Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer

Objective To construct prognostic model of disulfidptosis-related LncRNA (DRLncs) and explore the mechanism of LncRNA-small nucleolar RNA host genes(SNHG4) in promoting the progression of gastric cancer. Methods The multi-omics features of disulfidptosis genes in gastric cancer were first analyzed b...

Full description

Saved in:
Bibliographic Details
Main Author: CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong
Format: Article
Language:zho
Published: Editorial Office of Journal of Surgery Concepts & Practice 2024-09-01
Series:Waike lilun yu shijian
Subjects:
Online Access:https://www.qk.sjtu.edu.cn/jscp/fileup/1007-9610/PDF/1737619549278-359779908.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832590621977608192
author CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong
author_facet CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong
author_sort CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong
collection DOAJ
description Objective To construct prognostic model of disulfidptosis-related LncRNA (DRLncs) and explore the mechanism of LncRNA-small nucleolar RNA host genes(SNHG4) in promoting the progression of gastric cancer. Methods The multi-omics features of disulfidptosis genes in gastric cancer were first analyzed based on The Cancer Genome Atlas(TCGA) database. DRLncs were further screened, and disulfidptosis related gene recurrence score (DRG-RS) model was constructed to predict the overall survival(OS) and correlation with the clinical characteristics of gastric cancer patients. Differential gene analysis, functional enrichment and the correlation between chemotherapy efficacy in high- and low-risk groups were analyzed. Cell-counting kit-8 (CCK-8) assay and migration assay confirmed that LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells. Western Blotting assay was used to detect the signaling pathway regulated by LncRNA-SNHG4. Results DRG-RS model including 8 DRLncs was successfully constructed, showing good performance in predicting OS of the patients with gastric cancer. DRG-RS model was significantly correlated with tumor-related signaling pathways, clinical characteristics and drug sensitivity of gastric cancer patients. LncRNA-SNHG4 expression was significantly increased in gastric cancer. LncRNA-SNHG4 promoted the proliferation and migration of gastric cancer cells. LncRNA-SNHG4 promoted the activation of Wnt signaling pathway in gastric cancer cells. Conclusions The DRG-RS model constructed in our study can stratify the prognosis of gastric cancer, and is closely related to malignant characteristics and chemotherapy efficacy of gastric cancer patients. The expression of LncRNA-SNHG4 significantly increased in gastric cancer tissues, and LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells through the Wnt signaling pathway, suggesting that LncRNA-SNHG4 may play an important role in the progression of gastric cancer.
format Article
id doaj-art-9eb3f40298004fe38fa1289f4e6d72cc
institution Kabale University
issn 1007-9610
language zho
publishDate 2024-09-01
publisher Editorial Office of Journal of Surgery Concepts & Practice
record_format Article
series Waike lilun yu shijian
spelling doaj-art-9eb3f40298004fe38fa1289f4e6d72cc2025-01-23T10:48:45ZzhoEditorial Office of Journal of Surgery Concepts & PracticeWaike lilun yu shijian1007-96102024-09-01290541442510.16139/j.1007-9610.2024.05.09Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancerCHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong0Department of Oncology, Shanghai Minhang Cancer Hospital, Shanghai 200240, ChinaObjective To construct prognostic model of disulfidptosis-related LncRNA (DRLncs) and explore the mechanism of LncRNA-small nucleolar RNA host genes(SNHG4) in promoting the progression of gastric cancer. Methods The multi-omics features of disulfidptosis genes in gastric cancer were first analyzed based on The Cancer Genome Atlas(TCGA) database. DRLncs were further screened, and disulfidptosis related gene recurrence score (DRG-RS) model was constructed to predict the overall survival(OS) and correlation with the clinical characteristics of gastric cancer patients. Differential gene analysis, functional enrichment and the correlation between chemotherapy efficacy in high- and low-risk groups were analyzed. Cell-counting kit-8 (CCK-8) assay and migration assay confirmed that LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells. Western Blotting assay was used to detect the signaling pathway regulated by LncRNA-SNHG4. Results DRG-RS model including 8 DRLncs was successfully constructed, showing good performance in predicting OS of the patients with gastric cancer. DRG-RS model was significantly correlated with tumor-related signaling pathways, clinical characteristics and drug sensitivity of gastric cancer patients. LncRNA-SNHG4 expression was significantly increased in gastric cancer. LncRNA-SNHG4 promoted the proliferation and migration of gastric cancer cells. LncRNA-SNHG4 promoted the activation of Wnt signaling pathway in gastric cancer cells. Conclusions The DRG-RS model constructed in our study can stratify the prognosis of gastric cancer, and is closely related to malignant characteristics and chemotherapy efficacy of gastric cancer patients. The expression of LncRNA-SNHG4 significantly increased in gastric cancer tissues, and LncRNA-SNHG4 promoted the malignant phenotype of gastric cancer cells through the Wnt signaling pathway, suggesting that LncRNA-SNHG4 may play an important role in the progression of gastric cancer.https://www.qk.sjtu.edu.cn/jscp/fileup/1007-9610/PDF/1737619549278-359779908.pdf|gastric cancer|disulfidptosis|disulfidptosis-related long non-coding rna(lncrna)|lncrna-small nucleolar rna host gene (snhg4)|wnt signaling pathway
spellingShingle CHU Yudan, SUN Haidong, CUI Ran, ZHENG Hong
Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
Waike lilun yu shijian
|gastric cancer|disulfidptosis|disulfidptosis-related long non-coding rna(lncrna)|lncrna-small nucleolar rna host gene (snhg4)|wnt signaling pathway
title Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
title_full Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
title_fullStr Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
title_full_unstemmed Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
title_short Expression and clinical significance of disulfidptosis-related LncRNA in gastric cancer
title_sort expression and clinical significance of disulfidptosis related lncrna in gastric cancer
topic |gastric cancer|disulfidptosis|disulfidptosis-related long non-coding rna(lncrna)|lncrna-small nucleolar rna host gene (snhg4)|wnt signaling pathway
url https://www.qk.sjtu.edu.cn/jscp/fileup/1007-9610/PDF/1737619549278-359779908.pdf
work_keys_str_mv AT chuyudansunhaidongcuiranzhenghong expressionandclinicalsignificanceofdisulfidptosisrelatedlncrnaingastriccancer