A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification
Abstract Breast cancer is the most prevalent malignancy among women worldwide. With rapid societal development, there has been a consistent increase in breast cancer incidence rates annually, accompanied by a progressively younger age of onset. Consequently, there is an urgent need to develop a rapi...
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BMC
2025-04-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03340-y |
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| author | Zan Yu Wenxin Liu Lei Wang |
| author_facet | Zan Yu Wenxin Liu Lei Wang |
| author_sort | Zan Yu |
| collection | DOAJ |
| description | Abstract Breast cancer is the most prevalent malignancy among women worldwide. With rapid societal development, there has been a consistent increase in breast cancer incidence rates annually, accompanied by a progressively younger age of onset. Consequently, there is an urgent need to develop a rapid, sensitive, and highly specific approach for the identification of BRCA-1. In this study, we successfully devised a heterogeneous system based on G-quadruplex DNAzyme for label-free and enzyme-free visual detection of BRCA-1. Moreover, the distinctive G-quadruplex (G4) DNAzyme ensures precise and accurate detection through enzyme-free amplification, colorimetric detection, and catalytic hairpin assembly mechanisms. The utilization of this heterogeneous system effectively minimizes background interference while exhibiting exceptional sensitivity and accuracy with an impressive detection limit as low as 0.6 pM. Furthermore, real sample analysis yielded an area under the ROC curve value of 0.9825 which demonstrates its robust performance for early screening purposes when integrated with smartphone-assisted analysis, thus showcasing its potential for widespread application in early breast cancer diagnosis. |
| format | Article |
| id | doaj-art-9eb15adc063042a3b4aa9fcdc65cc0b3 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-9eb15adc063042a3b4aa9fcdc65cc0b32025-08-20T03:04:53ZengBMCJournal of Nanobiotechnology1477-31552025-04-012311910.1186/s12951-025-03340-yA smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplificationZan Yu0Wenxin Liu1Lei Wang2Zhanjiang Central Hospital, Guangdong medical universityZhanjiang Central Hospital, Guangdong medical universityZhanjiang Central Hospital, Guangdong medical universityAbstract Breast cancer is the most prevalent malignancy among women worldwide. With rapid societal development, there has been a consistent increase in breast cancer incidence rates annually, accompanied by a progressively younger age of onset. Consequently, there is an urgent need to develop a rapid, sensitive, and highly specific approach for the identification of BRCA-1. In this study, we successfully devised a heterogeneous system based on G-quadruplex DNAzyme for label-free and enzyme-free visual detection of BRCA-1. Moreover, the distinctive G-quadruplex (G4) DNAzyme ensures precise and accurate detection through enzyme-free amplification, colorimetric detection, and catalytic hairpin assembly mechanisms. The utilization of this heterogeneous system effectively minimizes background interference while exhibiting exceptional sensitivity and accuracy with an impressive detection limit as low as 0.6 pM. Furthermore, real sample analysis yielded an area under the ROC curve value of 0.9825 which demonstrates its robust performance for early screening purposes when integrated with smartphone-assisted analysis, thus showcasing its potential for widespread application in early breast cancer diagnosis.https://doi.org/10.1186/s12951-025-03340-yBRCA-1HeterogeneousG-quadruplex DNAzymeCatalytic hairpin assembly |
| spellingShingle | Zan Yu Wenxin Liu Lei Wang A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification Journal of Nanobiotechnology BRCA-1 Heterogeneous G-quadruplex DNAzyme Catalytic hairpin assembly |
| title | A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| title_full | A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| title_fullStr | A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| title_full_unstemmed | A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| title_short | A smartphone-colorimetric dual-mode diagnosis method for breast cancer detection of BRCA-1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| title_sort | smartphone colorimetric dual mode diagnosis method for breast cancer detection of brca 1 gene base on silver plated substrate and catalytic hairpin assembly amplification |
| topic | BRCA-1 Heterogeneous G-quadruplex DNAzyme Catalytic hairpin assembly |
| url | https://doi.org/10.1186/s12951-025-03340-y |
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