Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?

The urine culture is the “gold standard” for the diagnosis of urinary tract infections (UTI) but constitutes a significant workload in the routine clinical laboratory. Due to the high percentage of negative results, there is a need for an efficient screening method, with a high negative predictive v...

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Main Authors: Patricia Mejuto, Mariam Luengo, Julio Díaz-Gigante
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:International Journal of Microbiology
Online Access:http://dx.doi.org/10.1155/2017/8532736
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author Patricia Mejuto
Mariam Luengo
Julio Díaz-Gigante
author_facet Patricia Mejuto
Mariam Luengo
Julio Díaz-Gigante
author_sort Patricia Mejuto
collection DOAJ
description The urine culture is the “gold standard” for the diagnosis of urinary tract infections (UTI) but constitutes a significant workload in the routine clinical laboratory. Due to the high percentage of negative results, there is a need for an efficient screening method, with a high negative predictive value (NPV) that could reduce the number of unnecessary culture tests. With the purpose of improving the efficiency of laboratory work, several methods for screening out the culture-negative samples have been developed, but none of them has shown adequate sensitivity (SE) and high NPV. Many authors show data about the efficacy of flow cytometry in the routine clinical laboratory. The aim of this article is to review and discuss the current literature on the feasibility of urine flow cytometry (UFC) and its utility as an alternative analytical technique in urinalysis.
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series International Journal of Microbiology
spelling doaj-art-9e8def1ee2294f7baf2207d43d5748ca2025-08-20T03:38:42ZengWileyInternational Journal of Microbiology1687-918X1687-91982017-01-01201710.1155/2017/85327368532736Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?Patricia Mejuto0Mariam Luengo1Julio Díaz-Gigante2Department of Microbiology, Hospital del Oriente de Asturias Francisco Grande Covián, Arriondas, Asturias, SpainDepartment of Biochemistry, Hospital del Oriente de Asturias Francisco Grande Covián, Arriondas, Asturias, SpainDepartment of Microbiology, Hospital del Oriente de Asturias Francisco Grande Covián, Arriondas, Asturias, SpainThe urine culture is the “gold standard” for the diagnosis of urinary tract infections (UTI) but constitutes a significant workload in the routine clinical laboratory. Due to the high percentage of negative results, there is a need for an efficient screening method, with a high negative predictive value (NPV) that could reduce the number of unnecessary culture tests. With the purpose of improving the efficiency of laboratory work, several methods for screening out the culture-negative samples have been developed, but none of them has shown adequate sensitivity (SE) and high NPV. Many authors show data about the efficacy of flow cytometry in the routine clinical laboratory. The aim of this article is to review and discuss the current literature on the feasibility of urine flow cytometry (UFC) and its utility as an alternative analytical technique in urinalysis.http://dx.doi.org/10.1155/2017/8532736
spellingShingle Patricia Mejuto
Mariam Luengo
Julio Díaz-Gigante
Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
International Journal of Microbiology
title Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
title_full Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
title_fullStr Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
title_full_unstemmed Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
title_short Automated Flow Cytometry: An Alternative to Urine Culture in a Routine Clinical Microbiology Laboratory?
title_sort automated flow cytometry an alternative to urine culture in a routine clinical microbiology laboratory
url http://dx.doi.org/10.1155/2017/8532736
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AT juliodiazgigante automatedflowcytometryanalternativetourinecultureinaroutineclinicalmicrobiologylaboratory