Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study
Background: To optimize a novel composite scaffold comprising of nano phosphorylated pullulan incorporated carboxymethyl chitosan (nPP-CMC) and to evaluate its cell viability and proliferation in human dental pulp stem cells (hDPSCs). Methodology: Nano phosphorylated pullulan (nPP) in concentrations...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
|
| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_1222_24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850195050724589568 |
|---|---|
| author | M Sornamalar C Dhanavel G Balavaishnavi Shahana Shajahan Kavya Baskar S Vijaya Raja |
| author_facet | M Sornamalar C Dhanavel G Balavaishnavi Shahana Shajahan Kavya Baskar S Vijaya Raja |
| author_sort | M Sornamalar |
| collection | DOAJ |
| description | Background:
To optimize a novel composite scaffold comprising of nano phosphorylated pullulan incorporated carboxymethyl chitosan (nPP-CMC) and to evaluate its cell viability and proliferation in human dental pulp stem cells (hDPSCs).
Methodology:
Nano phosphorylated pullulan (nPP) in concentrations of 200 mg, 400 mg, 600 mg, 800 mg, and 1 g was vortexed in 20 mL of distilled water, followed by 30 mins of ultrasonication for even dispersion. 1 g of CMC was added to each nPP mixture, blended for 1 hour to form a hydrogel, and freeze-dried for 18 hours at -20°C to yield nPP-CMC scaffolds in ratios of 1:5, 2:5, 3:5, 4:5, and 1:1. These scaffolds were stored at room temperature. The scaffolds were characterized using SEM-EDX, FT-IR, and XRD. The optimized nPP-CMC (group 1), CMC (group 2), and osteogenic medium (group 3) were tested for cell viability with hDPSCs using the MTT assay at 0, 7, 14, and 21 days.
Results:
Based on the results of MTT assay, the cell viability and proliferation of nPP-CMC scaffold was comparable to that of CMC in the tested time intervals of 7 and 14 days. The 4:5 nPP-CMC revealed a favourable pore size (60–180 μm) that was suitable for cell seeding and was chosen for further experiments. Cell viability/proliferation rates of hDPSCs loaded 4:5 HAnp- CMC at 21st day was significantly greater than that at 7th day (P < .05).
Conclusion:
Hence, within the limitations of this in vitro study, it could be concluded that the novel experimental scaffold comprising of nPP-CMC (4:5) is an effective biomaterial that possess good bioactivity and biocompatibility properties. Thus, nPP-CMC biomaterial will have a great futuristic potential for regeneration of pulp dentin complex. |
| format | Article |
| id | doaj-art-9eaae955ba6d4c818735c4c00c47c640 |
| institution | OA Journals |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-9eaae955ba6d4c818735c4c00c47c6402025-08-20T02:13:52ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 5S4563S456710.4103/jpbs.jpbs_1222_24Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro StudyM SornamalarC DhanavelG BalavaishnaviShahana ShajahanKavya BaskarS Vijaya RajaBackground: To optimize a novel composite scaffold comprising of nano phosphorylated pullulan incorporated carboxymethyl chitosan (nPP-CMC) and to evaluate its cell viability and proliferation in human dental pulp stem cells (hDPSCs). Methodology: Nano phosphorylated pullulan (nPP) in concentrations of 200 mg, 400 mg, 600 mg, 800 mg, and 1 g was vortexed in 20 mL of distilled water, followed by 30 mins of ultrasonication for even dispersion. 1 g of CMC was added to each nPP mixture, blended for 1 hour to form a hydrogel, and freeze-dried for 18 hours at -20°C to yield nPP-CMC scaffolds in ratios of 1:5, 2:5, 3:5, 4:5, and 1:1. These scaffolds were stored at room temperature. The scaffolds were characterized using SEM-EDX, FT-IR, and XRD. The optimized nPP-CMC (group 1), CMC (group 2), and osteogenic medium (group 3) were tested for cell viability with hDPSCs using the MTT assay at 0, 7, 14, and 21 days. Results: Based on the results of MTT assay, the cell viability and proliferation of nPP-CMC scaffold was comparable to that of CMC in the tested time intervals of 7 and 14 days. The 4:5 nPP-CMC revealed a favourable pore size (60–180 μm) that was suitable for cell seeding and was chosen for further experiments. Cell viability/proliferation rates of hDPSCs loaded 4:5 HAnp- CMC at 21st day was significantly greater than that at 7th day (P < .05). Conclusion: Hence, within the limitations of this in vitro study, it could be concluded that the novel experimental scaffold comprising of nPP-CMC (4:5) is an effective biomaterial that possess good bioactivity and biocompatibility properties. Thus, nPP-CMC biomaterial will have a great futuristic potential for regeneration of pulp dentin complex.https://journals.lww.com/10.4103/jpbs.jpbs_1222_24carboxymethylchitosanhuman dental pulp stem cellsnano phosphorylated pullulanpulpal cell viability |
| spellingShingle | M Sornamalar C Dhanavel G Balavaishnavi Shahana Shajahan Kavya Baskar S Vijaya Raja Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study Journal of Pharmacy and Bioallied Sciences carboxymethylchitosan human dental pulp stem cells nano phosphorylated pullulan pulpal cell viability |
| title | Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study |
| title_full | Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study |
| title_fullStr | Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study |
| title_full_unstemmed | Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study |
| title_short | Nano Phosphorylated Pullulan-Integrated Carboxymethyl Chitosan Scaffold for Regeneration of the Pulp-Dentin Complex: An In vitro Study |
| title_sort | nano phosphorylated pullulan integrated carboxymethyl chitosan scaffold for regeneration of the pulp dentin complex an in vitro study |
| topic | carboxymethylchitosan human dental pulp stem cells nano phosphorylated pullulan pulpal cell viability |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_1222_24 |
| work_keys_str_mv | AT msornamalar nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy AT cdhanavel nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy AT gbalavaishnavi nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy AT shahanashajahan nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy AT kavyabaskar nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy AT svijayaraja nanophosphorylatedpullulanintegratedcarboxymethylchitosanscaffoldforregenerationofthepulpdentincomplexaninvitrostudy |