Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature

Abstract Background Spindle epithelial tumor with thymus-like differentiation or elements (SETTLE) is a rare and malignant biphasic tumor of the thyroid that has proven challenging to diagnose by cytology. Case presentation A 20-year-old-male presented with a large left neck nodule and neck tightnes...

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Main Authors: Evelyn O. Ilori, Hanan Hamdan, Venetia Sarode, Iram Hussain, Ana Islam, Justin A. Bishop
Format: Article
Language:English
Published: BMC 2025-07-01
Series:Diagnostic Pathology
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Online Access:https://doi.org/10.1186/s13000-025-01682-7
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author Evelyn O. Ilori
Hanan Hamdan
Venetia Sarode
Iram Hussain
Ana Islam
Justin A. Bishop
author_facet Evelyn O. Ilori
Hanan Hamdan
Venetia Sarode
Iram Hussain
Ana Islam
Justin A. Bishop
author_sort Evelyn O. Ilori
collection DOAJ
description Abstract Background Spindle epithelial tumor with thymus-like differentiation or elements (SETTLE) is a rare and malignant biphasic tumor of the thyroid that has proven challenging to diagnose by cytology. Case presentation A 20-year-old-male presented with a large left neck nodule and neck tightness. Laboratory tests were normal. Computed Tomography (CT) of the neck revealed a thyroid mass involving the isthmus and entire left lobe. Ultrasound-guided fine needle aspiration of the mass demonstrated sheets and clusters of oval to spindle cells with associated fibrovascular cores. The cells had moderately pleomorphic nuclei with stippled chromatin, inconspicuous nucleoli, and minimal cytoplasm, and a diagnosis of medullary thyroid carcinoma was made. A total thyroidectomy and neck dissection was performed. Morphological and immunohistochemical evaluation of the specimen resulted in a diagnosis of SETTLE with angioinvasion and involved lymph nodes. Conclusion SETTLE has several mimics cytologically, such as medullary thyroid carcinoma, that makes accurate pre-operative diagnosis challenging. We present a review of cytology information from literature, highlighting features that differentiates SETTLE from its mimics.
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spelling doaj-art-9ed321ef78954d588ec8ca2da744ae7f2025-08-20T03:42:23ZengBMCDiagnostic Pathology1746-15962025-07-012011610.1186/s13000-025-01682-7Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literatureEvelyn O. Ilori0Hanan Hamdan1Venetia Sarode2Iram Hussain3Ana Islam4Justin A. Bishop5Department of Pathology, University of Texas Southwestern Medical CenterDepartment of Pathology, University of Texas Southwestern Medical CenterPropathDepartment of Internal Medicine, University of Texas Southwestern Medical CenterDepartment of Surgery, University of Texas Southwestern Medical CenterDepartment of Pathology, University of Texas Southwestern Medical CenterAbstract Background Spindle epithelial tumor with thymus-like differentiation or elements (SETTLE) is a rare and malignant biphasic tumor of the thyroid that has proven challenging to diagnose by cytology. Case presentation A 20-year-old-male presented with a large left neck nodule and neck tightness. Laboratory tests were normal. Computed Tomography (CT) of the neck revealed a thyroid mass involving the isthmus and entire left lobe. Ultrasound-guided fine needle aspiration of the mass demonstrated sheets and clusters of oval to spindle cells with associated fibrovascular cores. The cells had moderately pleomorphic nuclei with stippled chromatin, inconspicuous nucleoli, and minimal cytoplasm, and a diagnosis of medullary thyroid carcinoma was made. A total thyroidectomy and neck dissection was performed. Morphological and immunohistochemical evaluation of the specimen resulted in a diagnosis of SETTLE with angioinvasion and involved lymph nodes. Conclusion SETTLE has several mimics cytologically, such as medullary thyroid carcinoma, that makes accurate pre-operative diagnosis challenging. We present a review of cytology information from literature, highlighting features that differentiates SETTLE from its mimics.https://doi.org/10.1186/s13000-025-01682-7SETTLEThyroid tumorFNACytology
spellingShingle Evelyn O. Ilori
Hanan Hamdan
Venetia Sarode
Iram Hussain
Ana Islam
Justin A. Bishop
Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
Diagnostic Pathology
SETTLE
Thyroid tumor
FNA
Cytology
title Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
title_full Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
title_fullStr Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
title_full_unstemmed Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
title_short Diagnostic challenges of FNA cytology of SETTLE: a case report and review of the literature
title_sort diagnostic challenges of fna cytology of settle a case report and review of the literature
topic SETTLE
Thyroid tumor
FNA
Cytology
url https://doi.org/10.1186/s13000-025-01682-7
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AT iramhussain diagnosticchallengesoffnacytologyofsettleacasereportandreviewoftheliterature
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