Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines
Mitochondria play a crucial role in oxidative stress control and reactive oxygen species (ROS) generation, impacting many cellular processes. Dysregulated mitochondria are linked to diseases such as colorectal cancer (CRC), known for its aggressiveness. Since ROS plays a role in tumor growth and met...
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MDPI AG
2025-05-01
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| Online Access: | https://www.mdpi.com/2075-1729/15/5/743 |
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| author | Laura Garcia-Sureda Camille Jacques Daniel G. Pons Jorge Sastre-Serra Jordi Oliver Ilaria Floris |
| author_facet | Laura Garcia-Sureda Camille Jacques Daniel G. Pons Jorge Sastre-Serra Jordi Oliver Ilaria Floris |
| author_sort | Laura Garcia-Sureda |
| collection | DOAJ |
| description | Mitochondria play a crucial role in oxidative stress control and reactive oxygen species (ROS) generation, impacting many cellular processes. Dysregulated mitochondria are linked to diseases such as colorectal cancer (CRC), known for its aggressiveness. Since ROS plays a role in tumor growth and metastasis, there is considerable interest in developing therapies that target these reactives. This study investigates the effects of some active substances from the micro-immunotherapy (MI) medicine 2LMIREG<sup>®</sup> on mitochondrial metabolism parameters in two CRC-derived cell lines. HT-29 and the metastasis-derived SW620 cell lines, which heavily rely on ROS for proliferation, were used to evaluate the effects of the tested active substances. Cellular viability and various mitochondrial metabolism parameters were measured: ROS production, mitochondrial mass index, and mitochondrial DNA levels. In both cell lines, the tested MI formulation reduced cellular viability as well as ROS production compared to the vehicle used as a control. The treatment also appeared to increase the mitochondrial mass index without affecting mitochondrial DNA levels in the two CRC models. Altogether, these preliminary results report for the first time the mitochondria-related effects of some actives from 2LMIREG<sup>®</sup> in two CRC cell models and open perspectives for further in-depth metabolism-based studies. |
| format | Article |
| id | doaj-art-9e9096f163034dcb8e817c652f5e2c22 |
| institution | OA Journals |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-9e9096f163034dcb8e817c652f5e2c222025-08-20T01:56:16ZengMDPI AGLife2075-17292025-05-0115574310.3390/life15050743Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell LinesLaura Garcia-Sureda0Camille Jacques1Daniel G. Pons2Jorge Sastre-Serra3Jordi Oliver4Ilaria Floris5Preclinical Research Department, Labo’life España, 07330 Consell, SpainPreclinical Research Department, Labo’life France, Pescalis-Les Magnys, 79320 Moncoutant-sur-Sevre, FranceGrupo Multidisciplinar de Oncología Traslacional, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainGrupo Multidisciplinar de Oncología Traslacional, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainGrupo Multidisciplinar de Oncología Traslacional, Institut Universitari d’Investigació en Ciències de la Salut (IUNICS), Universitat de les Illes Balears, 07122 Palma de Mallorca, SpainPreclinical Research Department, Labo’life France, Pescalis-Les Magnys, 79320 Moncoutant-sur-Sevre, FranceMitochondria play a crucial role in oxidative stress control and reactive oxygen species (ROS) generation, impacting many cellular processes. Dysregulated mitochondria are linked to diseases such as colorectal cancer (CRC), known for its aggressiveness. Since ROS plays a role in tumor growth and metastasis, there is considerable interest in developing therapies that target these reactives. This study investigates the effects of some active substances from the micro-immunotherapy (MI) medicine 2LMIREG<sup>®</sup> on mitochondrial metabolism parameters in two CRC-derived cell lines. HT-29 and the metastasis-derived SW620 cell lines, which heavily rely on ROS for proliferation, were used to evaluate the effects of the tested active substances. Cellular viability and various mitochondrial metabolism parameters were measured: ROS production, mitochondrial mass index, and mitochondrial DNA levels. In both cell lines, the tested MI formulation reduced cellular viability as well as ROS production compared to the vehicle used as a control. The treatment also appeared to increase the mitochondrial mass index without affecting mitochondrial DNA levels in the two CRC models. Altogether, these preliminary results report for the first time the mitochondria-related effects of some actives from 2LMIREG<sup>®</sup> in two CRC cell models and open perspectives for further in-depth metabolism-based studies.https://www.mdpi.com/2075-1729/15/5/743micro-immunotherapymitochondriaHT-29 cell lineSW620 cell linein vitroreactive oxygen species |
| spellingShingle | Laura Garcia-Sureda Camille Jacques Daniel G. Pons Jorge Sastre-Serra Jordi Oliver Ilaria Floris Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines Life micro-immunotherapy mitochondria HT-29 cell line SW620 cell line in vitro reactive oxygen species |
| title | Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines |
| title_full | Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines |
| title_fullStr | Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines |
| title_full_unstemmed | Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines |
| title_short | Active Substances from the Micro-Immunotherapy Medicine 2LMIREG Display Antioxidative Properties In Vitro in Two Colorectal Cancer Cell Lines |
| title_sort | active substances from the micro immunotherapy medicine 2lmireg display antioxidative properties in vitro in two colorectal cancer cell lines |
| topic | micro-immunotherapy mitochondria HT-29 cell line SW620 cell line in vitro reactive oxygen species |
| url | https://www.mdpi.com/2075-1729/15/5/743 |
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