Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster
ABSTRACT Oxidative stress contributes to cell aging and is implicated in diseases such as Alzheimer's disease, Parkinson's syndrome, atherosclerosis, and cancer. As a classic model for studying aging, Drosophila is also widely used in oxidative stress research due to its genetic tractabili...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Future Postharvest and Food |
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| Online Access: | https://doi.org/10.1002/fpf2.70003 |
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| author | Quanyong Wu |
| author_facet | Quanyong Wu |
| author_sort | Quanyong Wu |
| collection | DOAJ |
| description | ABSTRACT Oxidative stress contributes to cell aging and is implicated in diseases such as Alzheimer's disease, Parkinson's syndrome, atherosclerosis, and cancer. As a classic model for studying aging, Drosophila is also widely used in oxidative stress research due to its genetic tractability and physiological relevance. However, researchers often face challenges in selecting appropriate methods to characterize oxidative stress damage due to the diversity of available techniques and the complexity of oxidative stress mechanisms. To address this gap, this guideline provides a comprehensive summary of commonly used research methods for studying oxidative stress damage using Drosophila. It includes detailed explanations of basic principles and specific operating procedures, covering aspects such as lifespan analysis, ROS content, antioxidant enzyme activity, lipid peroxidation, protein oxidation, DNA damage, and inflammation levels. By offering an organized and practical reference, this guideline serves as a critical resource for researchers in method selection and experimental design, thus advancing the field of oxidative stress research. |
| format | Article |
| id | doaj-art-9eb992f01e6d4701b4765b260ff202a9 |
| institution | Kabale University |
| issn | 2837-6846 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Future Postharvest and Food |
| spelling | doaj-art-9eb992f01e6d4701b4765b260ff202a92025-08-20T03:40:57ZengWileyFuture Postharvest and Food2837-68462025-06-012212413810.1002/fpf2.70003Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogasterQuanyong Wu0Nutrition and Bromatology Group Department of Analytical Chemistry and Food Science Instituto de Agroecoloxía e Alimentación (IAA) – CITEXVI Universidade de Vigo Vigo SpainABSTRACT Oxidative stress contributes to cell aging and is implicated in diseases such as Alzheimer's disease, Parkinson's syndrome, atherosclerosis, and cancer. As a classic model for studying aging, Drosophila is also widely used in oxidative stress research due to its genetic tractability and physiological relevance. However, researchers often face challenges in selecting appropriate methods to characterize oxidative stress damage due to the diversity of available techniques and the complexity of oxidative stress mechanisms. To address this gap, this guideline provides a comprehensive summary of commonly used research methods for studying oxidative stress damage using Drosophila. It includes detailed explanations of basic principles and specific operating procedures, covering aspects such as lifespan analysis, ROS content, antioxidant enzyme activity, lipid peroxidation, protein oxidation, DNA damage, and inflammation levels. By offering an organized and practical reference, this guideline serves as a critical resource for researchers in method selection and experimental design, thus advancing the field of oxidative stress research.https://doi.org/10.1002/fpf2.70003Drosophila melanogasterinflammationlifespanmethodsoxidative stressROS |
| spellingShingle | Quanyong Wu Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster Future Postharvest and Food Drosophila melanogaster inflammation lifespan methods oxidative stress ROS |
| title | Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster |
| title_full | Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster |
| title_fullStr | Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster |
| title_full_unstemmed | Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster |
| title_short | Guidelines for Studying the Oxidative Stress Damage Model on Drosophila melanogaster |
| title_sort | guidelines for studying the oxidative stress damage model on drosophila melanogaster |
| topic | Drosophila melanogaster inflammation lifespan methods oxidative stress ROS |
| url | https://doi.org/10.1002/fpf2.70003 |
| work_keys_str_mv | AT quanyongwu guidelinesforstudyingtheoxidativestressdamagemodelondrosophilamelanogaster |