Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities
This study was carried out to investigate the antioxidant properties of synbiotic product, Lactobacillus acidophilus supplemented with 2.5% royal jelly in skim milk and Bifidobacterium bifidum supplemented with 7.5% royal jelly in skim milk, using DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scaveng...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2014-09-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0015/pjfns-2013-0015.xml?format=INT |
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| author | Nabas Zaid Haddadin Malik S.Y. Haddadin Jamal Nazer Ibrahim K. |
| author_facet | Nabas Zaid Haddadin Malik S.Y. Haddadin Jamal Nazer Ibrahim K. |
| author_sort | Nabas Zaid |
| collection | DOAJ |
| description | This study was carried out to investigate the antioxidant properties of synbiotic product, Lactobacillus acidophilus supplemented with 2.5% royal jelly in skim milk and Bifidobacterium bifidum supplemented with 7.5% royal jelly in skim milk, using DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging assay, reducing power, total antioxidant in linoleic acid system and formation of diene-conjugation assay. Results showed that the synbiotic effect of royal jelly and probiotic bacteria provided substantial antioxidant activities. Milk samples fermented by B. bifi dum supplemented with 7.5% royal jelly and L. acidophilus supplemented with 2.5% royal jelly exhibited high scavenging activity with 96.8 and 93.3%, respectively, at a concentration of 500 μg/mL. IC50 values were estimated at 226.7 μg/mL for B. bifidum supplemented with 7.5% royal jelly and at 210.2 μg/ml for L. acidophilus supplemented with 2.5% royal jelly. On the other hand, L. acidophilus supplemented with 2.5% royal jelly and B. bifidum supplemented with 7.5% royal jelly exhibited significantly high reducing power at a concentration of 1000 μg/mL. The percentages of peroxide inhibition of L. acidophilus supplemented with 2.5% royal jelly and B. bifidum with 7.5% royal jelly were 52% and 42%, respectively. Significant inhibitions were found in the formation of conjugated diene at 66.9% and 65.8% for L. acidophilus with 2.5% royal jelly and B. bifidum with 7.5% royal jelly, respectively. These results were compared with standards BHT, ascorbic acid and Trolox. |
| format | Article |
| id | doaj-art-9ee512d7e4df4bcc8f5e47b615cb0b03 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2014-09-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-9ee512d7e4df4bcc8f5e47b615cb0b032025-02-02T05:22:56ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072014-09-0164317118010.2478/pjfns-2013-0015pjfns-2013-0015Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant ActivitiesNabas Zaid0Haddadin Malik S.Y.1Haddadin Jamal2Nazer Ibrahim K.3Department of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, JordanDepartment of Nutrition and Food Technology, Faculty of Agriculture, University of Jordan, Amman, JordanDepartment of Food and Nutrition Sciences, King Faisal University, Al Ahsa, Kingdom of Saudi ArabiaDepartment of Plant Protection, Faculty of Agriculture, University of Jordan, Amman, JordanThis study was carried out to investigate the antioxidant properties of synbiotic product, Lactobacillus acidophilus supplemented with 2.5% royal jelly in skim milk and Bifidobacterium bifidum supplemented with 7.5% royal jelly in skim milk, using DPPH (1,1-Diphenyl-2-picrylhydrazyl) radical scavenging assay, reducing power, total antioxidant in linoleic acid system and formation of diene-conjugation assay. Results showed that the synbiotic effect of royal jelly and probiotic bacteria provided substantial antioxidant activities. Milk samples fermented by B. bifi dum supplemented with 7.5% royal jelly and L. acidophilus supplemented with 2.5% royal jelly exhibited high scavenging activity with 96.8 and 93.3%, respectively, at a concentration of 500 μg/mL. IC50 values were estimated at 226.7 μg/mL for B. bifidum supplemented with 7.5% royal jelly and at 210.2 μg/ml for L. acidophilus supplemented with 2.5% royal jelly. On the other hand, L. acidophilus supplemented with 2.5% royal jelly and B. bifidum supplemented with 7.5% royal jelly exhibited significantly high reducing power at a concentration of 1000 μg/mL. The percentages of peroxide inhibition of L. acidophilus supplemented with 2.5% royal jelly and B. bifidum with 7.5% royal jelly were 52% and 42%, respectively. Significant inhibitions were found in the formation of conjugated diene at 66.9% and 65.8% for L. acidophilus with 2.5% royal jelly and B. bifidum with 7.5% royal jelly, respectively. These results were compared with standards BHT, ascorbic acid and Trolox.http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0015/pjfns-2013-0015.xml?format=INTsynbioticroyal jellyDPPHreducing powerlinoleic acid system |
| spellingShingle | Nabas Zaid Haddadin Malik S.Y. Haddadin Jamal Nazer Ibrahim K. Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities Polish Journal of Food and Nutrition Sciences synbiotic royal jelly DPPH reducing power linoleic acid system |
| title | Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities |
| title_full | Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities |
| title_fullStr | Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities |
| title_full_unstemmed | Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities |
| title_short | Chemical Composition of Royal Jelly and Effects of Synbiotic with Two Different Locally Isolated Probiotic Strains on Antioxidant Activities |
| title_sort | chemical composition of royal jelly and effects of synbiotic with two different locally isolated probiotic strains on antioxidant activities |
| topic | synbiotic royal jelly DPPH reducing power linoleic acid system |
| url | http://www.degruyter.com/view/j/pjfns.2014.64.issue-3/pjfns-2013-0015/pjfns-2013-0015.xml?format=INT |
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