miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM)
Breast cancer (BC), the most prevalent and fatal female neoplasm worldwide, including in Malaysia, is a heterogeneous disease classified into subtypes based on hormone receptor status, including luminal A, luminal B, HER2-enriched and triple-negative. The heterogeneity of BC poses challenges for acc...
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Biome Scientia
2025-05-01
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| Series: | Life Sciences, Medicine and Biomedicine |
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| Online Access: | https://biomescientia.com/index.php/lsmb/article/view/171 |
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| author | Norlizawati Abdul Hamed Xiao Ning Zhu Bann Siang Yeo Wen Xuan Lee Geok Chin Tan Yoke Kqueen Cheah |
| author_facet | Norlizawati Abdul Hamed Xiao Ning Zhu Bann Siang Yeo Wen Xuan Lee Geok Chin Tan Yoke Kqueen Cheah |
| author_sort | Norlizawati Abdul Hamed |
| collection | DOAJ |
| description | Breast cancer (BC), the most prevalent and fatal female neoplasm worldwide, including in Malaysia, is a heterogeneous disease classified into subtypes based on hormone receptor status, including luminal A, luminal B, HER2-enriched and triple-negative. The heterogeneity of BC poses challenges for accurate diagnosis and treatment, necessitating novel biomarkers to aid cancer screening, diagnosis and therapy. MicroRNAs (miRNAs), which regulate gene expression, are often dysregulated in cancer. Specifically, miR-125b has been linked to mitochondrial dysfunction and chemoresistance, supporting its potential as a biomarker for BC, particularly as a predictive biomarker. This study aims to investigate the associations between miR-125b expression patterns in different BC subtypes and demographic profiles at Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia (HCTM). miR-125b expression was analysed in 18 formalin-fixed paraffin-embedded (FFPE) BC tissue samples and two control tissues by quantitative polymerase chain reaction (qPCR). Among 241 BC cases reported at HCTM in 2023, patients were predominantly Malay, aged 50 and above, with luminal A being the most common subtype, followed by triple-negative, luminal B and HER2-enriched. miR-125b expression was consistently downregulated in BC tissues compared to controls, although this difference was not statistically significant (p = 0.263). Trends of downregulation were observed across all subtypes and demographic groups, with no significant differences by age (p = 1.000), ethnicity (p = 0.546) or BC subtypes (p = 0.701). The consistent downregulation of miR-125b aligns with previous studies and highlights its biomarker potential for BC diagnosis and prognosis. Further research with a larger cohort is needed to validate these findings and explore the potential of miR-125b as a diagnostic, prognostic or predictive biomarker in BC. |
| format | Article |
| id | doaj-art-9e9ada3ef3af4f50b26f103492509c13 |
| institution | OA Journals |
| issn | 2600-7207 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Biome Scientia |
| record_format | Article |
| series | Life Sciences, Medicine and Biomedicine |
| spelling | doaj-art-9e9ada3ef3af4f50b26f103492509c132025-08-20T02:15:20ZengBiome ScientiaLife Sciences, Medicine and Biomedicine2600-72072025-05-019110.28916/lsmb.9.1.2025.171149miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM)Norlizawati Abdul Hamed0Xiao Ning Zhu1Bann Siang Yeo2Wen Xuan Lee3Geok Chin Tan4Yoke Kqueen Cheah5Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Department of Hepatobiliary, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, 646000 Luzhou, Sichuan, China.Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia.UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.Breast cancer (BC), the most prevalent and fatal female neoplasm worldwide, including in Malaysia, is a heterogeneous disease classified into subtypes based on hormone receptor status, including luminal A, luminal B, HER2-enriched and triple-negative. The heterogeneity of BC poses challenges for accurate diagnosis and treatment, necessitating novel biomarkers to aid cancer screening, diagnosis and therapy. MicroRNAs (miRNAs), which regulate gene expression, are often dysregulated in cancer. Specifically, miR-125b has been linked to mitochondrial dysfunction and chemoresistance, supporting its potential as a biomarker for BC, particularly as a predictive biomarker. This study aims to investigate the associations between miR-125b expression patterns in different BC subtypes and demographic profiles at Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia (HCTM). miR-125b expression was analysed in 18 formalin-fixed paraffin-embedded (FFPE) BC tissue samples and two control tissues by quantitative polymerase chain reaction (qPCR). Among 241 BC cases reported at HCTM in 2023, patients were predominantly Malay, aged 50 and above, with luminal A being the most common subtype, followed by triple-negative, luminal B and HER2-enriched. miR-125b expression was consistently downregulated in BC tissues compared to controls, although this difference was not statistically significant (p = 0.263). Trends of downregulation were observed across all subtypes and demographic groups, with no significant differences by age (p = 1.000), ethnicity (p = 0.546) or BC subtypes (p = 0.701). The consistent downregulation of miR-125b aligns with previous studies and highlights its biomarker potential for BC diagnosis and prognosis. Further research with a larger cohort is needed to validate these findings and explore the potential of miR-125b as a diagnostic, prognostic or predictive biomarker in BC.https://biomescientia.com/index.php/lsmb/article/view/171breast cancermicrornamir-125bdemographicmalaysia |
| spellingShingle | Norlizawati Abdul Hamed Xiao Ning Zhu Bann Siang Yeo Wen Xuan Lee Geok Chin Tan Yoke Kqueen Cheah miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) Life Sciences, Medicine and Biomedicine breast cancer microrna mir-125b demographic malaysia |
| title | miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) |
| title_full | miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) |
| title_fullStr | miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) |
| title_full_unstemmed | miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) |
| title_short | miR-125b expression in breast cancer: Insights into subtypes and demographic factors at Hospital Canselor Tuanku Muhriz (HCTM) |
| title_sort | mir 125b expression in breast cancer insights into subtypes and demographic factors at hospital canselor tuanku muhriz hctm |
| topic | breast cancer microrna mir-125b demographic malaysia |
| url | https://biomescientia.com/index.php/lsmb/article/view/171 |
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