Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach
Summary: Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental des...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001376 |
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| author | Nadia Krarup Knudsen Christine Hjorth Andreassen Martin Blomberg Jensen Anne Jørgensen |
| author_facet | Nadia Krarup Knudsen Christine Hjorth Andreassen Martin Blomberg Jensen Anne Jørgensen |
| author_sort | Nadia Krarup Knudsen |
| collection | DOAJ |
| description | Summary: Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurements of reproductive hormones and paracrine factors secreted to the culture medium.For complete details on the use and execution of this protocol, please refer to Andreassen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-9ee67cb96bf145de95177748d2f02674 |
| institution | DOAJ |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-9ee67cb96bf145de95177748d2f026742025-08-20T03:05:24ZengElsevierSTAR Protocols2666-16672025-06-016210373110.1016/j.xpro.2025.103731Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approachNadia Krarup Knudsen0Christine Hjorth Andreassen1Martin Blomberg Jensen2Anne Jørgensen3Division of Translational Endocrinology, Department of Endocrinology and Internal Medicine, Copenhagen University Hospital - Herlev and Gentofte, Copenhagen, Denmark; Corresponding authorDivision of Translational Endocrinology, Department of Endocrinology and Internal Medicine, Copenhagen University Hospital - Herlev and Gentofte, Copenhagen, DenmarkDivision of Translational Endocrinology, Department of Endocrinology and Internal Medicine, Copenhagen University Hospital - Herlev and Gentofte, Copenhagen, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, DenmarkDivision of Translational Endocrinology, Department of Endocrinology and Internal Medicine, Copenhagen University Hospital - Herlev and Gentofte, Copenhagen, Denmark; Corresponding authorSummary: Testicular function relies on tightly regulated cell-cell interactions, including Sertoli-Leydig cell communication and Sertoli cells nurturing germ cells. Here, we present a 48-h ex vivo culture protocol for adult mouse testis tissue using a gel-based approach. We describe experimental design, dissection, and examination of treatment-induced outcomes, in particular, stimulatory effects on germ cells. We outline procedures for quantifying apoptosis and germ cell proliferation in the tissue and measurements of reproductive hormones and paracrine factors secreted to the culture medium.For complete details on the use and execution of this protocol, please refer to Andreassen et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725001376Cell biologyCell cultureCell isolationModel organisms |
| spellingShingle | Nadia Krarup Knudsen Christine Hjorth Andreassen Martin Blomberg Jensen Anne Jørgensen Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach STAR Protocols Cell biology Cell culture Cell isolation Model organisms |
| title | Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach |
| title_full | Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach |
| title_fullStr | Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach |
| title_full_unstemmed | Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach |
| title_short | Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach |
| title_sort | protocol for ex vivo culture of adult mouse testis tissue using a gel based approach |
| topic | Cell biology Cell culture Cell isolation Model organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725001376 |
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