Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers

Clematis lanuginosa, a valuable ornamental plant in Zhejiang Province, China, produces flowers that are blue–purple, a rare flower colour. In this study, to explore the anthocyanin synthesis mechanism involved in flower colour formation in C. lanuginosa, metabolome, and transcriptome sequencing was...

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Main Authors: Renjuan Qian, Youju Ye, Xiaohua Ma, Handong Gao, Qingdi Hu, Jian Zheng
Format: Article
Language:English
Published: Maximum Academic Press 2025-01-01
Series:Ornamental Plant Research
Subjects:
Online Access:https://www.maxapress.com/article/doi/10.48130/opr-0024-0031
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author Renjuan Qian
Youju Ye
Xiaohua Ma
Handong Gao
Qingdi Hu
Jian Zheng
author_facet Renjuan Qian
Youju Ye
Xiaohua Ma
Handong Gao
Qingdi Hu
Jian Zheng
author_sort Renjuan Qian
collection DOAJ
description Clematis lanuginosa, a valuable ornamental plant in Zhejiang Province, China, produces flowers that are blue–purple, a rare flower colour. In this study, to explore the anthocyanin synthesis mechanism involved in flower colour formation in C. lanuginosa, metabolome, and transcriptome sequencing was performed at six flower development stages. Metabolome analysis revealed 25 anthocyanin compounds and 22 differentially expressed metabolites. Cyanidin-3,5-O-diglucoside, cyanidin-3,5,3'-O-triglucoside, cyanidin-3-O-rutinoside-5-O-glucoside, delphinidin-3-O-glucoside, and petunidin-3-O-glucoside may promote the formation of blue–purple colour in flowers. The combined analysis results revealed that the transcript41913_f2p0_1152 gene (MYB-like) may be a key gene in C. lanuginosa blue–purple flower colour development. These results provide the basis for further research on the blue–purple flower colour of C. lanuginosa.
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issn 2769-2094
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publishDate 2025-01-01
publisher Maximum Academic Press
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series Ornamental Plant Research
spelling doaj-art-9ebdca6fbffc46a79c9dd55ff38f53402025-08-20T02:12:33ZengMaximum Academic PressOrnamental Plant Research2769-20942025-01-01511910.48130/opr-0024-0031opr-0024-0031Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowersRenjuan Qian0Youju Ye1Xiaohua Ma2Handong Gao3Qingdi Hu4Jian Zheng5Zhejiang Institute of Subtropical Crops, Wenzhou Key Laboratory of Resource Plant Innovation and Utilization, Wenzhou 325005, Zhejiang, ChinaZhejiang Institute of Subtropical Crops, Wenzhou Key Laboratory of Resource Plant Innovation and Utilization, Wenzhou 325005, Zhejiang, ChinaZhejiang Institute of Subtropical Crops, Wenzhou Key Laboratory of Resource Plant Innovation and Utilization, Wenzhou 325005, Zhejiang, ChinaCollege of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaZhejiang Institute of Subtropical Crops, Wenzhou Key Laboratory of Resource Plant Innovation and Utilization, Wenzhou 325005, Zhejiang, ChinaZhejiang Institute of Subtropical Crops, Wenzhou Key Laboratory of Resource Plant Innovation and Utilization, Wenzhou 325005, Zhejiang, ChinaClematis lanuginosa, a valuable ornamental plant in Zhejiang Province, China, produces flowers that are blue–purple, a rare flower colour. In this study, to explore the anthocyanin synthesis mechanism involved in flower colour formation in C. lanuginosa, metabolome, and transcriptome sequencing was performed at six flower development stages. Metabolome analysis revealed 25 anthocyanin compounds and 22 differentially expressed metabolites. Cyanidin-3,5-O-diglucoside, cyanidin-3,5,3'-O-triglucoside, cyanidin-3-O-rutinoside-5-O-glucoside, delphinidin-3-O-glucoside, and petunidin-3-O-glucoside may promote the formation of blue–purple colour in flowers. The combined analysis results revealed that the transcript41913_f2p0_1152 gene (MYB-like) may be a key gene in C. lanuginosa blue–purple flower colour development. These results provide the basis for further research on the blue–purple flower colour of C. lanuginosa.https://www.maxapress.com/article/doi/10.48130/opr-0024-0031clematis lanuginosaflower colourblue–purplecombined analysis
spellingShingle Renjuan Qian
Youju Ye
Xiaohua Ma
Handong Gao
Qingdi Hu
Jian Zheng
Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
Ornamental Plant Research
clematis lanuginosa
flower colour
blue–purple
combined analysis
title Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
title_full Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
title_fullStr Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
title_full_unstemmed Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
title_short Targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue–purple colour development in Clematis lanuginosa flowers
title_sort targeted metabolome and transcriptome analysis reveals the key metabolites and genes influencing blue purple colour development in clematis lanuginosa flowers
topic clematis lanuginosa
flower colour
blue–purple
combined analysis
url https://www.maxapress.com/article/doi/10.48130/opr-0024-0031
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