Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC
Abstract The food enzyme alternansucrase (sucrose:1,6(1,3)‐α‐d‐glucan 6(3)‐α‐d‐glucosyltransferase; EC 2.4.1.140) is produced with the genetically modified Escherichia coli strain EBASSC by Advanced Enzyme Technologies Ltd. The production strain of the food enzyme contains a known antimicrobial resi...
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| Language: | English |
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Wiley
2025-04-01
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| Series: | EFSA Journal |
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| Online Access: | https://doi.org/10.2903/j.efsa.2025.9331 |
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| author | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Giulio Di Piazza Cristina Fernàndez‐Fraguas Natalia Kovalkovičová Silvia Peluso Yi Liu |
| author_facet | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Giulio Di Piazza Cristina Fernàndez‐Fraguas Natalia Kovalkovičová Silvia Peluso Yi Liu |
| author_sort | EFSA Panel on Food Enzymes (FEZ) |
| collection | DOAJ |
| description | Abstract The food enzyme alternansucrase (sucrose:1,6(1,3)‐α‐d‐glucan 6(3)‐α‐d‐glucosyltransferase; EC 2.4.1.140) is produced with the genetically modified Escherichia coli strain EBASSC by Advanced Enzyme Technologies Ltd. The production strain of the food enzyme contains a known antimicrobial resistance gene. However, based on the absence of viable cells and DNA from the production organism in the food enzyme, this is not considered to be a risk. The food enzyme is intended to be used in the processing of sugars for the production of maltose alternan gluco‐oligosaccharides. Two sets of dietary exposure estimates were made, using the default or more specific input factors. The refined dietary exposure was estimated to be up to 0.586 mg total organic solids (TOS)/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90‐day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 500 mg TOS/kg bw per day, the mid‐dose tested, which when compared with the refined exposure estimate, results in a margin of exposure of at least 853. A search for the homology of the amino acid sequence of the alternansucrase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use. |
| format | Article |
| id | doaj-art-9e9ec0c77e9d49f49a9a021cb8a7c23a |
| institution | Kabale University |
| issn | 1831-4732 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | EFSA Journal |
| spelling | doaj-art-9e9ec0c77e9d49f49a9a021cb8a7c23a2025-08-20T03:33:17ZengWileyEFSA Journal1831-47322025-04-01234n/an/a10.2903/j.efsa.2025.9331Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSCEFSA Panel on Food Enzymes (FEZ)Holger ZornJosé Manuel Barat BavieraClaudia BolognesiFrancesco CataniaGabriele GadermaierRalf GreinerBaltasar MayoAlicja MortensenYrjö Henrik RoosMarize L. M. SolanoMonika SramkovaHenk Van LoverenLaurence VernisMagdalena AndryszkiewiczDaniele CavannaGiulio Di PiazzaCristina Fernàndez‐FraguasNatalia KovalkovičováSilvia PelusoYi LiuAbstract The food enzyme alternansucrase (sucrose:1,6(1,3)‐α‐d‐glucan 6(3)‐α‐d‐glucosyltransferase; EC 2.4.1.140) is produced with the genetically modified Escherichia coli strain EBASSC by Advanced Enzyme Technologies Ltd. The production strain of the food enzyme contains a known antimicrobial resistance gene. However, based on the absence of viable cells and DNA from the production organism in the food enzyme, this is not considered to be a risk. The food enzyme is intended to be used in the processing of sugars for the production of maltose alternan gluco‐oligosaccharides. Two sets of dietary exposure estimates were made, using the default or more specific input factors. The refined dietary exposure was estimated to be up to 0.586 mg total organic solids (TOS)/kg body weight (bw) per day in European populations. Genotoxicity tests did not indicate a safety concern. The systemic toxicity was assessed by means of a repeated dose 90‐day oral toxicity study in rats. The Panel identified a no observed adverse effect level of 500 mg TOS/kg bw per day, the mid‐dose tested, which when compared with the refined exposure estimate, results in a margin of exposure of at least 853. A search for the homology of the amino acid sequence of the alternansucrase to known allergens was made and no match was found. The Panel considered that a risk of allergic reactions upon dietary exposure to the food enzyme cannot be excluded, but the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns, under the intended conditions of use.https://doi.org/10.2903/j.efsa.2025.9331alternansucraseEC 2.4.1.140EFSA‐Q‐2023‐00555Escherichia colifood enzymegenetically modified microorganism |
| spellingShingle | EFSA Panel on Food Enzymes (FEZ) Holger Zorn José Manuel Barat Baviera Claudia Bolognesi Francesco Catania Gabriele Gadermaier Ralf Greiner Baltasar Mayo Alicja Mortensen Yrjö Henrik Roos Marize L. M. Solano Monika Sramkova Henk Van Loveren Laurence Vernis Magdalena Andryszkiewicz Daniele Cavanna Giulio Di Piazza Cristina Fernàndez‐Fraguas Natalia Kovalkovičová Silvia Peluso Yi Liu Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC EFSA Journal alternansucrase EC 2.4.1.140 EFSA‐Q‐2023‐00555 Escherichia coli food enzyme genetically modified microorganism |
| title | Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC |
| title_full | Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC |
| title_fullStr | Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC |
| title_full_unstemmed | Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC |
| title_short | Safety evaluation of the food enzyme alternansucrase from the genetically modified Escherichia coli strain EBASSC |
| title_sort | safety evaluation of the food enzyme alternansucrase from the genetically modified escherichia coli strain ebassc |
| topic | alternansucrase EC 2.4.1.140 EFSA‐Q‐2023‐00555 Escherichia coli food enzyme genetically modified microorganism |
| url | https://doi.org/10.2903/j.efsa.2025.9331 |
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