IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages

Mesenchymal stem cells (MSCs) are highly effective in the treatment of acute liver failure (ALF). The efficacy of MSCs is closely related to the inflammatory environment. Therefore, we investigated the functional changes of MSCs in response to interleukin-33 (IL-33) stimulation. The results showed t...

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Main Authors: Hui Yuan, Yuwen Li, Zihao Kong, Linya Peng, Jiali Song, Xiaoxue Hou, Wen Zhang, Rui Liu, Tiantong Feng, Chuanlong Zhu
Format: Article
Language:English
Published: Wiley 2024-01-01
Series:Stem Cells International
Online Access:http://dx.doi.org/10.1155/2024/1273099
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author Hui Yuan
Yuwen Li
Zihao Kong
Linya Peng
Jiali Song
Xiaoxue Hou
Wen Zhang
Rui Liu
Tiantong Feng
Chuanlong Zhu
author_facet Hui Yuan
Yuwen Li
Zihao Kong
Linya Peng
Jiali Song
Xiaoxue Hou
Wen Zhang
Rui Liu
Tiantong Feng
Chuanlong Zhu
author_sort Hui Yuan
collection DOAJ
description Mesenchymal stem cells (MSCs) are highly effective in the treatment of acute liver failure (ALF). The efficacy of MSCs is closely related to the inflammatory environment. Therefore, we investigated the functional changes of MSCs in response to interleukin-33 (IL-33) stimulation. The results showed that bone marrow mesenchymal stem cells (BMSCs) pretreated with IL-33 had increased CCR2 expression, targeted CCL2 in the injured liver tissue, and improved the migration ability. Under LPS stimulation, the NF-κB pathway of BMDM was activated, and its phenotype polarized to the M1-type, while BMSCs pretreated with IL-33 inhibited the NF-κB pathway and enhanced M2 macrophage polarization. The M2-type macrophages could further inhibit hepatocytes inflammation, reduce hepatocytes apoptosis, and promote hepatocytes repair. These results suggest that IL-33 can enhance the efficacy of BMSCs in ALF and provide a new strategy for cell therapy of liver diseases.
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id doaj-art-9ea1d6dc77284095bbe4d4154c3c8398
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issn 1687-9678
language English
publishDate 2024-01-01
publisher Wiley
record_format Article
series Stem Cells International
spelling doaj-art-9ea1d6dc77284095bbe4d4154c3c83982025-08-20T02:18:31ZengWileyStem Cells International1687-96782024-01-01202410.1155/2024/1273099IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 MacrophagesHui Yuan0Yuwen Li1Zihao Kong2Linya Peng3Jiali Song4Xiaoxue Hou5Wen Zhang6Rui Liu7Tiantong Feng8Chuanlong Zhu9Department of Infectious DiseaseDepartment of PediatricsDepartment of GastroenterologyDepartment of Infectious DiseaseDepartment of Infectious DiseaseDepartment of Infectious DiseaseDepartment of Infectious DiseaseDepartment of Infectious and Tropical DiseasesDepartment of Infectious DiseaseDepartment of Infectious DiseaseMesenchymal stem cells (MSCs) are highly effective in the treatment of acute liver failure (ALF). The efficacy of MSCs is closely related to the inflammatory environment. Therefore, we investigated the functional changes of MSCs in response to interleukin-33 (IL-33) stimulation. The results showed that bone marrow mesenchymal stem cells (BMSCs) pretreated with IL-33 had increased CCR2 expression, targeted CCL2 in the injured liver tissue, and improved the migration ability. Under LPS stimulation, the NF-κB pathway of BMDM was activated, and its phenotype polarized to the M1-type, while BMSCs pretreated with IL-33 inhibited the NF-κB pathway and enhanced M2 macrophage polarization. The M2-type macrophages could further inhibit hepatocytes inflammation, reduce hepatocytes apoptosis, and promote hepatocytes repair. These results suggest that IL-33 can enhance the efficacy of BMSCs in ALF and provide a new strategy for cell therapy of liver diseases.http://dx.doi.org/10.1155/2024/1273099
spellingShingle Hui Yuan
Yuwen Li
Zihao Kong
Linya Peng
Jiali Song
Xiaoxue Hou
Wen Zhang
Rui Liu
Tiantong Feng
Chuanlong Zhu
IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
Stem Cells International
title IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
title_full IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
title_fullStr IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
title_full_unstemmed IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
title_short IL-33-Pretreated Mesenchymal Stem Cells Attenuate Acute Liver Failure by Improving Homing and Polarizing M2 Macrophages
title_sort il 33 pretreated mesenchymal stem cells attenuate acute liver failure by improving homing and polarizing m2 macrophages
url http://dx.doi.org/10.1155/2024/1273099
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