Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR
ABSTRACT Objective Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma....
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Wiley
2025-02-01
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| Series: | Clinical and Experimental Dental Research |
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| Online Access: | https://doi.org/10.1002/cre2.70123 |
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| author | Maryam Amirchaghmaghi Atessa Pakfetrat Nooshin Mohtasham Farnaz Mohajertehran Mohammad Taghi shakeri Elahe Vazavandi |
| author_facet | Maryam Amirchaghmaghi Atessa Pakfetrat Nooshin Mohtasham Farnaz Mohajertehran Mohammad Taghi shakeri Elahe Vazavandi |
| author_sort | Maryam Amirchaghmaghi |
| collection | DOAJ |
| description | ABSTRACT Objective Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma. Materials and Methods This cross‐sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real‐time polymerase chain reaction. The data were analyzed using SPSS 20. Results The expression of survivin‐∆EX3 and survivin‐2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (p < 0.05). The mean expression of survivin‐∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, p < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (p < 0.05). There was also a significant correlation between gene expressions (p < 0.001). Conclusion The increased expression of survivin‐∆EX3 and survivin‐2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role. |
| format | Article |
| id | doaj-art-9ec0037346214040bb46ed9370dcfc2d |
| institution | Kabale University |
| issn | 2057-4347 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
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| series | Clinical and Experimental Dental Research |
| spelling | doaj-art-9ec0037346214040bb46ed9370dcfc2d2025-08-20T03:55:08ZengWileyClinical and Experimental Dental Research2057-43472025-02-01111n/an/a10.1002/cre2.70123Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCRMaryam Amirchaghmaghi0Atessa Pakfetrat1Nooshin Mohtasham2Farnaz Mohajertehran3Mohammad Taghi shakeri4Elahe Vazavandi5Oral and Maxillofacial Diseases Research Center Mashhad University of Medical Sciences Mashhad IranOral and Maxillofacial Diseases Research Center Mashhad University of Medical Sciences Mashhad IranOral and Maxillofacial Diseases Research Center Mashhad University of Medical Sciences Mashhad IranDental Research Center Mashhad University of Medical Sciences Mashhad IranSocial Determinants of Health Research Center Mashhad University of Medical Sciences Mashhad IranDepartment of Oral and Maxillofacial Medicine, School of Dentistry Kerman university of Medical Science Kerman IranABSTRACT Objective Survivin is used to determine the prognosis and clinical features of premalignant and malignant lesions. The aim of this study was to determine the correlation between the expression of survivin isoforms and clinical outcomes in oral lichen planus and oral squamous cell carcinoma. Materials and Methods This cross‐sectional study examined 119 cases, including oral squamous cell carcinoma (SCC), oral lichen planus (OLP), and healthy margins of lesions. For all lesions, survivin expression was assessed quantitatively and qualitatively using real‐time polymerase chain reaction. The data were analyzed using SPSS 20. Results The expression of survivin‐∆EX3 and survivin‐2B were quantitatively and qualitatively higher in SCC and OLP cases than in healthy mucosa (p < 0.05). The mean expression of survivin‐∆EX3 in erosive OLP (4.95 ± 4.41) was higher than that in nonerosive OLP (2.13 ± 3.32, p < 0.05). Moreover, the mean expression of both genes was significantly higher in different grades of SCC compared to healthy mucosa (p < 0.05). There was also a significant correlation between gene expressions (p < 0.001). Conclusion The increased expression of survivin‐∆EX3 and survivin‐2B in OSCC correlates with tumor progression and advanced clinical stages, suggesting a potential prognostic role.https://doi.org/10.1002/cre2.70123gene expressionneoplasmoral lichen planussquamous cell carcinomasurvivin |
| spellingShingle | Maryam Amirchaghmaghi Atessa Pakfetrat Nooshin Mohtasham Farnaz Mohajertehran Mohammad Taghi shakeri Elahe Vazavandi Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR Clinical and Experimental Dental Research gene expression neoplasm oral lichen planus squamous cell carcinoma survivin |
| title | Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR |
| title_full | Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR |
| title_fullStr | Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR |
| title_full_unstemmed | Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR |
| title_short | Comparative Study of the Expressions of Nuclear (∆EX3) and Cytoplasmic (2B) Survivins in Oral Squamous Cell Carcinoma and Oral Lichen Planus Using Real‐Time PCR |
| title_sort | comparative study of the expressions of nuclear ∆ex3 and cytoplasmic 2b survivins in oral squamous cell carcinoma and oral lichen planus using real time pcr |
| topic | gene expression neoplasm oral lichen planus squamous cell carcinoma survivin |
| url | https://doi.org/10.1002/cre2.70123 |
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