Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food
The detection of Listeria monocytogenes in food samples using enrichment and PCR is described. The objectives were to determine whether the type of enrichment medium has the influence on the results obtained with PCR and to evaluate proposed method by analysing naturally contaminated food samples. C...
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| Format: | Article |
| Language: | English |
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University of Ljubljana Press (Založba Univerze v Ljubljani)
2005-05-01
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| Series: | Acta Agriculturae Slovenica |
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| Online Access: | https://journals.uni-lj.si/aas/article/view/15256 |
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| author | Barbara JERŠEK Tamara MAJSTOROVIĆ Nataša KLUN Sonja SMOLE MOŽINA |
| author_facet | Barbara JERŠEK Tamara MAJSTOROVIĆ Nataša KLUN Sonja SMOLE MOŽINA |
| author_sort | Barbara JERŠEK |
| collection | DOAJ |
| description | The detection of Listeria monocytogenes in food samples using enrichment and PCR is described. The objectives were to determine whether the type of enrichment medium has the influence on the results obtained with PCR and to evaluate proposed method by analysing naturally contaminated food samples. Comparative evaluation of different enrichment media for bacteria of the genus Listeria (half Fraser - HF, buffered peptone water - BPW, triptic soy yeast extract broth - TSBYE, universal preenrichment broth - UPB) showed that only the UPB enabled detection of L. monocytogenes in artificially contaminated food samples after 24h of incubation. The PCR-based method gave equal results as standard cultural method by analysis of naturally contaminated food samples. Described PCR-based procedure is comparable to some other PCR-based methods of L. monocytogenes detection in foods and shows promise as a rapid, routine method which requires 30-72 h, whereas cultural methods require 96-120 h. |
| format | Article |
| id | doaj-art-9eee1c16b40645c79d9ee815a5bf9941 |
| institution | DOAJ |
| issn | 1854-1941 |
| language | English |
| publishDate | 2005-05-01 |
| publisher | University of Ljubljana Press (Založba Univerze v Ljubljani) |
| record_format | Article |
| series | Acta Agriculturae Slovenica |
| spelling | doaj-art-9eee1c16b40645c79d9ee815a5bf99412025-08-20T03:02:26ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412005-05-01851152310.14720/aas.2005.85.1.1525621649Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in foodBarbara JERŠEK0Tamara MAJSTOROVIĆ1Nataša KLUN2Sonja SMOLE MOŽINA3Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1111 Ljubljana, SloveniaDepartment of Sanitary Microbiology, Institute of Public Health of the Republic of Slovenia, Grablovičeva 44, 1000 Ljubljana, SloveniaDepartment of Sanitary Microbiology, Institute of Public Health of the Republic of Slovenia, Grablovičeva 44, 1000 Ljubljana, SloveniaDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, 1111 Ljubljana, SloveniaThe detection of Listeria monocytogenes in food samples using enrichment and PCR is described. The objectives were to determine whether the type of enrichment medium has the influence on the results obtained with PCR and to evaluate proposed method by analysing naturally contaminated food samples. Comparative evaluation of different enrichment media for bacteria of the genus Listeria (half Fraser - HF, buffered peptone water - BPW, triptic soy yeast extract broth - TSBYE, universal preenrichment broth - UPB) showed that only the UPB enabled detection of L. monocytogenes in artificially contaminated food samples after 24h of incubation. The PCR-based method gave equal results as standard cultural method by analysis of naturally contaminated food samples. Described PCR-based procedure is comparable to some other PCR-based methods of L. monocytogenes detection in foods and shows promise as a rapid, routine method which requires 30-72 h, whereas cultural methods require 96-120 h.https://journals.uni-lj.si/aas/article/view/15256listeria monocytogenesuniversal preenrichment broth upbpcrdetectionfood hygiene |
| spellingShingle | Barbara JERŠEK Tamara MAJSTOROVIĆ Nataša KLUN Sonja SMOLE MOŽINA Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food Acta Agriculturae Slovenica listeria monocytogenes universal preenrichment broth upb pcr detection food hygiene |
| title | Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food |
| title_full | Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food |
| title_fullStr | Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food |
| title_full_unstemmed | Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food |
| title_short | Impact of enrichment medium on PCR-based detection of Listeria monocytogenes in food |
| title_sort | impact of enrichment medium on pcr based detection of listeria monocytogenes in food |
| topic | listeria monocytogenes universal preenrichment broth upb pcr detection food hygiene |
| url | https://journals.uni-lj.si/aas/article/view/15256 |
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