Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition
Personalized medicine for breast cancer (BrC) requires predictive biomarkers to select the optimal therapeutic option for each individual patient. Personalization of chemotherapy or immunotherapy responses is particularly challenging, as molecular markers do not appear to be sufficiently predictive...
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| Format: | Article |
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Elsevier
2025-10-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558625001009 |
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| author | Zofia M. Komar Mieke Bavelaar Ellen Kageler Nicole S. Verkaik Mandy M. van Rosmalen Carolien H.M. van Deurzen Michael A. den Bakker Roland Kanaar Adriaan B Houtsmuller Thierry P.P. van den Bosch Agnes Jager Dik C. van Gent |
| author_facet | Zofia M. Komar Mieke Bavelaar Ellen Kageler Nicole S. Verkaik Mandy M. van Rosmalen Carolien H.M. van Deurzen Michael A. den Bakker Roland Kanaar Adriaan B Houtsmuller Thierry P.P. van den Bosch Agnes Jager Dik C. van Gent |
| author_sort | Zofia M. Komar |
| collection | DOAJ |
| description | Personalized medicine for breast cancer (BrC) requires predictive biomarkers to select the optimal therapeutic option for each individual patient. Personalization of chemotherapy or immunotherapy responses is particularly challenging, as molecular markers do not appear to be sufficiently predictive for therapy response. Functional assays for therapy selection may be the solution for this dilemma. An interesting approach is ex vivo cultures of precision cut tumor slices, such as the MOtion-based Tissue EX vivo (MOTEX) method that we described previously. This culture method has the advantage that it carries all cell types in the tumor, including various immune cell populations. We here show, that macrophages, B-cells and T-cell populations are maintained in the MOTEX culture for several days without apparent loss of viability. Even treatment with the microtubule poison paclitaxel did not reduce immune cell abundance or viability significantly. Anthracycline-based chemotherapy, however, did affect immune cell composition, as expected based on its cytotoxic properties. Therefore, we conclude that MOTEX culture of BrC tissue slices can be used to investigate effect of treatments that involve the immune system. This opens perspectives to develop predictive assays for immune checkpoint inhibitor treatment and other therapeutic interventions that require immune components in the assay system. |
| format | Article |
| id | doaj-art-9ef04bc0c7614f5094addf89fc134d22 |
| institution | Kabale University |
| issn | 1476-5586 |
| language | English |
| publishDate | 2025-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj-art-9ef04bc0c7614f5094addf89fc134d222025-08-22T04:55:53ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55862025-10-016810122110.1016/j.neo.2025.101221Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment compositionZofia M. Komar0Mieke Bavelaar1Ellen Kageler2Nicole S. Verkaik3Mandy M. van Rosmalen4Carolien H.M. van Deurzen5Michael A. den Bakker6Roland Kanaar7Adriaan B Houtsmuller8Thierry P.P. van den Bosch9Agnes Jager10Dik C. van Gent11Department of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Pathology, Erasmus MC, Rotterdam, the NetherlandsDepartment of Pathology, Maasstad Ziekenhuis, Rotterdam, the NetherlandsDepartment of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The Netherlands; Oncode Institute, Erasmus MC, Rotterdam, The NetherlandsErasmus Optical Imaging Center and Department of Pathology, Erasmus MC, Rotterdam, the NetherlandsDepartment of Pathology, Erasmus MC, Rotterdam, the NetherlandsDepartment of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, The NetherlandsDepartment of Molecular Genetics, Erasmus MC Cancer Institute, Rotterdam, The Netherlands; Oncode Institute, Erasmus MC, Rotterdam, The NetherlandsPersonalized medicine for breast cancer (BrC) requires predictive biomarkers to select the optimal therapeutic option for each individual patient. Personalization of chemotherapy or immunotherapy responses is particularly challenging, as molecular markers do not appear to be sufficiently predictive for therapy response. Functional assays for therapy selection may be the solution for this dilemma. An interesting approach is ex vivo cultures of precision cut tumor slices, such as the MOtion-based Tissue EX vivo (MOTEX) method that we described previously. This culture method has the advantage that it carries all cell types in the tumor, including various immune cell populations. We here show, that macrophages, B-cells and T-cell populations are maintained in the MOTEX culture for several days without apparent loss of viability. Even treatment with the microtubule poison paclitaxel did not reduce immune cell abundance or viability significantly. Anthracycline-based chemotherapy, however, did affect immune cell composition, as expected based on its cytotoxic properties. Therefore, we conclude that MOTEX culture of BrC tissue slices can be used to investigate effect of treatments that involve the immune system. This opens perspectives to develop predictive assays for immune checkpoint inhibitor treatment and other therapeutic interventions that require immune components in the assay system.http://www.sciencedirect.com/science/article/pii/S1476558625001009Tumor microenvironmentBreast cancerTreatment predictionTumor infiltrating lymphocytes (TILs) |
| spellingShingle | Zofia M. Komar Mieke Bavelaar Ellen Kageler Nicole S. Verkaik Mandy M. van Rosmalen Carolien H.M. van Deurzen Michael A. den Bakker Roland Kanaar Adriaan B Houtsmuller Thierry P.P. van den Bosch Agnes Jager Dik C. van Gent Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition Neoplasia: An International Journal for Oncology Research Tumor microenvironment Breast cancer Treatment prediction Tumor infiltrating lymphocytes (TILs) |
| title | Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition |
| title_full | Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition |
| title_fullStr | Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition |
| title_full_unstemmed | Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition |
| title_short | Motion based ex vivo (MOTEX) culture of breast tumor slices sustains microenvironment composition |
| title_sort | motion based ex vivo motex culture of breast tumor slices sustains microenvironment composition |
| topic | Tumor microenvironment Breast cancer Treatment prediction Tumor infiltrating lymphocytes (TILs) |
| url | http://www.sciencedirect.com/science/article/pii/S1476558625001009 |
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