Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans

Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans

Summary: Here, we present a protocol for isolating microvessels from fresh or snap-frozen brain tissue from mice and humans, followed by visualization of RNA utilizing RNAscope hybridization for quantification of mRNA. We describe the steps for sample preparation and isolation, fixation, and hybridi...

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Bibliographic Details
Main Authors: Olivia M. Osborne, Oandy Naranjo, Silvia Torices, Sarah Schmidlin, Destiny Tiburcio, Minseon Park, Michal Toborek
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Classification Description
Cell isolation
Microscopy
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724006956
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Summary:Summary: Here, we present a protocol for isolating microvessels from fresh or snap-frozen brain tissue from mice and humans, followed by visualization of RNA utilizing RNAscope hybridization for quantification of mRNA. We describe the steps for sample preparation and isolation, fixation, and hybridization. This protocol was specifically designed to integrate with RNAscope in situ hybridization. Although the protocol was developed in a mouse model, it can be optimized for use in other organisms, including human brain samples. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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