RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells
Abstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proli...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-81815-2 |
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| _version_ | 1850110025095184384 |
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| author | Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao |
| author_facet | Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | Abstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC. |
| format | Article |
| id | doaj-art-9e901890976f4abebe1bc0cef4f9bfae |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-9e901890976f4abebe1bc0cef4f9bfae2025-08-20T02:37:55ZengNature PortfolioScientific Reports2045-23222021-01-0111111010.1038/s41598-021-81815-2RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cellsYuan Wang0Huazhang Wu1Nannan Dong2Xu Su3Mingxiu Duan4Yaqin Wei5Jun Wei6Gaofeng Liu7Qingjie Peng8Yunli Zhao9School of Public Health, Bengbu Medical CollegeSchool of Life Science, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical CollegeSchool of Public Health, Bengbu Medical CollegeSchool of Life Science, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical CollegeSchool of Public Health, Bengbu Medical CollegeSchool of Clinical Medicine, Bengbu Medical CollegeDepartment of Gastroenterology, The First Affiliated Hospital of Bengbu Medical CollegeSchool of Life Science, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical CollegeSchool of Life Science, Anhui Province Key Laboratory of Translational Cancer Research, Bengbu Medical CollegeSchool of Public Health, Bengbu Medical CollegeAbstract Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC.https://doi.org/10.1038/s41598-021-81815-2 |
| spellingShingle | Yuan Wang Huazhang Wu Nannan Dong Xu Su Mingxiu Duan Yaqin Wei Jun Wei Gaofeng Liu Qingjie Peng Yunli Zhao RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells Scientific Reports |
| title | RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells |
| title_full | RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells |
| title_fullStr | RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells |
| title_full_unstemmed | RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells |
| title_short | RETRACTED ARTICLE: Sulforaphane induces S-phase arrest and apoptosis via p53-dependent manner in gastric cancer cells |
| title_sort | retracted article sulforaphane induces s phase arrest and apoptosis via p53 dependent manner in gastric cancer cells |
| url | https://doi.org/10.1038/s41598-021-81815-2 |
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