Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn
A new (Reversed Phase- High Performance Liquid chromatography) RP-HPLC method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of antioxidants present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on usi...
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University of Baghdad, College of Science for Women
2018-12-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2942 |
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| description | A new (Reversed Phase- High Performance Liquid chromatography) RP-HPLC method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of antioxidants present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting antioxidants. Limit of detection in μg/ml of Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, Dephinidin-3-glucoside were113.8294×10-6,123.0453×10-6,70.3681×10-6,59.6730×10-6,148.1710×10-6,and125.3481×10-6 respectively. The concentration of antioxidants found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, and Dephinidin-3-glucoside are 258.3 μg/g, 225.51 μg/g, 154.975 μg/g, 111.407 μg/g, 439.442 μg/g, and 185.729 μg/g respectively. |
| format | Article |
| id | doaj-art-9ebc96cad9734e95956195a0301ff596 |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2018-12-01 |
| publisher | University of Baghdad, College of Science for Women |
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| series | مجلة بغداد للعلوم |
| spelling | doaj-art-9ebc96cad9734e95956195a0301ff5962025-08-20T03:57:03ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862018-12-0112110.21123/bsj.12.1.119-126Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia LinnBaghdad Science JournalA new (Reversed Phase- High Performance Liquid chromatography) RP-HPLC method with Ultraviolet-Visible spectrophotometry has been optimized and validated for the simultaneous extraction and determination of antioxidants present in Iraqi calyces of Hibiscus Sabdraffia Linn. The method is based on using ultrasonic bath for extracting antioxidants. Limit of detection in μg/ml of Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, Dephinidin-3-glucoside were113.8294×10-6,123.0453×10-6,70.3681×10-6,59.6730×10-6,148.1710×10-6,and125.3481×10-6 respectively. The concentration of antioxidants found in dry spacemen of calyces of Iraqi Hibiscus Sabdraffia Linn. under study: Vitamin C, Sabdaretine, Gossypetine, Hibiscetine, Anthocyanins, and Dephinidin-3-glucoside are 258.3 μg/g, 225.51 μg/g, 154.975 μg/g, 111.407 μg/g, 439.442 μg/g, and 185.729 μg/g respectively.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2942antioxidants, Hibiscus Sabdraffia Linn |
| spellingShingle | Baghdad Science Journal Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn مجلة بغداد للعلوم antioxidants, Hibiscus Sabdraffia Linn |
| title | Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn |
| title_full | Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn |
| title_fullStr | Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn |
| title_full_unstemmed | Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn |
| title_short | Optimization and Validation of RP-HPLC-UV/VIS Method for Determination Some Antioxidants in Dry Calyces of Iraqi Hibiscus Sabdraffia Linn |
| title_sort | optimization and validation of rp hplc uv vis method for determination some antioxidants in dry calyces of iraqi hibiscus sabdraffia linn |
| topic | antioxidants, Hibiscus Sabdraffia Linn |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2942 |
| work_keys_str_mv | AT baghdadsciencejournal optimizationandvalidationofrphplcuvvismethodfordeterminationsomeantioxidantsindrycalycesofiraqihibiscussabdraffialinn |