Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea
Terpenoids play important biological and ecological roles in plant defense against the pests’ stress, and geranyllinalool synthase (GES) is a key enzyme in the biosynthetic pathway of terpenoids. In order to understand the function of GES in Brassica oleracea, we cloned the BoGES gene by polymerase...
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Zhejiang University Press
2022-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.12.031 |
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| author | WANG Yiping GE Yang ZHANG Yixin Asim MUNAWAR ZHANG Yadong MAO Lijuan ZHU Zengrong ZHOU Wenwu |
| author_facet | WANG Yiping GE Yang ZHANG Yixin Asim MUNAWAR ZHANG Yadong MAO Lijuan ZHU Zengrong ZHOU Wenwu |
| author_sort | WANG Yiping |
| collection | DOAJ |
| description | Terpenoids play important biological and ecological roles in plant defense against the pests’ stress, and geranyllinalool synthase (GES) is a key enzyme in the biosynthetic pathway of terpenoids. In order to understand the function of GES in Brassica oleracea, we cloned the BoGES gene by polymerase chain reaction and analyzed its expression levels under different biological stresses, after that we studied the recombinant protein by prokaryotic expression and analyzed its biochemical functions. The results showed that the amino acid sequence of BoGES protein was highly conserved in Brassicaceae, indicating its conserved function in these plants. The expression of BoGES gene could be significantly induced by diamondback moth (DBM) damage, Pst DC3000 infection, and salicylic acid and methyl jasmonate treatments, suggesting its functions in response to the biological stresses. BoGES protein could catalyze the formation of geranyllinalool from the substrate geranylgeranyl diphosphate (GGPP). Moreover, we measured the choice response of the parasitoid wasps to different concentrations of geranyllinalool via the Y-tube olfactometer, and found that this chemical could attract the parasitoid wasps. In conclusion, this study systemically analyzed the function of BoGES gene in B. oleracea, which could provide the scientific basis for further study of the biological and ecological functions of terpene synthase and terpenoids in Brassicaceae plants. |
| format | Article |
| id | doaj-art-9eae4827ed734ab69848af91793806be |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2022-10-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-9eae4827ed734ab69848af91793806be2025-08-20T03:16:03ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552022-10-014858359310.3785/j.issn.1008-9209.2021.12.03110089209Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleraceaWANG YipingGE YangZHANG YixinAsim MUNAWARZHANG YadongMAO LijuanZHU ZengrongZHOU WenwuTerpenoids play important biological and ecological roles in plant defense against the pests’ stress, and geranyllinalool synthase (GES) is a key enzyme in the biosynthetic pathway of terpenoids. In order to understand the function of GES in Brassica oleracea, we cloned the BoGES gene by polymerase chain reaction and analyzed its expression levels under different biological stresses, after that we studied the recombinant protein by prokaryotic expression and analyzed its biochemical functions. The results showed that the amino acid sequence of BoGES protein was highly conserved in Brassicaceae, indicating its conserved function in these plants. The expression of BoGES gene could be significantly induced by diamondback moth (DBM) damage, Pst DC3000 infection, and salicylic acid and methyl jasmonate treatments, suggesting its functions in response to the biological stresses. BoGES protein could catalyze the formation of geranyllinalool from the substrate geranylgeranyl diphosphate (GGPP). Moreover, we measured the choice response of the parasitoid wasps to different concentrations of geranyllinalool via the Y-tube olfactometer, and found that this chemical could attract the parasitoid wasps. In conclusion, this study systemically analyzed the function of BoGES gene in B. oleracea, which could provide the scientific basis for further study of the biological and ecological functions of terpene synthase and terpenoids in Brassicaceae plants.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.12.031<italic>Brassica oleracea</italic> terpene synthasebiological stressgeranyllinaloolgeranyllinalool synthase |
| spellingShingle | WANG Yiping GE Yang ZHANG Yixin Asim MUNAWAR ZHANG Yadong MAO Lijuan ZHU Zengrong ZHOU Wenwu Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea 浙江大学学报. 农业与生命科学版 <italic>Brassica oleracea</italic> terpene synthase biological stress geranyllinalool geranyllinalool synthase |
| title | Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea |
| title_full | Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea |
| title_fullStr | Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea |
| title_full_unstemmed | Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea |
| title_short | Cloning and functional analysis of geranyllinalool synthase gene from Brassica oleracea |
| title_sort | cloning and functional analysis of geranyllinalool synthase gene from brassica oleracea |
| topic | <italic>Brassica oleracea</italic> terpene synthase biological stress geranyllinalool geranyllinalool synthase |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2021.12.031 |
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