Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry
The remarkable advancement of nanotechnology has triggered enormous production of metal nanoparticles and nanomaterials for diverse applications in clinical diagnostics and biomedical research. Nanotechnology has facilitated understanding and analysing nanotoxicology in a holistic approach. Iron nan...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Nanotechnology |
| Online Access: | http://dx.doi.org/10.1155/2016/2547467 |
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| author | Venkatachalam Deepa Parvathi Kalyanaraman Rajagopal Ravindran Sumitha |
| author_facet | Venkatachalam Deepa Parvathi Kalyanaraman Rajagopal Ravindran Sumitha |
| author_sort | Venkatachalam Deepa Parvathi |
| collection | DOAJ |
| description | The remarkable advancement of nanotechnology has triggered enormous production of metal nanoparticles and nanomaterials for diverse applications in clinical diagnostics and biomedical research. Nanotechnology has facilitated understanding and analysing nanotoxicology in a holistic approach. Iron nanoparticles have been of special interest in recent research owing to their dynamic, paramagnetic, and catalytic properties. Research studies (in vitro model) have demonstrated the lack of toxicity in nanoiron. The present study design involves in vivo toxicity assessment of nanoiron at specific concentrations of 0.1 mM, 1 mM, 5 mM, and 10 mM in Drosophila. DNA fragmentation assay in exposed and F1 population showed first-line toxicity to flies. Viability and reproductive ability were assessed at 24-hour and 48-hour intervals and thus indicated no statistical significance between the exposed and control groups. The wing spot assay has expressed transparent lack of toxicity in the studied concentrations of nanoiron. Protein profiling has demonstrated that the protein profiles have been intact in the larvae which confirm lack of toxicity of nanoiron. This leads to concluding that nanoiron at the defined concentrations is neither genotoxic nor mutagenic. |
| format | Article |
| id | doaj-art-9eb492f34c024246a40d844e4be35802 |
| institution | Kabale University |
| issn | 1687-9503 1687-9511 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nanotechnology |
| spelling | doaj-art-9eb492f34c024246a40d844e4be358022025-02-03T01:02:42ZengWileyJournal of Nanotechnology1687-95031687-95112016-01-01201610.1155/2016/25474672547467Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to NanodosimetryVenkatachalam Deepa Parvathi0Kalyanaraman Rajagopal1Ravindran Sumitha2Department of Human Genetics, Sri Ramachandra University, Chennai, Tamil Nadu 600116, IndiaSchool of Life Sciences, Vels University, Chennai, Tamil Nadu 600117, IndiaDepartment of Biomedical Sciences, Sri Ramachandra University, Chennai, Tamil Nadu 600116, IndiaThe remarkable advancement of nanotechnology has triggered enormous production of metal nanoparticles and nanomaterials for diverse applications in clinical diagnostics and biomedical research. Nanotechnology has facilitated understanding and analysing nanotoxicology in a holistic approach. Iron nanoparticles have been of special interest in recent research owing to their dynamic, paramagnetic, and catalytic properties. Research studies (in vitro model) have demonstrated the lack of toxicity in nanoiron. The present study design involves in vivo toxicity assessment of nanoiron at specific concentrations of 0.1 mM, 1 mM, 5 mM, and 10 mM in Drosophila. DNA fragmentation assay in exposed and F1 population showed first-line toxicity to flies. Viability and reproductive ability were assessed at 24-hour and 48-hour intervals and thus indicated no statistical significance between the exposed and control groups. The wing spot assay has expressed transparent lack of toxicity in the studied concentrations of nanoiron. Protein profiling has demonstrated that the protein profiles have been intact in the larvae which confirm lack of toxicity of nanoiron. This leads to concluding that nanoiron at the defined concentrations is neither genotoxic nor mutagenic.http://dx.doi.org/10.1155/2016/2547467 |
| spellingShingle | Venkatachalam Deepa Parvathi Kalyanaraman Rajagopal Ravindran Sumitha Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry Journal of Nanotechnology |
| title | Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry |
| title_full | Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry |
| title_fullStr | Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry |
| title_full_unstemmed | Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry |
| title_short | Standardization of Alternative Methods for Nanogenotoxicity Testing in Drosophila melanogaster Using Iron Nanoparticles: A Promising Link to Nanodosimetry |
| title_sort | standardization of alternative methods for nanogenotoxicity testing in drosophila melanogaster using iron nanoparticles a promising link to nanodosimetry |
| url | http://dx.doi.org/10.1155/2016/2547467 |
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