Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury
Abstract Astrocytes have a beneficial role in tissue repair after central nervous system (CNS) injury. Although astrocyte proliferation is activated in response to injury, the intracellular mechanisms of astrocyte proliferation during acute phase of injury are not fully clarified. In this study, by...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Neuroinflammation |
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| Online Access: | https://doi.org/10.1186/s12974-025-03351-4 |
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| author | Lili Quan Akiko Uyeda Ichiro Manabe Rieko Muramatsu |
| author_facet | Lili Quan Akiko Uyeda Ichiro Manabe Rieko Muramatsu |
| author_sort | Lili Quan |
| collection | DOAJ |
| description | Abstract Astrocytes have a beneficial role in tissue repair after central nervous system (CNS) injury. Although astrocyte proliferation is activated in response to injury, the intracellular mechanisms of astrocyte proliferation during acute phase of injury are not fully clarified. In this study, by functionally screening the highly expressed genes in the pathological state of spinal astrocytes, heterogeneous nuclear ribonucleoprotein U (Hnrnpu) is identified as a potential endogenous molecule that regulates astrocyte proliferation and the following scar formation. Inhibition of Hnrnpu in astrocytes impairs the formation of astrocytic glial scar, motor function recovery, and neuronal regeneration after spinal cord injury (SCI) in mice. In human astrocytes, HNRNPU knockdown downregulates the genes related to the astrocyte functions in scar formation and neuronal regeneration. These findings uncover that modulation of endogenous astrocytic function would be a promising therapeutic avenue to restore neurological function after CNS injury. |
| format | Article |
| id | doaj-art-9ee1a97dc67c45708268114a51766d65 |
| institution | Kabale University |
| issn | 1742-2094 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj-art-9ee1a97dc67c45708268114a51766d652025-02-02T12:35:00ZengBMCJournal of Neuroinflammation1742-20942025-01-0122111610.1186/s12974-025-03351-4Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injuryLili Quan0Akiko Uyeda1Ichiro Manabe2Rieko Muramatsu3Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and PsychiatryDepartment of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and PsychiatryDepartment of Systems Medicine, Graduate School of Medicine, Chiba UniversityDepartment of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and PsychiatryAbstract Astrocytes have a beneficial role in tissue repair after central nervous system (CNS) injury. Although astrocyte proliferation is activated in response to injury, the intracellular mechanisms of astrocyte proliferation during acute phase of injury are not fully clarified. In this study, by functionally screening the highly expressed genes in the pathological state of spinal astrocytes, heterogeneous nuclear ribonucleoprotein U (Hnrnpu) is identified as a potential endogenous molecule that regulates astrocyte proliferation and the following scar formation. Inhibition of Hnrnpu in astrocytes impairs the formation of astrocytic glial scar, motor function recovery, and neuronal regeneration after spinal cord injury (SCI) in mice. In human astrocytes, HNRNPU knockdown downregulates the genes related to the astrocyte functions in scar formation and neuronal regeneration. These findings uncover that modulation of endogenous astrocytic function would be a promising therapeutic avenue to restore neurological function after CNS injury.https://doi.org/10.1186/s12974-025-03351-4AstrocytesSpinal cord injuryGlial scar formationRegeneration |
| spellingShingle | Lili Quan Akiko Uyeda Ichiro Manabe Rieko Muramatsu Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury Journal of Neuroinflammation Astrocytes Spinal cord injury Glial scar formation Regeneration |
| title | Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury |
| title_full | Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury |
| title_fullStr | Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury |
| title_full_unstemmed | Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury |
| title_short | Astrocytic heterogeneous nuclear ribonucleoprotein U is involved in scar formation after spinal cord injury |
| title_sort | astrocytic heterogeneous nuclear ribonucleoprotein u is involved in scar formation after spinal cord injury |
| topic | Astrocytes Spinal cord injury Glial scar formation Regeneration |
| url | https://doi.org/10.1186/s12974-025-03351-4 |
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