Development and Applications of Enhanced Green Fluorescent Protein Mutants
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon e...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1998-03-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/98243rr01 |
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| Summary: | The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold–100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells. |
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| ISSN: | 0736-6205 1940-9818 |