the overexpression of <i>otsA</i>, rather than the <i>otsB</i> gene, significantly enhanced both the stress tolerance and nitrogen-fixing abilities of the strains. Furthermore, the inoculation of <i>otsA</i>-overexpressing recombinant cells leads to greater agronomic traits in the host plant’s peanuts under salinity conditions. We hope our findings may serve as valuable references for the future development of efficient and superior engineered rhizobial strains for peanut cultivation.https://www.mdpi.com/2073-4395/15/4/974trehaloserhizobiumpeanuts<i>otsA</i><i>otsB</i>salt stress |
| spellingShingle | Jialin Liu Dong Wang Ruiqi Tong Shengyue Ye Yanhao Zhao Jiangwen Wu Yi Gan Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress Agronomy trehalose rhizobium peanuts <i>otsA</i> <i>otsB</i> salt stress |
| title | Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress |
| title_full | Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress |
| title_fullStr | Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress |
| title_full_unstemmed | Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress |
| title_short | Engineered Rhizobia with Trehalose-Producing Genes Enhance Peanut Growth Under Salinity Stress |
| title_sort | engineered rhizobia with trehalose producing genes enhance peanut growth under salinity stress |
| topic | trehalose rhizobium peanuts <i>otsA</i> <i>otsB</i> salt stress |
| url | https://www.mdpi.com/2073-4395/15/4/974 |
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